CATSPER2 (NM_001282310) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336662 representing NM_001282310. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTCCTGGGCTCAGACATGGCCGCTTACCAACAAGAAGAGCAGATGCAGCTTCCCCGAGCTGATGCC ATTCGTTCACGTCTCATCGATACTTTCTCTCTCATTGAGCATTTGCAAGGCTTGAGCCAAGCTGTGCCG CGGCACACTATCAGGGAGTTACTTGATCCTTCCCGCCAGAAGAAACTTGTATTGGGAGATCAACACCAG CTAGTGCGTTTCTCTATAAAGCCTCAGCGTATAGAACAGATTTCACATGCCCAGAGGCTGTTGAGCAGG CTTCATGTGCGCTGCAGTCAGAGGCCACCTCTTTCTTTGTGGGCCGGATGGGTCCTTGAGTGTCCTCTC TTCAAAAACTTCATCATCTTCCTGGTCTTTTTGAATACGATCATATTGATGGTTGAAATAGAATTGCTG GAATCCACAAATACCAAACTATGGCCATTGAAGCTGACCTTGGAGGTGGCAGCTTGGTTTATCTTGCTT ATTTTCATCCTGGAGATCCTTCTTAAGTGGCTATCCAACTTTTCTGTTTTCTGGAAGAGTGCCTGGAAT GTCTTTGACTTTGTTGTTACCATGTTGTCCCTGCTTCCCGAGGTTGTGGTATTGGTAGGGGTAACAGGC CAATCGGTGTGGCTTCAGCTTCTGAGGATCTGCCGGGTGCTGAGGTCTCTCAAACTCCTTGCACAATTC CGTCAAATTCAAATTATTATTTTGGTCCTGGTCAGGGCCCTCAAGAGCATGACCTTCCTCTTGATGTTG CTGCTCATCTTCTTCTACATTTTTGCTGTGACTGGTGTCTACGTCTTCTCAGAGTACACCCGTTCACCT CGTCAGGACCTGGAGTACCATGTGTTCTTCTCGGACCTCCCGAATTCCCTGGTAACAGTGTTCATTCTC TTCACCTTGGATCATTGGTATGCACTGCTTCAGGACGTCTGGAAGGTGCCTGAAGTCAGTCGCATCTTC AGCAGCATCTATTTCATCCTTTGGTTGTTGCTTGGCTCCATTATCTTTCGAAGTATCATAGTAGCCATG ATGGTTACTAACTTTCAGAATATCAGGAAAGAGCTGAATGAGGAGATGGCGCGTCGGGAGGTTCAGCTC AAAGCTGACATGTTCAAGCGGCAGATCATCCAGAGGAGAAAAAACATGTCACATGAAGCACTGACGTCA AGCCATAGCAAAATAGAGGACAGAGGAGCTAGTCAACAAAGGGAAAGTTTGGACTTATCAGAAGTGTCT GAAGTAGAGTCTAATTATGGTGCCACTGAAGAGGATTTAATAACATCTGCATCAAAAACAGAAGAGACC TTGTCAAAAAAGAGAGAGTACCAGTCTTCCTCCTGTGTCTCCTCCACATCCTCTTCCTATTCTTCCTCT TCTGAATCCAGATTTTCTGAATCTATTGGTCGTTTGGACTGGGAGACTCTTGTGCACGAAAATCTGCCC GGGCTAATGGAAATGGATCAGGATGACCGTGTTTGGCCCAGAGACTCACTCTTCCGATATTTTGAGTTG CTAGAAAAGCTTCAGTATAACCTAGAGGAACGTAAGAAGTTACAAGAGTTTGCAGTGCAGGCACTGATG AACTTGGAAGACAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282310
Insert Size	1605 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282310.1
RefSeq Size	1755 bp
RefSeq ORF	1605 bp
Locus ID	117155
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
MW	62.5 kDa
Gene Summary	This gene encodes a member of a family of cation channel proteins that localize to the flagellum of spermatozoa. Defects at this locus causes male infertility. Alternatively spliced transcript variants have been observed at this locus. Readthrough transcription originates upstream of this locus in diphosphoinositol pentakisphosphate kinase 1 pseudogene 1 and is represented by GeneID:110006325. Related pseudogenes are found next to this locus on chromosome 15 and on chromosome 5. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (5) differs in the 5' region and initiates translation at an alternate start codon, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. The encoded isoform (5) has a distinct N-terminus and is longer than isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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