Tuftelin 1 (TUFT1) (NM_001301317) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335933 representing NM_001301317. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCAGTCCAGGAATGACCTCTCTACAACTAGAGAAAGAAGAATGCATGCAATGTGTTCCCTGTAGT CCAAGAACCAGGGTAGCCCTGCTGGAGCTCTACAGAGCCATGTGCCAGGGCAGCGTGGACATTCTCAGG CTGACTCTCCAGGGTGAACTGACAGGAGATGAACTTGAACACATAGCCCAGAAGGCGGGCAGGAAGACC TATGCCATGGTGTCCAGCCACTCAGCTGGTCATTCTCTGGCTTCAGAACTGGTGGAGTCCCATGATGGA CATGAGGAGATCATTAAGGTGTACTTGAAGGGGAGGTCTGGAGACAAGATGATTCACGAGAAGAATATT AACCAGCTGAAGAGTGAGGTCCAGTACATCCAGGAGGCCAGGAACTGCCTACAGAAGCTCCGGGAGGAT ATAAGTAGCAAGCTTGACAGGAACCTAGGAGATTCTCTCCATCGACAGGAGATACAGGTGGTGCTAGAA AAGCCAAATGGCTTTAGTCAGAGTCCCACAGCCCTGTACAGCAGCCCACCTGAGGTGGACACCTGTATA AATGAGGATGTTGAGAGCTTGAGGAAGACGGTGCAGGACTTGCTGGCCAAGCTTCAGGAGGCCAAGCGG CAACACCAGTCAGACTGTGTGGCTTTTGAGGTCACACTCAGCCGGTACCAGAGGGAAGCAGAACAAAGT AATGTGGCCCTTCAGAGAGAGGAGGACAGAGTGGAGCAGAAAGAGGCAGAAGTCGGAGAGCTGCAGAGG CGCTTGCTAGGGATGGAGACGGAGCATCAGGCCTTACTGGCGAAAGTGAGGGAAGGGGAGGTGGCCCTA GAGGAACTTCGGAGCAACAATGCTGACTGCCAAGCAGAACGAGAAAAGGCTGCTACCCTGGAAAAGGAA GTGGCCGGGTTGCGGGAGAAGATCCACCACTTGGATGACATGCTCAAGAGCCAGCAGCGGAAAGTCCGG CAAATGATAGAGCAGCTCCAGAATTCAAAAGCTGTGATCCAGTCAAAGGACGCCACCATCCAGGAGCTC AAGGAGAAAATCGCCTATCTGGAGGCAGAGAATTTAGAGATGCATGACCGGATGGAACACCTGATAGAA AAACAAATCAGTCATGGCAACTTCAGCACCCAGGCCCGGGCCAAGACAGAGAACCCGGGCAGTATTAGG ATATCCAAGCCGCCTAGCCCGAAGCCCATGCCTGTCATCCGAGTGGTGGAAACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301317
Insert Size	1230 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301317.1
RefSeq Size	3257 bp
RefSeq ORF	1230 bp
Locus ID	7286
UniProt ID	Q9NNX1
Protein Families	Secreted Protein
MW	46.4 kDa
Gene Summary	Tuftelin is an acidic protein that is thought to play a role in dental enamel mineralization and is implicated in caries susceptibility. It is also thought to be involved with adaptation to hypoxia, mesenchymal stem cell function, and neurotrophin nerve growth factor mediated neuronal differentiation. [provided by RefSeq, Aug 2014] Transcript Variant: This variant (3) includes an additional exon compared to variant 1. The transcript may be subject to nonsense mediated decay or may be translated from ribosomal leaky scanning. The inferred protein (isoform 3) has a distinct N-terminus compared to isoform 1.

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