MAEA (NM_001297430) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EMLP; EMP; GID9; HLC-10; P44EMLP; PIG5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335367 representing NM_001297430. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGTGCAGGAGTCGGCGGCTCAGTTGTCCATGACCCTGAAGGTCCAGGAGTACCCGACCCTCAAG GTGCCCTACGAGACGCTGAACAAACGCTTTCGCGCCGCTCAGAAGAACATTGACCGGGAGACCAGCCAC GTCACCATGGTGGTGGCCGAGCTGGAGAAGACGTTGAGCGGCTGCCCCGCCGTGGACTCCGTGGTCAGC CTGCTGGACGGCGTGGTGGAGAAGCTCAGCGTCCTCAAGAGGAAGGACCTAGTGAATATTGAGATGTTC CTGACGGCCAAAGAGGTGGAGGAGTCCCTGGAGAGGCGTGAGACGGCCACCTGCCTGGCCTGGTGCCAT GACAACAAGTCCCGGCTCCGGAAGATGAAGAGCTGCCTGGAGTTCAGCCTCAGAATCCAGGAGTTCATT GAACTCATCCGGCAGAATAAGAGACTGGACGCTGTGAGACATGCAAGAAAGCACTTCAGCCAAGCAGAA GGGAGCCAGCTGGACGAGGTGCGCCAGGCCATGGGCATGCTGGCCTTCCCGCCCGACACGCACATCTCC CCGTACAAGGACCTTCTGGACCCTGCACGGTGGCGGATGCTGATCCAGCAGTTCCGGTACGACAACTAC CGACTACACCAGCTGGGAAACAATTCTGTGTTCACCCTCACCCTGCAGGCTGGCCTCTCAGCCATCAAG ACACCACAGTGCTACAAGGAGGACGGCAGCTCCAAGAGCCCTGACTGCCCTGTGTGCAGCCGCTCCCTG AACAAGCTGGCGCAGCCCCTGCCCATGGCCCACTGTGCCAACTCCCGCCTGGTCTGCAAGATTTCTGGC GACGTGATGAACGAGAACAATCCGCCCATGATGCTGCCCAACGGCTACGTCTACGGCTACAATTCTCTG CTTTCTATCCGTCAAGATGATAAAGTCGTGTGCCCGAGAACCAAAGAAGTCTTCCACTTCTCACAAGCC GAGAAGGTGTACATCATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297430
Insert Size	987 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297430.1
RefSeq Size	2046 bp
RefSeq ORF	987 bp
Locus ID	10296
Protein Families	Druggable Genome
MW	37.4 kDa
Gene Summary	This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (3) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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