GALNT2 (NM_001291866) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDG2T; GalNAc-T2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336651 representing NM_001291866. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGACAGAGAGGACTGGAATGAAATTGACCCCATTAAAAAGAAAGACCTTCATCACAGCAATGGA GAAGAGAAAGCACAAAGCATGGAGACCCTCCCTCCAGGGAAAGTACGGTGGCCAGACTTTAACCAGGAA GCTTATGTTGGAGGGACGATGGTCCGCTCCGGGCAGGACCCTTACGCCCGCAACAAGTTCAACCAGGTG GAGAGTGATAAGCTTCGAATGGACAGAGCCATCCCTGACACCCGGCATGACCAGTGTCAGCGGAAGCAG TGGCGGGTGGATCTGCCGGCCACCAGCGTGGTGATCACGTTTCACAATGAAGCCAGGTCGGCCCTACTC AGGACCGTGGTCAGCGTGCTTAAGAAAAGCCCGCCCCATCTCATAAAAGAAATCATCTTGGTGGATGAC TACAGCAATGATCCTGAGGACGGGGCTCTCTTGGGGAAAATTGAGAAAGTGCGAGTTCTTAGAAATGAT CGACGAGAAGGCCTCATGCGCTCACGGGTTCGGGGGGCCGATGCTGCCCAAGCCAAGGTCCTGACCTTC CTGGACAGTCACTGCGAGTGTAATGAGCACTGGCTGGAGCCCCTCCTGGAAAGGGTGGCGGAGGACAGG ACTCGGGTTGTGTCACCCATCATCGATGTCATTAATATGGACAACTTTCAGTATGTGGGGGCATCTGCT GACTTGAAGGGCGGTTTTGATTGGAACTTGGTATTCAAGTGGGATTACATGACGCCTGAGCAGAGAAGG TCCCGGCAGGGGAACCCAGTCGCCCCTATAAAAACCCCCATGATTGCTGGTGGGCTGTTTGTGATGGAT AAGTTCTATTTTGAAGAACTGGGGAAGTACGACATGATGATGGATGTGTGGGGAGGAGAGAACCTAGAG ATCTCGTTCCGCGTGTGGCAGTGTGGTGGCAGCCTGGAGATCATCCCGTGCAGCCGTGTGGGACACGTG TTCCGGAAGCAGCACCCCTACACGTTCCCGGGTGGCAGTGGCACTGTCTTTGCCCGAAACACCCGCCGG GCAGCAGAGGTCTGGATGGATGAATACAAAAATTTCTATTATGCAGCAGTGCCTTCTGCTAGAAACGTT CCTTATGGAAATATTCAGAGCAGATTGGAGCTTAGGAAGAAACTCAGCTGCAAGCCTTTCAAATGGTAC CTTGAAAATGTCTATCCAGAGTTAAGGGTTCCAGACCATCAGGATATAGCTTTTGGGGCCTTGCAGCAG GGAACTAACTGCCTCGACACTTTGGGACACTTTGCTGATGGTGTGGTTGGAGTTTATGAATGTCACAAT GCTGGGGGAAACCAGGAATGGGCCTTGACGAAGGAGAAGTCGGTGAAGCACATGGATTTGTGCCTTACT GTGGTGGACCGGGCACCGGGCTCTCTTATAAAGCTGCAGGGCTGCCGAGAAAATGACAGCAGACAGAAA TGGGAACAGATCGAGGGCAACTCCAAGCTGAGGCACGTGGGCAGCAACCTGTGCCTGGACAGTCGCACG GCCAAGAGCGGGGGCCTAAGCGTGGAGGTGTGTGGCCCGGCCCTTTCGCAGCAGTGGAAGTTCACGCTC AACCTGCAGCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291866
Insert Size	1602 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291866.1
RefSeq Size	4562 bp
RefSeq ORF	1602 bp
Locus ID	2590
UniProt ID	Q10471
Protein Families	Secreted Protein, Transmembrane
Protein Pathways	Metabolic pathways, O-Glycan biosynthesis
MW	60.8 kDa
Gene Summary	This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 2.

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