PAF49 (CD3EAP) (NM_001297590) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASE-1; ASE1; CAST; CD3EAP; PAF49; RPA34
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336544 representing NM_001297590. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGAGCCCCAGGCCGGCGGTGAGGATGCTGCTCGGTTCTCTTGTCCCCCCAACTTTACCGCGAAG CCCCCAGCCTCAGAGTCCCCTCGTTTCTCCTTGGAGGCGCTGACGGGTCCAGATACGGAGCTGTGGCTT ATTCAGGCCCCTGCAGACTTTGCCCCAGAATGCTTCAATGGGCGGCATGTGCCTCTCTCTGGCTCCCAG ATCGTCAAGGGCAAATTGGCAGGCAAGCGGCACCGCTATCGAGTCCTCAGCAGCTGTCCCCAAGCTGGA GAAGCGACCCTGCTGGCCCCCTCAACGGAGGCAGGAGGTGGACTCACCTGTGCCTCAGCCCCCCAGGGC ACCCTAAGGATCCTTGAGGGTCCCCAGCAATCCCTGTCAGGGAGCCCTCTGCAGCCCATCCCAGCAAGT CCCCCACCACAGATCCCTCCTGGCCTGAGGCCTCGGTTCTGTGCCTTTGGGGGCAACCCACCAGTCACA GGGCCTAGGTCAGCCTTGGCCCCCAACCTGCTCACCTCAGGGAAGAAGAAAAAGGAGATGCAGGTGACA GAGGCCCCAGTCACTCAGGAGGCAGTGAATGGGCACGGGGCCCTGGAGGTGGACATGGCTTTGGGGTCG CCAGAAATGGATGTGCGGAAGAAGAAGAAGAAAAAAAATCAGCAGCTGAAAGAACCAGAGGCAGCAGGG CCTGTGGGGACAGAGCCCACAGTGGAGACACTGGAGCCTCTGGGAGTGCTGTTCCCGTCCACCACCAAG AAGAGGAAGAAGCCCAAAGGGAAAGAAACCTTCGAGCCAGAAGACAAGACAGTGAAGCAGGAACAGATT AACACTGAGCCTCTAGAAGACACAGTCCTGTCCCCGACCAAAAAGAGAAAGAGGCAAAAGGGGACGGAA GGGATGGAGCCAGAGGAGGGGGTGACAGTTGAGTCTCAGCCACAGGTGAAGGTGGAGCCACTGGAGGAA GCCATCCCTCTGCCCCCTACGAAGAAGAGGAAAAAAGAAAAGGGACAGATGGCAATGATGGAGCCAGGG ACGGAGGCGATGGAGCCAGTGGAGCCGGAGATGAAGCCTCTGGAGTCCCCAGGGGGGACCATGGCGCCT CAACAGCCAGAAGGAGCGAAGCCTCAGGCCCAGGCAGCTCTGGCAGCTCCCAAAAAGAAGACGAAGAAA GAAAAACAGCAAGATGCCACAGTGGAGCCAGAGACAGAGGTGGTGGGGCCTGAGCTGCCGGATGACCTT GAGCCTCAGGCAGCTCCCACATCCACCAAGAAGAAGAAGAAGAAGAAAGAGAGAGGTCACACAGTGACT GAGCCAATTCAGCCACTAGAGCCTGAACTGCCAGGGGAGGGACAGCCTGAAGCCAGGGCAACTCCGGGA TCCACCAAGAAGAGGAAGAAGCAGAGTCAGGAAAGCCGGATGCCAGAGACAGTGCCCCAAGAGGAGATG CCAGGGCCGCCACTGAATTCAGAGTCTGGGGAGGAGGCTCCCACAGGCCGGGACAAGAAGCGGAAGCAG CAGCAGCAGCAGCCTGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297590
Insert Size	1539 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297590.1
RefSeq Size	3292 bp
RefSeq ORF	1539 bp
Locus ID	10849
UniProt ID	O15446
Protein Families	Transcription Factors
MW	55.2 kDa
Gene Summary	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.