ASH2L (NM_001282272) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASH2; ASH2L1; ASH2L2; Bre2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336413 representing NM_001282272. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCAACTACAGTTTTCATTGCAACGTCTGCCATCACAGTGGGAATACCTATTTCCTCCGGAAGCAA GCAAACTTGAAGGAAATGTGCCTTAGTGCTTTGGCCAACCTGACATGGCAGTCCCGAACACAGGATGAA CATCCGAAGACAATGTTCTCCAAAGATAAGGATATTATACCATTTATTGATAAATACTGGGAGTGCATG ACAACCAGACAGAGACCTGGGAAAATGACTTGGCCAAATAACATTGTTAAAACAATGAGTAAAGAAAGA GATGTATTCTTGGTAAAGGAACACCCAGATCCAGGCAGTAAAGATCCAGAAGAAGATTACCCCAAATTT GGACTTTTGGATCAGGACCTTAGTAACATTGGTCCTGCTTATGACAACCAAAAACAGAGCAGTGCTGTG TCTACTAGTGGGAATTTAAATGGGGGAATTGCAGCAGGAAGCAGCGGAAAAGGACGAGGAGCCAAGCGC AAACAGCAGGATGGAGGGACCACAGGGACCACCAAGAAGGCCCGGAGTGACCCTTTGTTTTCTGCTCAG CGCCTTCCCCCTCATGGCTACCCATTGGAACACCCGTTTAACAAAGATGGCTATCGGTATATTCTAGCT GAGCCTGATCCGCACGCCCCTGACCCCGAGAAGCTGGAACTTGACTGCTGGGCAGGAAAACCTATTCCT GGAGACCTCTACAGAGCCTGCTTGTATGAACGGGTTTTGTTAGCCCTACATGATCGAGCTCCCCAGTTA AAGATCTCAGATGACCGGCTGACTGTGGTTGGAGAGAAGGGCTACTCTATGGTGAGGGCCTCTCATGGA GTACGGAAAGGTGCCTGGTATTTTGAAATCACTGTGGATGAGATGCCACCAGATACCGCTGCCAGACTG GGTTGGTCCCAGCCCCTAGGAAACCTTCAAGCTCCTTTAGGTTATGATAAATTTAGCTATTCTTGGCGG AGCAAAAAGGGAACCAAGTTCCACCAGTCCATTGGCAAACACTACTCTTCTGGCTATGGACAGGGAGAC GTCCTGGGATTTTATATTAATCTTCCTGAAGACACAGAGACAGCCAAGTCATTGCCAGACACATACAAA GATAAGGCTTTGATAAAATTCAAGAGTTATTTGTATTTTGAGGAAAAAGACTTTGTGGATAAAGCAGAG AAGAGCCTGAAGCAGACTCCCCATAGTGAGATAATATTTTATAAAAATGGTGTCAATCAAGGTGTGGCT TACAAAGATATTTTTGAGGGGGTTTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACGGTTTCCATT AACTTTGGACCATGCTTCAAGTATCCTCCGAAGGATCTCACTTACCGCCCTATGAGTGACATGGGCTGG GGCGCCGTGGTAGAGCACACCCTGGCTGACGTCTTGTATCACGTGGAGACAGAAGTGGATGGGAGGCGC AGTCCCCCATGGGAACCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282272
Insert Size	1470 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282272.1
RefSeq Size	2808 bp
RefSeq ORF	1470 bp
Locus ID	9070
Protein Families	Druggable Genome, Transcription Factors
MW	55.3 kDa
Gene Summary	Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (d) has a shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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