Centaurin alpha 1 (ADAP1) (NM_001284311) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CENTA1; GCS1L; p42IP4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284311, the custom clone sequence may differ by one or more nucleotides ATGGTATTGGCTTCACTCGAGCTCCTTCGAGAGCAGTGGATCCGGGCCAAGTACGAGCGACAGGAGTTCA TCTACCCGGAGAAGCAGGAGCCCTACTCGGCAGGGTACCGTGAGGGTTTTCTCTGGAAGCGTGGCCGGGA CAACGGGCAGTTTTTGAGCCGGAAGTTTGTGCTGACAGAACGAGAGGGTGCTCTGAAGTATTTCAACAGA AATGATGCCAAGGAGCCCAAGGCCGTGATGAAGATCGAGCACCTGAACGCCACCTTCCAGCCGGCCAAGA TCGGCCACCCCCACGGCCTGCAGGTCACCTACCTGAAGGACAACAGCACCCGTAACATCTTCATCTACCA TGAGGACGGGAAGGAGATTGTGGACTGGTTCAATGCACTCCGAGCTGCTCGCTTCCACTACCTGCAGGTG GCATTCCCAGGGGCCAGCGACGCAGATCTGGTGCCAAAGCTCTCCAGGAACTACCTGAAGGAAGGCTACA TGGAGAAGACGGGGCCCAAGCAAACGGAAGGCTTCCGGAAGCGCTGGTTCACCATGGATGACCGCAGGCT CATGTACTTCAAAGACCCCCTGGACGCCTTCGCCCGAGGGGAAGTCTTCATTGGCAGCAAGGAGAGTGGC TACACGGTGCTGCATGGGTTCCCGCCGTCCACCCAGGGCCACCACTGGCCACATGGCATCACCATCGTCA CGCCCGACCGCAAGTTTCTGTTTGCCTGCGAGACGGAGTCCGACCAGAGGGAGTGGGTGGCGGCCTTCCA GAAGGCGGTGGACAGGCCCATGCTGCCCCAGGAGTACGCAGTGGAGGCGCACTTCAAGCATAAACCTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001284311
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284311.1, NP_001271240.1
RefSeq Size	1963 bp
RefSeq ORF	840 bp
Locus ID	11033
UniProt ID	O75689
Protein Families	Druggable Genome
Gene Summary	GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) lacks 2 exons at the 5' end and contains an alternate 5' terminal exon, which results in translation initiation from an alternate start codon compared to variant 1. The resulting shorter isoform (4) has a distinct N-terminus compared to isoform 1.

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