SNRP70 (SNRNP70) (NM_001301069) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U1AP; U1RNP; U170K
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336064 representing NM_001301069. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCCAGTTCCTGCCGCCCAACCTTCTGGCCCTCTTTGCCCCCCGTGACCCTATTCCATACCTGCCA CCCCTGGAGAAACTGCCACATGAAAAACACCACAATCAACCTTATTGTGGCATTGCGCCGTACATTCGA GAGTTTGAGGACCCTCGAGATGCCCCTCCTCCAACTCGTGCTGAAACCCGAGAGGAGCGCATGGAGAGG AAAAGACGGGAAAAGATTGAGCGGCGACAGCAAGAAGTGGAGACAGAGCTTAAAATGTGGGACCCTCAC AATGATCCCAATGCTCAGGGGGATGCCTTCAAGACTCTCTTCGTGGCGAGAGTGAATTATGACACAACA GAATCCAAGCTCCGGAGAGAGTTTGAGGTGTACGGACCTATCAAAAGAATACACATGGTCTACAGTAAG CGGTCAGGAAAGCCCCGTGGCTATGCCTTCATCGAGTACGAACACGAGCGAGACATGCACTCCGCTTAC AAACACGCAGATGGCAAGAAGATTGATGGCAGGAGGGTCCTTGTGGACGTGGAGAGGGGCCGAACCGTG AAGGGCTGGAGGCCCCGGCGGCTAGGAGGAGGCCTCGGTGGTACCAGAAGAGGAGGGGCTGATGTGAAC ATCCGGCATTCAGGCCGCGATGACACCTCCCGCTACGATGAGAGGGACCGGGACCGGGACCGTGAGCGG GAGCGCAGAGAGCGGAGCCGGGAGCGAGACAAGGAGCGAGAACGGCGACGCTCCCGCTCCCGGGACCGG CGGAGGCGCTCACGGAGTCGCGACAAGGAGGAGCGGAGGCGCTCCAGGGAGCGGAGCAAGGACAAGGAC CGGGACCGGAAGCGGCGAAGCAGCCGGAGTCGGGAGCGGGCCCGGCGGGAGCGGGAGCGCAAGGAGGAG CTGCGTGGCGGCGGTGGCGACATGGCGGAGCCCTCCGAGGCGGGTGACGCGCCCCCTGATGATGGGCCT CCAGGGGAGCTCGGGCCTGACGGCCCTGACGGTCCAGAGGAAAAGGGCCGGGATCGTGACCGGGAGCGA CGGCGGAGCCACCGGAGCGAGCGCGAGCGGCGCCGGGACCGGGATCGTGACCGTGACCGTGACCGCGAG CACAAACGGGGGGAGCGGGGCAGTGAGCGGGGCAGGGATGAGGCCCGAGGTGGGGGCGGTGGCCAGGAC AACGGGCTGGAGGGTCTGGGCAACGACAGCCGAGACATGTACATGGAGTCTGAGGGCGGCGACGGCTAC CTGGCTCCGGAGAATGGGTATTTGATGGAGGCTGCGCCGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301069
Insert Size	1287 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301069.1
RefSeq Size	1981 bp
RefSeq ORF	1287 bp
Locus ID	6625
UniProt ID	P08621
Protein Families	Druggable Genome
Protein Pathways	Spliceosome
MW	50.6 kDa
Gene Summary	Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome (PubMed:19325628, PubMed:25555158). SNRNP70 binds to the loop I region of U1-snRNA (PubMed:2467746, PubMed:19325628, PubMed:25555158).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1.

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