PPWD1 (NM_001278929) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336419 representing NM_001278929. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGGTGTTTGATGTAGTGAACTTTGACATGATCAACATGCTGAAACTTGGCTATTTTCCTGGACAG TGTGAGTGGATCTATTGCCCAGGGGATGCAATTTCTTCAGTTGCTGCTTCCGAAAAGAGTACAGGAAAA ATTTTCATTTATGATGGCCGAGGAGATAACCAGCCACTTCATATTTTTGACAAACTCCATACATCACCT CTTACTCAGATACGGCTAAACCCAGTTTACAAAGCAGTAGTGTCTTCTGACAAATCTGGGATGATTGAA TACTGGACTGGGCCTCCTCATGAATATAAATTCCCCAAAAATGTGAACTGGGAATATAAAACTGACACT GATTTATATGAATTTGCCAAGTGTAAGGCTTATCCAACCAGCGTATGTTTTTCACCAGATGGGAAGAAA ATAGCTACTATTGGTTCTGATAGAAAAGTTAGAATTTTCAGATTTGTAACTGGAAAACTCATGAGAGTC TTTGATGAATCACTAAGCATGTTTACTGAACTGCAACAGATGAGGCAACAGTTACCAGACATGGAATTT GGCCGACGAATGGCTGTAGAACGTGAGTTGGAGAAGGTTGATGCTGTAAGATTAATTAATATAGTTTTT GATGAAACTGGACACTTCGTGCTGTATGGAACAATGCTGGGCATTAAAGTTATAAATGTAGAGACAAAC CGGTGTGTGCGGATTTTAGGCAAACAAGAAAATATTAGAGTGATGCAATTGGCTTTGTTCCAGGGGATA GCCAAAAAGCATCGTGCTGCAACTACTATAGAAATGAAAGCTTCTGAAAATCCTGTTCTTCAGAATATT CAAGCTGACCCAACAATAGTCTGTACATCATTCAAAAAGAATAGATTTTATATGTTTACCAAACGAGAA CCAGAAGATACGAAAAGTGCAGATTCTGATCGAGATGTTTTTAATGAGAAACCTTCTAAAGAAGAAGTC ATGGCAGCTACTCAAGCTGAAGGACCTAAACGAGTTTCGGACAGTGCCATTATCCACACCAGCATGGGA GACATTCACACCAAACTTTTTCCTGTTGAGTGCCCTAAGACAGTGGAAAACTTCTGTGTTCACAGCAGA AATGGTTATTATAATGGGCATACATTTCACCGTATAATTAAGGGCTTTATGATTCAGACTGGAGATCCA ACAGGTACTGGTATGGGAGGAGAAAGCATATGGGGAGGAGAATTTGAAGATGAATTTCATTCAACATTA CGACATGACAGGCCATACACACTCAGCATGGCTAACGCGGGATCAAATACTAATGGATCCCAGTTTTTC ATAACGGTAGTACCAACGCCTTGGCTTGATAATAAGCATACAGTATTTGGACGAGTGACTAAAGGAATG GAAGTTGTACAGAGGATCTCCAACGTCAAAGTCAATCCCAAAACAGATAAGCCCTATGAGGATGTCAGC ATCATAAATATTACTGTCAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278929
Insert Size	1473 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278929.1
RefSeq Size	2076 bp
RefSeq ORF	1473 bp
Locus ID	23398
UniProt ID	Q96BP3
MW	55.7 kDa
Gene Summary	PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be involved in pre-mRNA splicing (PubMed:11991638).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks an exon in the 5' region and uses a downstream start codon, compared to variant 1. It encodes isoform 4 which has a shorter N-terminus compared to isoform 1.

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