LACTB (NM_001288585) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	G24; MRPL56
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335399 representing NM_001288585. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTACCGGCTCATGTCAGCAGTGACTGCCCGGGCTGCCGCCCCCGGGGGCTTGGCCTCAAGCTGCGGA CGACGCGGGGTCCATCAGCGCGCCGGGCTGCCGCCTCTCGGCCACGGCTGGGTCGGGGGCCTCGGGCTG GGGCTGGGGCTGGCGCTCGGGGTGAAGCTGGCAGGTGGGCTGAGGGGCGCGGCCCCGGCGCAGTCCCCC GCGGCCCCCGACCCTGAGGCGTCGCCTCTGGCCGAGCCGCCACAGGAGCAGTCCCTCGCCCCGTGGTCT CCGCAGACCCCGGCGCCGCCCTGCTCCAGGTGCTTCGCCAGAGCCATCGAGAGCAGCCGCGACCTGCTG CACAGGATCAAGGATGAGGTGGGCGCACCGGGCATAGTGGTTGGAGTTTCTGTAGATGGAAAAGAAGTC TGGTCAGAAGGTTTAGGTTATGCTGATGTTGAGAACCGTGTACCATGTAAACCAGAGACAGTTATGCGA ATTGCTAGCATCAGCAAAAGTCTCACCATGGTTGCTCTTGCCAAATTGTGGGAAGCAGGGAAACTGGAT CTTGATATTCCAGTACAACATTATGTTCCCGAATTCCCAGAAAAAGAATATGAAGGTGAAAAGGTTTCT GTCACAACAAGATTACTGATTTCCCATTTAAGTGGAATTCGTCATTATGAAAAGGACATAAAAAAGGTG AAAGAAGAGAAAGCTTATAAAGCCTTGAAGATGATGAAAGAGAATGTTGCATTTGAGCAAGAAAAAGAA GGCAAAAGTAATGAAAAGAATGATTTTACTAAATTTAAAACAGAGCAGGAGAATGAAGCCAAATGCCGG AATTCAAAACCTGGCAAGAAAAAGAATGATTTTGAACAAGGCGAATTATATTTGAGAGAAAAGTTTGAA AATTCAATTGAATCCCTAAGATTATTTAAAAATGATCCTTTGTTCTTCAAACCTGTCAGTTTTTGTATT CAACTTTTGGCTATACCCTACTGGCAGCCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288585
Insert Size	999 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288585.1
RefSeq Size	3007 bp
RefSeq ORF	999 bp
Locus ID	114294
UniProt ID	P83111
Protein Families	Protease
MW	36.5 kDa
Gene Summary	This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (3) contains multiple differences in the 3' coding region and 3' UTR, compared to variant 1, one of which results in a frameshift. The encoded isoform (c) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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