AGXT2L2 (PHYKPL) (NM_001278346) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AGXT2L2; PHLU
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335926 representing NM_001278346. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTACGATGAACAGGGGGCAGAATACATCGATTGCATCAGCAATGTGGCGCACGTTGGGCACTGCCAC CCTCTCGTGGTCCAAGCAGCACATGAGCAGAACCAGGTGCTCAACACCAACAGCCGGTACCTGCATGAC AACATCGTGGACTATGCGCAGAGGCTGTCAGAGACCCTGCCGGAGCAGCTCTGTGTGTTCTATTTCCTG AATTCTGGGTCAGAAGCCAATGACCTGGCCCTGAGGCTGGCTCGCCACTACACGGGACACCAGGACGTG GTGGTATTAGATCATGCGTATCACGGCCACCTGAGCTCCCTGATTGACATCAGTCCCTACAAGTTCCGC AACCTGGATGGCCAGAAGGAGTGGGTCCACGTGGCACCTCTCCCAGACACCTACCGGGGCCCCTACCGG GAGGACCACCCCAACCCAGCTATGGCCTATGCCAACGAGGTGAAACGTGTGGTCAGCAGTGCACAGGAG AAGGGCAGGAAGATTGCAGCCTTCTTCGCTGAGTCTCTGCCCAGTGTGGGAGGGCAGATCATTCCCCCT GCTGGCTACTTCTCCCAAGTGGCAGAGCACATCCGCAAGGCCGGAGGGGTCTTTGTTGCAGATGAGATC CAGGTTGGCTTTGGCCGGGTAGGCAAGCACTTCTGGGCCTTCCAGCTCCAGGGAAAAGACTTCGTCCCT GACATCGTCACCATGGGCAAGTCCATTGGCAACGGCCACCCTGTTGCCTGCGTGGCCGCAACCCAGCCT GTGGCGAGGGCATTTGAAGCCACCGGCGTTGAGTACTTCAACACGTTTGGGGGCAGCCCAGTGTCCTGC GCTGTGGGGCTGGCCGTCCTGAATGTCTTGGAGAAGGAGCAGCTCCAGGATCATGCCACCAGTGTAGGC AGCTTCCTGATGCAGCTCCTCGGGCAGCAAAAAATCAAACATCCCATCGTCGGGGATGTCAGGGGTGTT GGGCTCTTCATTGGTGTGGATCTGATCAAAGATGAGGCCACAAGGACACCAGCAACTGAAGAGGCTGCC TACTTGGTATCAAGGCTGAAGGAGAACTACGTTTTGCTGAGCACTGATGGCCCTGGGAGGAACATCCTG AAGTTTAAGCCCCCAATGTGCTTCAGCCTGGACAATGCACGGCAGGTGGTGGCAAAGCTGGATGCCATT CTGACTGACATGGAAGAGAAGGTGAGAAGTTGTGAAACGCTGAGGCTCCAGCCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278346
Insert Size	1230 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278346.1
RefSeq Size	2921 bp
RefSeq ORF	1230 bp
Locus ID	85007
UniProt ID	Q8IUZ5
Protein Families	Druggable Genome
MW	45.1 kDa
Gene Summary	This is a nuclear gene encoding a mitochondrial enzyme that catalyzes the conversion of 5-phosphonooxy-L-lysine to ammonia, inorganic phosphate, and 2-aminoadipate semialdehyde. Mutations in this gene may cause phosphohydroxylysinuria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] Transcript Variant: This variant (3) uses an alternate splice site at the 5' exon and initiates translation at a downstream in-frame start codon, compared to variant 2. The encoded isoform (3) has a shorter N-terminus, compared to isoform 2.

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