PTDSS1 (NM_001290225) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LMHD; PSS1; PSSA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335354 representing NM_001290225. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGTATGCTGTGAACTGCCATGTGATCACCTGGGAGAGGATTATCAGCCACTTTGATATTTTTGCA TTTGGACATTTCTGGGGCTGGGCCATGAAGGCCTTGCTGATCCGTAGTTACGGTCTCTGCTGGACAATC AGTATTACCTGGGAGCTGACTGAGCTCTTCTTCATGCATCTCCTCCCCAATTTTGCCGAGTGCTGGTGG GATCAAGTCATTCTGGACATCCTGTTGTGCAATGGCGGTGGCATTTGGCTGGGCATGGTCGTTTGCCGG TTTTTAGAGATGAGGACTTACCACTGGGCAAGCTTCAAGGACATTCATACCACCACCGGGAAGATCAAG AGAGCTGTTCTGCAGTTCACTCCTGCTAGCTGGACCTATGTTCGATGGTTTGACCCCAAATCTTCTTTT CAGAGAGTAGCTGGAGTGTACCTTTTCATGATCATCTGGCAGCTGACTGAGTTGAATACCTTCTTCTTG AAGCATATCTTTGTGTTCCAAGCCAGTCATCCATTAAGTTGGGGTAGAATTCTCTTTATTGGTGGCATC ACAGCTCCCACAGTGAGACAGTACTACGCTTACCTCACCGACACACAGTGCAAGCGCGTAGGAACACAA TGCTGGGTGTTTGGGGTCATTGGTTTCCTGGAGGCCATTGTTTGCATAAAATTTGGACAAGATCTCTTC TCTAAGACCCAAATACTCTATGTTGTGCTTTGGCTTCTTTGCGTGGCTTTCACCACTTTCCTCTGTCTG TACGGCATGATTTGGTATGCAGAACACTATGGTCACCGAGAAAAGACCTACTCGGAGTGTGAAGATGGC ACCTACAGTCCAGAGATCTCCTGGCATCACAGGAAAGGGACAAAAGGTTCTGAAGACAGCCCACCCAAG CATGCAGGCAACAACGAAAGCCATTCTTCCAGGAGAAGGAATCGGCATTCCAAGTCAAAAGTCACCAAT GGCGTTGGAAAGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001290225
Insert Size	984 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290225.1
RefSeq Size	2406 bp
RefSeq ORF	984 bp
Locus ID	9791
UniProt ID	P48651
Protein Families	Transmembrane
Protein Pathways	Glycerophospholipid metabolism, Metabolic pathways
MW	38.3 kDa
Gene Summary	The protein encoded by this gene catalyzes the formation of phosphatidylserine from either phosphatidylcholine or phosphatidylethanolamine. Phosphatidylserine localizes to the mitochondria-associated membrane of the endoplasmic reticulum, where it serves a structural role as well as a signaling role. Defects in this gene are a cause of Lenz-Majewski hyperostotic dwarfism. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (2) lacks two consecutive alternate exons compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

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