Gamma tubulin complex component 3 (TUBGCP3) (NM_001286279) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	104p; ALP6; GCP3; Grip104; SPBC98; Spc98; Spc98p
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336135 representing NM_001286279. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGACCCCGGACCAGAAGTCGCCGAACGTTCTGCTGCAGAACCTGTGCTGCAGGATCCTGGGCAGG AGCGAAGCTGATGTAGCCCAGCAGTTCCAGTATGCTGTGCGGGTGATTGGCAGCAACTTCGCCCCAACT GTTGAAAGAGATGAATTTTTAGTAGCTGAAAAAATCAAGAAAGAGCTTATTCGACAACGAAGAGAAGCA GATGCTGCATTATTTTCAGAACTCCACAGAAAACTTCATTCACAGGGAGTTTTGAAAAATAAATGGTCA ATACTCTACCTCTTGCTGAGCCTCAGTGAGGACCCACGCAGGCAGCCAAGCAAGGTTTCTAGCTATGCT ACGTTATTTGCTCAGGCCTTACCAAGAGATGCCCACTCAACCCCTTACTACTATGCCAGGCCTCAGACC CTTCCCCTGAGCTACCAAGATCGGAGTGCCCAGTCAGCCCAGAGCTCCGGCAGCGTGGGCAGCAGTGGC ATCAGCAGCATTGGCCTGTGTGCCCTCAGTGGCCCCGCGCCTGCGCCACAATCTCTCCTCCCAGGACAG TCTAATCAAGCTCCAGGAGTAGGAGATTGCCTTCGACAGCAGTTGGGGTCACGACTCGCATGGACTTTA ACTGCAAATCAGCCTTCTTCACAAGCCACTACCTCAAAAGGTGTCCCCAGTGCTGTGTCTCGCAACATG ACAAGGTCCAGGAGAGAAGGGGATACGGGTGGTACTATGGAAATTACAGAAGCAGCTCTGGTAAGGGAC ATTTTGTACGTCTTTCAGGGCATAGATGGCAAAAACATCAAAATGAACAACACTGAAAATTGTTACAAA GTAGAAGGAAAGGCAAATCTAAGTAGGTCTTTGAGAGACACAGCAGTCAGGCTTTCTGAGTTGGGATGG TTGCATAATAAAATCAGAAGATACACGGACCAGAGGAGCCTGGACCGCTCATTCGGACTCGTCGGGCAG AGCTTTTGTGCTGCCTTGCACCAGGAACTCAGAGAATACTATCGATTGCTCTCTGTTTTACATTCTCAG CTACAACTAGAGGATGACCAGGGTGTGAATTTGGGACTTGAGAGTAGTTTAACACTTCGGCGCCTCCTG GTTTGGACCTATGATCCCAAAATACGACTGAAGACCCTTGCGGCCCTAGTGGACCACTGCCAAGGTCCC ACGCGCGTCTTTCCCACGCACGTCTTTCCCACGCGCGACTTTCCCACGCGCGACTTTCCCACGCGCGAC TTTCCCATGCACGTCTTTCCCACGCGCGTCTTTCCCACGCGCGTCTGGCACTCGCTTTGTTTCAGAACT CGTTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286279
Insert Size	1320 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286279.1
RefSeq Size	2529 bp
RefSeq ORF	1320 bp
Locus ID	10426
UniProt ID	Q96CW5
Protein Families	Druggable Genome
MW	49.3 kDa
Gene Summary	Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks multiple 3' exons and its 3' terminal exon extends past a splice site that is used in variant 1, resulting in a distinct 3' coding region and 3' UTR. The encoded isoform (4) has a distinct C-terminus and is shorter than isoform 1.

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