EI24 (NM_001290135) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EPG4; PIG8; TP53I8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335349 representing NM_001290135. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGACAGTGTCAAAACCTTTCTCCAGGACCTTGCCAGACTAGATGCTCGAATCCAGCAAAAGAGA GAGGAGCAGCGTCGAAGAAGGGCAAGTAGTGTCTTGGCACAGAGAAGAGCCCAGAGTATAGAGCGGAAG CAAGAGAGTGAGCCACGTATTGTTAGTAGAATTTTCCAGTGTTGTGCTTGGAATGGTGGAGTGTTCTGG TTCAGTCTCCTCTTGTTTTATCGAGTATTTATTCCTGTGCTTCAGTCGGTAACAGCCCGAATTATCGGT GACCCATCACTACATGGAGATGTTTGGTCGTGGCTGGAATTCTTCCTCACGTCAATTTTCAGTGCTCTT TGGGTGCTCCCCTTGTTTGTGCTTAGCAAAGTGGTGAATGCCATTTGGTTTCAGGATATAGCTGACCTG GCATTTGAGGTATCAGGGAGGAAGCCTCACCCATTCCCTAGTGTCAGCAAAATAATTGCTGACATGCTC TTCAACCTTTTGCTGCAGGCTCTTTTCCTCATTCAGGGAATGTTTGTGAGTCTCTTTCCCATCCATCTT GTCGGTCAGCTGGTTAGTCTCCTGCATATGTCCCTTCTCTACTCACTGTACTGCTTTGAATATCGTTGG TTCAATAAAGGAATTGAAATGCACCAGCGGTTGTCTAACATAGAAAGGAATTGGCCTTACTACTTTGGG TTTGGTTTGCCCTTGGCTTTTCTCACAGCAATGCAGTCCTCATATATTATCAGTGGCTGCCTTTTCTCT ATCCTCTTTCCTTTATTCATTATCAGCGCCAATGAAGCAAAGACCCCTGGCAAAGCATATCTCTTCCAG TTGCGCCTCTTCTCCTTGGTGGTCTTCTTAAGCAACAGACTCTTCCACAAGACAGTCTACCTGCAGTCG GCCCTGAGCAGCTCTACTTCTGCAGAGAAGTTCCCTTCACCGCATCCGTCGCCTGCCAAACTGAAGGCT ACTGCAGGTCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001290135
Insert Size	981 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290135.1
RefSeq Size	2244 bp
RefSeq ORF	981 bp
Locus ID	9538
UniProt ID	O14681
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	p53 signaling pathway
MW	37.5 kDa
Gene Summary	This gene encodes a putative tumor suppressor and has higher expression in p53-expressing cells than in control cells and is an immediate-early induction target of p53-mediated apoptosis. The encoded protein may suppress cell growth by inducing apoptotic cell death through the caspase 9 and mitochondrial pathways. This gene is located on human chromosome 11q24, a region frequently altered in cancers. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, 7, and 8. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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