YTHDF3 (NM_001277816) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DF3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336659 representing NM_001277816. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCAGATCCATACATGCCTAGTTACTATGCTCCATCCATTGGATTTCCATATTCTCTTGGGGAAGCA GCGTGGTCCACAGCTGGAGACCAGCCTATGCCATATCTGACAACCTATGGACAAATGAGTAATGGAGAA CATCACTATATACCAGATGGTGTATTTAGTCAACCTGGGGCATTAGGAAATACCCCTCCATTTCTTGGT CAACATGGATTTAACTTTTTTCCTGGTAATGCTGATTTCTCTACATGGGGGACAAGTGGATCTCAGGGA CAATCAACACAAAGTTCTGCTTATAGTAGCAGTTATGGCTATCCACCTAGTTCTCTTGGGAGAGCTATT ACTGATGGACAGGCTGGATTTGGCAATGATACTTTGAGTAAGGTGCCTGGCATTAGCAGTATTGAGCAA GGCATGACTGGACTGAAAATTGGTGGTGACCTGACAGCTGCAGTGACAAAAACTGTAGGTACAGCTTTG AGCAGCAGTGGTATGACTAGCATTGCAACCAATAGTGTGCCCCCAGTTAGCAGTGCAGCACCTAAACCA ACCTCCTGGGCTGCCATTGCCAGAAAGCCTGCCAAACCTCAACCGAAACTTAAACCCAAGGGCAATGTG GGAATTGGGGGTTCTGCTGTACCACCACCTCCTATAAAACACAACATGAATATTGGAACTTGGGATGAA AAAGGGTCAGTGGTAAAGGCTCCACCAACCCAACCAGTTCTGCCTCCTCAAACTATAATCCAGCAGCCT CAGCCATTAATTCAACCACCACCATTGGTGCAAAGCCAACTGCCTCAACAGCAGCCTCAACCACCACAA CCACAGCAGCAACAAGGACCTCAGCCACAGGCCCAGCCTCACCAAGTGCAGCCTCAACAGCAGCAGCTG CAGAATCGCTGGGTAGCTCCTCGTAACAGGGGAGCAGGCTTCAACCAGAACAATGGAGCGGGCAGTGAA AACTTTGGTTTAGGTGTTGTACCTGTCAGTGCTTCACCTTCTAGTGTAGAAGTGCATCCCGTGCTGGAA AAGCTAAAGGCCATAAACAACTATAATCCCAAAGACTTTGATTGGAATCTGAAGAATGGACGTGTGTTT ATAATTAAAAGCTACTCTGAGGATGACATACATCGTTCCATTAAATACTCTATCTGGTGTAGTACTGAG CATGGTAATAAGCGTTTGGATGCAGCTTACCGTTCCCTGAATGGGAAAGGCCCACTCTATTTACTCTTC AGTGTGAATGGCAGTGGACATTTTTGTGGAGTGGCTGAAATGAAGTCTGTTGTGGACTATAATGCGTAT GCTGGTGTCTGGTCTCAGGATAAGTGGAAGGGCAAATTTGAAGTTAAATGGATCTTTGTCAAAGATGTT CCCAATAACCAATTACGGCATATTCGCTTAGAAAATAATGACAACAAACCGGTTACCAATTCAAGGGAC ACTCAAGAGGTACCCCTAGAAAAAGCTAAGCAAGTGCTTAAAATAATTGCTACTTTCAAGCATACCACC TCAATCTTTGATGACTTTGCACATTATGAAAAGCGTCAAGAAGAGGAGGAAGCCATGCGTAGGGAGAGA AATAGAAACAAACAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001277816
Insert Size	1605 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277816.1
RefSeq Size	5085 bp
RefSeq ORF	1605 bp
Locus ID	253943
UniProt ID	Q7Z739
MW	58.3 kDa
Gene Summary	This gene encodes a member of the YTH (YT521-B homology) domain protein family. The YTH domain is common in eukaryotes, is often found in the middle of the protein sequence, and may function in binding to RNA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013] Transcript Variant: This variant (5) differs in its 5' UTR and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Variants 4, 5, 6, and 7 encode the same isoform.

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