WASF3 (NM_001291965) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Brush-1; SCAR3; WAVE3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336475 representing NM_001291965. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTTTAGTGAAGAGGAACATTGAGCCCCGGCACTTGTGCCGGGGAGCTCTGCCTGAAGGGATTACC AGCGAACTTGAATGTGTAACCAATAGTACTCTTGCCGCTATCATACGCCAGCTGAGCAGTCTGAGCAAA CATGCTGAAGACATATTTGGTGAGTTGTTTAATGAGGCTAACAACTTCTACATCAGAGCAAATTCTCTT CAAGACAGAATTGATCGCCTTGCTGTCAAAGTCACCCAGCTGGATTCAACAGTGGAAGAGGTCTCACTA CAGGATATCAACATGAAAAAAGCTTTCAAAAGTTCCACAGTCCAAGACCAGCAAGTGGTTTCAAAGAAC AGCATTCCTAATCCTGTTGCTGATATTTACAACCAGAGTGATAAGCCACCGCCTCTGAACATCCTGACA CCATACAGAGATGACAAGAAGGATGGGCTGAAGTTCTATACTGATCCTTCCTATTTCTTTGACCTCTGG AAAGAAAAAATGCTACAGGACACAGAAGACAAAAGGAAAGAGAAAAGGCGTCAAAAGAGAGAGAAACAC AAGCTGAACCCTAACAGAAACCAGCAAGTAAATGTGAGAAAAGTCAGAACAAGAAAAGAAGAGTGGGAG AGAAGGAAAATGGGCATTGAGTTTATGAGTGACGCAAAGAAACTGGAGCAGGCAGGGAGCGCCAAAGAG GACAGAGTGCCCAGCGGGTCACATGCATCGGACGTTACGGATTACTCTTACCCGGCTACTCCCAACCAT TCTCTGCACCCCCAGCCTGTGACCCCTTCCTATGCAGCTGGTGACGTGCCACCACACGGGCCTGCAAGC CAGGCTGCGGAGCATGAGTACCGGCCCCCATCTGCCTCGGCGAGGCACATGGCCCTCAACAGACCTCAG CAGCCGCCCCCCCCGCCTCCCCCTCAGGCCCCAGAGGGGTCCCAGGCCTCTGCACCGATGGCTCCAGCA GACTACGGGATGCTCCCAGCGCAGATAATTGAGTATTACAACCCATCCGGACCACCTCCTCCGCCACCT CCTCCTGTGATTCCCTCAGCACAAACTGCCTTCGTCAGCCCTCTCCAGATGCCCATGCAGCCCCCGTTC CCTGCATCAGCCAGCTCCACGCACGCAGCTCCTCCTCACCCACCCTCCACCGGGCTCCTGGTCACAGCC CCGCCACCCCCGGGCCCACCACCTCCCCCGCCAGGCCCTCCTGGTCCCGGGTCTTCTCTTTCGTCCTCC CCAATGCATGGCCCCCCAGTAGCTGAGGCGAAGCGGCAAGAGCCTGCACAGCCACCAATCAGTGATGCT CGAAGCGACCTCCTCGCTGCTATTCGAATGGGAATTCAACTGAAAAAGGTGCAGGAGCAGCGGGAGCAG GAGGCCAAGCGGGAGCCAGTGGGGAATGACGTGGCCACGATCCTGTCCCGGCGCATTGCCGTGGAGTAC AGCGACTCTGACGACGACTCAGAGTTCGACGAGAACGACTGGTCCGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291965
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291965.1
RefSeq Size	4841 bp
RefSeq ORF	1500 bp
Locus ID	10810
UniProt ID	Q9UPY6
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Fc gamma R-mediated phagocytosis
MW	55 kDa
Gene Summary	This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants [provided by RefSeq, May 2014] Transcript Variant: This variant (2) lacks one exon and includes one alternate exon in the central coding region, compared to variant 1. The encoded isoform (2) has a shorter and distinct internal segment, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.