CD30 (TNFRSF8) (NM_001281430) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD30; D1S166E; Ki-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336389 representing NM_001281430. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCTGTTCCACGTCTGCCGTCAACTCCTGTGCCCGCTGCTTCTTCCATTCTGTCTGTCCGGCAGGG ATGATTGTCAAGTTCCCAGGCACGGCGCAGAAGAACACGGTCTGTGAGCCGGCTTCCCCAGGGGTCAGC CCTGCCTGTGCCAGCCCAGAGAACTGCAAGGAACCCTCCAGTGGCACCATCCCCCAGGCCAAGCCCACC CCGGTGTCCCCAGCAACCTCCAGTGCCAGCACCATGCCTGTAAGAGGGGGCACCCGCCTCGCCCAGGAA GCTGCTTCTAAACTGACGAGGGCTCCCGACTCTCCCTCCTCTGTGGGAAGGCCTAGTTCAGATCCAGGT CTGTCCCCAACACAGCCATGCCCAGAGGGGTCTGGTGATTGCAGAAAGCAGTGTGAGCCCGACTACTAC CTGGACGAGGCCGGCCGCTGCACGGCCTGCGTGAGCTGTTCTCGAGATGACCTTGTGGAGAAGACGCCA TGTGCATGGAACTCCTCCCGCACCTGCGAATGTCGACCTGGCATGATCTGTGCCACATCAGCCACCAAC TCCTGTGCCCGCTGTGTCCCCTACCCAATCTGTGCAGCAGAGACGGTCACCAAGCCCCAGGATATGGCT GAGAAGGACACCACCTTTGAGGCGCCACCCCTGGGGACCCAGCCGGACTGCAACCCCACCCCAGAGAAT GGCGAGGCGCCTGCCAGCACCAGCCCCACTCAGAGCTTGCTGGTGGACTCCCAGGCCAGTAAGACGCTG CCCATCCCAACCAGCGCTCCCGTCGCTCTCTCCTCCACGGGGAAGCCCGTTCTGGATGCAGGGCCAGTG CTCTTCTGGGTGATCCTGGTGTTGGTTGTGGTGGTCGGCTCCAGCGCCTTCCTCCTGTGCCACCGGAGG GCCTGCAGGAAGCGAATTCGGCAGAAGCTCCACCTGTGCTACCCGGTCCAGACCTCCCAGCCCAAGCTA GAGCTTGTGGATTCCAGACCCAGGAGGAGCTCAACGCTGAGGAGTGGTGCGTCGGTGACAGAACCCGTC GCGGAAGAGCGAGGGTTAATGAGCCAGCCACTGATGGAGACCTGCCACAGCGTGGGGGCAGCCTACCTG GAGAGCCTGCCGCTGCAGGATGCCAGCCCGGCCGGGGGCCCCTCGTCCCCCAGGGACCTTCCTGAGCCC CGGGTGTCCACGGAGCACACCAATAACAAGATTGAGAAAATCTACATCATGAAGGCTGACACCGTGATC GTGGGGACCGTGAAGGCTGAGCTGCCGGAGGGCCGGGGCCTGGCGGGGCCAGCAGAGCCCGAGTTGGAG GAGGAGCTGGAGGCGGACCATACCCCCCACTACCCCGAGCAGGAGACAGAACCGCCTCTGGGCAGCTGC AGCGATGTCATGCTCTCAGTGGAAGAGGAAGGGAAAGAAGACCCCTTGCCCACAGCTGCCTCTGGAAAG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281430
Insert Size	1452 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281430.2
RefSeq Size	3616 bp
RefSeq ORF	1452 bp
Locus ID	943
UniProt ID	P28908
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction
MW	50.9 kDa
Gene Summary	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 3) is shorter than isoform 1.

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