NDRG2 (NM_001282212) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SYLD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335474 representing NM_001282212. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGAGCTGCAGGAGGTGCAGATCACAGAGGAGAAGCCACTGTTGCCAGGACAGACGCCTGAGGCG GCCAAGACTCACTCTGTGGAGACACCATACGGCTCTGTCACTTTCACTGTCTATGGCACCCCCAAACCC AAACGCCCAGCGATCCTTACCTACCACGATGTGGGACTCAACTATAAATCTTGCTTCCAGCCACTGTTT CAGTTCGAGGACATGCAGGAAATCATTCAGAACTTTGTGCGGGTTCATGTGGATGCCCCTGGAATGGAA GAGGGAGCCCCTGTGTTCCCTTTGGGATATCAGTACCCATCTCTGGACCAGCTTGCAGACATGATCCCT TGCGTCCTGCAGTACCTAAATTTCTCTACAATAATTGGAGTTGGTGTTGGAGCTGGAGCCTACATCCTG GCGAGATATGCTCTTAACCACCCGGACACTGTTGAAGGTCTTGTCCTCATCAACATTGATCCCAATGCC AAGGGTTGGATGGATTGGGCAGCCCACAAGCTAACAGGCCTCACCTCTTCCATTCCGGAGATGATCCTT GGACATCTTTTCAGCCAGGAAGAGCTCTCTGGAAATTCTGAGTTGATACAAAAGTACAGAAATATCATT ACACATGCACCCAACCTGGATAACATTGAATTGTACTGGAACAGCTACAACAACCGCCGAGACCTGAAC TTTGAGCGTGGAGGTGATATCACCCTCAGGTGTCCTGTGATGCTGGTGGTAGGAGACCAAGCACCTCAT GAAGATGCAGTGATGGCTGACTCCGGAGGTCAGCCCCAGCTGACTCAGCCAGGCAAGCTGACCGAGGCC TTCAAGTACTTCCTGCAAGGCATGGGCTACATGGCCTCATCCTGCATGACTCGCCTGTCCCGGTCTCGT ACAGCCTCTCTGACCAGTGCAGCATCCGTTGATGGCAACCGGTCCCGCTCTCGCACCCTGTCCCAGAGC AGCGAGTCTGGAACTCTTTCTTCGGGGCCCCCGGGGCACACCATGGAGGTCTCCTGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282212
Insert Size	1026 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282212.1
RefSeq Size	2204 bp
RefSeq ORF	1026 bp
Locus ID	57447
UniProt ID	Q9UN36
MW	37.5 kDa
Gene Summary	This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (10) differs in the 5' UTR and lacks two alternate in-frame exons compared to variant 1. The resulting isoform (d) lacks two alternate internal segments compared to isoform a.

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