PHF19 (NM_001286842) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MTF2L1; PCL3; TDRD19B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335681 representing NM_001286842. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGCAATGTTACCGGTGCAGGCAGTGGTTCCACGAGGCCTGCACCCAGTGCCTCAATGAGCCCATG ATGTTTGGAGACCGGTTTTACCTGTTCTTCTGCTCCGTGTGTAACCAGGGCCCAGAGTACATCGAGAGG CTGCCCCTGCGATGGGTGGATGTGGTTCACCTGGCCCTCTATAATCTGGGGGTACAGAGCAAGAAGAAG TACTTTGACTTTGAGGAGATTCTGGCCTTTGTCAACCACCACTGGGAGCTCCTGCAGCTTGGCAAGCTC ACCAGCACCCCAGTGACAGATCGAGGACCACATCTCCTCAACGCTCTGAACAGTTATAAAAGCCGGTTC CTCTGCGGCAAGGAGATCAAGAAGAAGAAGTGCATCTTCCGCCTGCGCATCCGCGTCCCACCCAACCCG CCAGGGAAGCTGCTGCCTGACAAAGGACTGCTGCCAAATGAGAACAGCGCCTCCTCTGAGCTGCGTAAG AGAGGAAAGAGCAAGCCTGGTTTGTTGCCTCACGAATTCCAGCAGCAGAAAAGGCGAGTTTATAGAAGA AAAAGATCAAAGTTTTTGCTGGAAGATGCTATTCCCAGTAGTGACTTCACCTCAGCCTGGAGCACCAAC CACCACCTGGCTAGCATATTTGACTTCACGCTGGATGAAATTCAAAGTTTAAAAAGTGCCAGCTCAGGC CAGACCTTCTTCTCAGATGTCGACTCCACCGACGCTGCCAGCACCTCTGGCTCTGCCTCCACCAGCCTC TCCTATGACTCCAGATGGACAGTGGGCAGCCGAAAGAGGAAGCTGGCAGCCAAGGCATACATGCCCCTG CGGGCAAAGCGGTGGGCAGCTGAGCTGGATGGACGCTGCCCCTCGGACAGCAGTGCAGAGGGGGCTTCA GTCCCCGAGCGGCCAGACGAAGGCATTGACAGCCACACATTTGAGAGCATCAGTGAAGATGACTCATCC CTGTCCCACCTCAAGTCATCTATCACCAACTACTTTGGTGCAGCTGGGCGGTTGGCCTGTGGGGAGAAG TACCAGGTGTTGGCTCGGAGGGTCACACCTGAGGGCAAGGTTCAGTACCTGGTGGAGTGGGAAGGGACC ACCCCTTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286842
Insert Size	1116 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286842.1
RefSeq Size	3547 bp
RefSeq ORF	1116 bp
Locus ID	26147
Protein Families	Druggable Genome
MW	42.2 kDa
Gene Summary	Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks several 5' exons but includes an alternate 5' terminal exon, and it thus differs in its 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 3. The encoded isoform (d) is shorter at the N-terminus, compared to isoform c.

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