COX18 (NM_001300729) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	COX18HS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335433 representing NM_001300729. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGCTTTATGCCCGCTGTTGCTGGCGCGTGCGCGTATCAGCCTTGGCTGGCCGGTGTATGTCCGCTG GATTGTGAGGCCCGGGATCTGGGCTCCGCCGTGGTGCAGAAATGCTGTGCCGGCTCGGCGGTCGGTGGC TGCGGCCGCTCCCTGCCCTGCAGCTTTGGGCTAGGGACCTGCCGCTTGCGCCGGTTCCTACGAGCGGCG CCAAGCGCCCCACTCTCCCAGTGTGGGCAGTGGCACCAGTCTCTGCAGTACATGCGAACGGCTGGTACG AGGCCCTGGCCGCGTCTTCGCCGGTGCGGGTTGCGGAGGAAGTACTGCTCGGCGTGCACGCCGCCACGG GCCTGCCCTGGTGGGGCAGCATTCTGCTCTCCACCGTGGCCTTACGGGGTGCTGTCACGCTGCCTTTGG CAGCCTACCAGCACTACATCCTGGCCAAGGCTCACTTATCTAAAGAATATGAGGAGGCTAATTTCAGAG CTATATGTGCGAGATAACTGCCACCCTTTCAAAGCCACTGTGTTGGTTTGGATTCAGCTTCCAATGTGG ATCTTCATGTCTTTTGCTCTCCGGAATTTAAGCACGGGGGCAGCACATTCAGAAGGTTTTTCTGTTCAG GAACAGTTAGCTACTGGTGGAATTCTGTGGTTTCCTGACCTCACTGCACCCGACTCCACTTGGATTCTG CCTATCTCTGTTGGCGTCATCAATTTGTTAATAGTGGAGATTTGTGCTCTACAAAAAATTGGAATGTCT CGTTTTCAGACGTATATTACGTACTTTGTCCGTGCAATGTCGGTGTTGATGATACCAATTGCTGCAACG GTACCCTCATCAATTGTTCTCTACTGGTTATGCTCCAGCTTCGTGGGCCTTTCACAGAATTTGCTGCTG CGTTCTCCTGGATTTCGCCAACTTTGCCGAATACCATCGACCAAGTCAGATTCAGAAACTCCTTATAAA GACATATTTGCTGCCTTTAATACCAAGTTCATTTCAAGAAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300729
Insert Size	1011 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300729.1
RefSeq Size	4429 bp
RefSeq ORF	1011 bp
Locus ID	285521
UniProt ID	Q8N8Q8
Protein Families	Transmembrane
MW	37.3 kDa
Gene Summary	This gene encodes a cytochrome c oxidase assembly protein. The encoded protein is essential for integral membrane protein insertion into the mitochondrial inner membrane. It is also required for cytochrome c oxidase assembly and activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (4) lacks an exon in the central coding region, which results in the use of an alternate start codon and a frameshift, compared to variant 1. The encoded isoform (4) has a longer and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.