LIPI (NM_001302999) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CT17; LPDL; mPA-PLA1 beta; PLA1C; PRED5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336080 representing NM_001302999. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAGTATACATTTTTCTTTGTTTGATGTGCTGGGTGAGATCTGATAATAAAAGACCATGCCTTGAA TTCTCTCAGCTAAGTGTAAAGGATTCCTTCAGAGATTTATTTATTCCGAGAATAGAGACCATTCTGATG ATGTATACAAGGAACAACCTAAACTGTGCTGAGCCACTGTTTGAACAAAATAACTCACTTAATGTTAAT TTCAACACACAAAAGAAAACAGTCTGGCTTATTCACGGATACAGACCAGTAGGCTCCATCCCATTATGG CTTCAGAACTTCGTAAGGATTTTGCTGAATGAAGAAGATATGAATGTAATTGTAGTAGACTGGAGCCGG GGTGCTACAACTTTTATTTATAATAGAGCAGTTAAAAACACCAGAAAAGTTGCTGTGAGTTTGAGTGTG CACATTAAAAATCTTTTGAAGCATGGTGCATCTCTTGACAATTTTCATTTCATAGGTGTGAGCTTAGGG GCTCATATCAGTGGATTTGTTGGAAAGATATTTCATGGTCAACTTGGAAGAATAACAGGTCTTGACCCT GCTGGGCCAAGGTTCTCCAGAAAACCACCATATAGCAGATTAGATTACACGGATGCAAAGTTTGTGGAT GTCATCCATTCTGACTCCAATGGAATTCAATTCATTAAATGCAACCACCAGAGAGCAGTTCACTTGTTC ATGGCATCTTTAGAAACAAACTGCAATTTTATTTCATTTCCTTGTCGTTCATACAAAGATTACAAGACT AGCTTATGTGTGGACTGTGACTGTTTTAAGGAAAAATCATGTCCTCGGCTGGGTTATCAAGCCAAGCTA TTTAAAGGTGTTTTAAAAGAAAGGATGGAAGGAAGACCTCTTAGGACCACTGTGTTTTTGGATACAAGT GGTACATATCCATTCTGTACCTATTATTTTGTTCTCAGTATAATTGTTCCAGATAAAACTATGATGGAT GGCTCGTTTTCATTTAAATTATTAAATCAGCTTGGAATGATTGAAGAGCCAAGGCTTTATGAAAAGAAC AAACCATTTTATAAACTTCAAGAAGTCAAGATTCTTGCTCAATTTTATAATGACTTTGTAAATATTTCA AGCATTGGTTTGACATATTTCCAGAGCTCAAATCTGCAGTGTTCCACATGCACATACAAGATCCAGAGT CTCATGTTAAAATCACTTACATACCCAGAAAGACCACCACTTTGCAGGTATAATATTGTACTTAAAGAC AGAGAGGAAGTGTTTCTTAATCCAAACACATGTACACCAAAGAACACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001302999
Insert Size	1293 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302999.1
RefSeq Size	1519 bp
RefSeq ORF	1293 bp
Locus ID	149998
UniProt ID	Q6XZB0
Protein Families	Secreted Protein
MW	49.8 kDa
Gene Summary	The protein encoded by this gene is a phospholipase that hydrolyzes phosphatidic acid to produce lysophosphatidic acid. Defects in this gene are a cause of susceptibility to familial hypertrigliceridemia. This gene is also expressed at high levels in Ewing family tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (deltaE5) lacks an alternate in-frame exon, compared to variant fl. The encoded isoform (2) is shorter than isoform 1.

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