PTPN5 (NM_001278238) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PTPSTEP; STEP; STEP61
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336703 representing NM_001278238. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGCTGCAGTGAGAGGCTACCGGGTCTCCCCCAGCCGATAGTGATGGAGGCACTGGACGAGGCTGAA GGGCTCCAGGACTCACAGAGAGAGATGCCGCCACCCCCTCCTCCCTCGCCGCCCTCAGATCCAGCTCAG AAGCCACCACCTCGAGGCGCTGGGAGCCACTCCCTCACTGTCAGGAGCAGCCTGTGCCTGTTCGCTGCC TCACAGTTCCTGCTTGCCTGTGGGGTGCTCTGGTTCAGCGGTTATGGCCACATCTGGTCACAGAACGCC ACAAACCTCGTCTCCTCTTTGCTGACGCTCCTGAAACAGCTGGAACCCACGGCCTGGCTTGACTCTGGG ACGTGGGGAGTCCCCAGTCTGCTGCTGGTCTTTCTGTCCGTGGGCCTGGTCCTCGTTACCACCCTGGTG TGGCACCTCCTGAGGACACCCCCAGAGCCACCCACCCCACTGCCCCCTGAGGACAGGCGCCAGTCAGTG AGCCGCCAGCCCTCCTTCACCTACTCAGAGTGGATGGAGGAGAAGATCGAGGATGACTTCCTGGACCTC GACCCGGTGCCCGAGACTCCTGTGTTTGATTGTGTGATGGACATCAAGCCTGAGGCTGACCCCACCTCA CTCACCGTCAAGTCCATGGGTCTGCAGGAGAGGAGGGGTTCCAATGTCTCCCTGACCCTGGACATGTGC ACTCCGGGCTGCAACGAGGAGGGCTTTGGCTATCTCATGTCCCCACGTGAGGAGTCCGCCCGCGAGTAC CTGCTCAGCGCCTCCCGTGTCCTCCAAGCAGAAGAGCTTCATGAAAAGGCCCTGGACCCTTTCCTGCTG CAGGCGGAATTCTTTGAAATCCCCATGAACTTTGTGGATCCGAAAGAGTACGACATCCCTGGGCTGGTG CGGAAGAACCGGTACAAAACCATACTTCCCAACCCTCACAGCAGAGTGTGTCTGACCTCACCAGACCCT GACGACCCTCTGAGTTCCTACATCAATGCCAACTACATCCGGGGCTATGGTGGGGAGGAGAAGGTGTAC ATCGCCACTCAGGGACCCATCGTCAGCACGGTCGCCGACTTCTGGCGCATGGTGTGGCAGGAGCACACG CCCATCATTGTCATGATCACCAACATCGAGGAGATGAACGAGAAATGCACCGAGTATTGGCCGGAGGAG CAGGTGGCGTACGACGGTGTTGAGATCACTGTGCAGAAAGTCATTCACACGGAGGATTACCGGCTGCGA CTCATCTCCCTCAAGAGTGGGACTGAGGAGCGAGGCCTGAAGCATTACTGGTTCACATCCTGGCCCGAC CAGAAGACCCCAGACCGGGCCCCCCCACTCCTGCACCTGGTGCGGGAGGTGGAGGAGGCAGCCCAGCAG GAGGGGCCCCACTGTGCCCCCATCATCGTCCACTGCAGTGCAGGGATTGGGAGGACCGGCTGCTTCATT GCCACCAGCATCTGCTGCCAGCAGCTGCGGCAGGAGGGTGTGGTGGACATCCTGAAGACCACGTGCCAG CTCCGTCAGGACAGGGGCGGCATGATCCAGACATGCGAGCAGTACCAGTTTGTGCACCACGTCATGAGC CTCTACGAAAAGCAGCTGTCCCACCAGTCCCCAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278238
Insert Size	1626 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278238.1
RefSeq Size	3068 bp
RefSeq ORF	1626 bp
Locus ID	84867
UniProt ID	P54829
Protein Families	Druggable Genome, Phosphatase, Transmembrane
Protein Pathways	MAPK signaling pathway
MW	61 kDa
Gene Summary	May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) lacks an internal exon in the 5' region which causes translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (c) has a shorter N-teminus, compared to isoform a.

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