DIPK1A (NM_001252271) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FAM69A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335894 representing NM_001252271. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAATATCTTTTCTTTTCCTGGTTAGTGGTTTTTGTTGGAAGCTGGATTATATATGTGCAGTATTCT ACCTATACAGAATTATGCAGAGGAAAGGACTGTAAGAAAATAATATGTGACAAGTACAAGACTGGAGTT ATTGATGGGCCTGCATGTAACAGCCTTTGTGTTACAGAAACTCTTTACTTTGGAAAATGTTTATCCACC AAGCCCAACAATCAGATGTATTTAGGGATTTGGGATAATCTACCAGGTGTTGTGAAATGTCAAATGGAA CAAGCGCTTCATCTTGATTTTGGAACTGAATTGGAACCAAGAAAAGAAATAGTGCTATTTGATAAGCCA ACTAGAGGAACTACTGTACAAAAATTTAAAGAAATGGTCTATAGTCTCTTTAAGGCAAAATTGGGTGAC CAAGGAAACCTCTCTGAACTGGTTAATCTCATCTTGACGGTGGCTGATGGAGACAAAGATGGCCAGGTT TCCTTGGGAGAAGCAAAGTCGGCATGGGCACTTCTTCAACTGAATGAATTTCTTCTCATGGTGATACTT CAAGATAAAGAACATACCCCCAAATTAATGGGATTCTGTGGTGACCTCTATGTGATGGAAAGTGTTGAA TATACCTCTCTTTATGGAATAAGCCTTCCTTGGGTCATTGAACTTTTTATTCCATCTGGGTTCAGAAGA AGCATGGATCAGCTGTTCACACCATCATGGCCAAGAAAGGCCAAAATAGCCATAGGACTTCTAGAATTT GTGGAAGATGTTTTCCATGGCCCCTACGGAAATTTCCTCATGTGCGATACTAGTGCCAAAAACCTAGGA TATAATGATAAGTATGATTTGAAAATGGTGGATATGAGAAAAATTGTGCCAGAGACAAACCTGAAAGAA CTTATTAAGGATCGTCACTGTGAGTCTGATTTGGACTGTGTCTATGGCACAGATTGTAGAACTAGCTGT GATCAGAGTACAATGAAGTGTACTTCAGAAGTGATACAACCAAACTTGGCAAAAGCTTGTCAGTTACTC AAAGACTACCTACTGCGTGGTGCTCCAAGTGAAATTCGTGAAGAATTAGAAAAGCAGCTTTATTCTTGT ATTGCTCTCAAAGTCACAGCAAATCAAATGGAAATGGAACATTCTTTGATACTAAATAACCTAAAAACA TTATTGTGGAAGAAAATTTCCTACACTAATGACTCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001252271
Insert Size	1212 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252271.1
RefSeq Size	2646 bp
RefSeq ORF	1212 bp
Locus ID	388650
Protein Families	Transmembrane
MW	46 kDa
Gene Summary	This gene encodes a member of the FAM69 family of cysteine-rich type II transmembrane proteins. These proteins localize to the endoplasmic reticulum but their specific functions are unknown. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and uses a downstream, in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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