TCFL5 (NM_001301726) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bHLHe82; CHA; E2BP-1; Figlb; SOSF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336260 representing NM_001301726. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGGCCCCGGACCGCGGGAGCCGCCGCCGGAGGCAGGCGCGGCAGGCGGCGAGGCGGCCGTCGAG GGCGCGGGCGGCGGGGACGCGGCGCTGGGCGAGCCGGGGCTGAGCTTCACGACCACCGACCTGAGCCTG GTGGAGATGACGGAGGTGGAGTACACGCAGCTGCAGCACATCCTCTGCTCGCACATGGAGGCGGCGGCT GACGGCGAGCTCGAGACGCGCCTCAACTCGGCGCTGCTGGCGGCGGCGGGCCCGGGCGCAGGCGCGGGC GGCTTCGCGGCGGGCGGTCAGGGGGGCGCGGCGCCCGTGTACCCCGTGCTGTGCCCGTCCGCGCTGGCG GCCGACGCGCCCTGCCTGGGCCACATCGACTTCCAGGAGCTGCGCATGATGCTGCTAAGCGAGGCGGGC GCGGCGGAGAAGACGTCGGGCGGCGGGGACGGAGCGAGGGCCCGGGCCGACGGCGCCGCCAAGGAGGGC GCGGGCGCGGCTGCGGCTGCGGCTGGACCCGACGGCGCGCCCGAGGCCCGGGCCAAGCCGGCCGTGCGC GTCCGCCTGGAGGACCGCTTCAACAGCATCCCCGCCGAGCCGCCGCCCGCGCCGCGCGGCCCCGAGCCC CCCGAGCCGGGCGGGGCGCTCAACAATTTGGTAACTCTCATTCGACATCCATCTGAACTAATGAATGTT CCTCTTCAGCAACAAAACAAATGTACAGCATTAGTGAAAAATAAAACTGCGGCTACAACTACTGCTTTG CAATTTACATACCCACTGTTTACTACAAATGCTTGCTCTACTAGTGGAAATTCTAATCTTTCACAGACA CAGAGTTCTAGTAACTCATGTTCTGTACTTGAAGCTGCCAAGCACCAGGATATTGGATTGCCTAGAGCA TTTTCTTTCTGTTATCAGCAAGAAATTGAATCCACTAAACAGACGTTAGGTAGTAGAAACAAAGTTTTG CCTGAGCAAGTTTGGATTAAAGTGGGAGCAGCGCTATGCAAACAAGCACTGAAGAGGAATCGGAGTAGA ATGCGTCAGTTGGACACAAATGTAGAGCGAAGAGCCCTTGGAGAGATTCAGAATGTGGGCGAAGGTGCC ACCGCCACACAAGGCGCTTGGCAGTCCTCGGAGTCCTCACAGGCAAACCTGGGGGAGCAGGCCCAGAGT GGGCCCCAGGGAGGAAGGTCTCAACGTAGGGAGAGGCATAACCGAATGGAAAGAGATAGAAGGCGCAGA ATCCGCATTTGCTGTGATGAGTTGAATCTCTTAGTGCCGTTCTGCAATGCCGAGACTGACAAGGCCACA ACTCTGCAGTGGACCACAGCATTCCTGAAATACATCCAGGAAAGACATGGAGATTCTCTTAAAAAGACT GGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301726
Insert Size	1386 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301726.1
RefSeq Size	2880 bp
RefSeq ORF	1386 bp
Locus ID	10732
Protein Families	Druggable Genome, Transcription Factors
MW	48.4 kDa
Gene Summary	Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site and contains an alternate exon in the 3' coding region, which results in the use of a distinct stop codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

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