MUC20 (NM_020790) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MUC-20
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336774 representing NM_020790. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCTGTCTCTGGGGTCTGGCTCTGCCCCTTTTCTTCTTCTGCTGGGAGGTTGGGGTCTCTGGGAGC TCTGCAGGCCCCAGCACCCGCAGAGCAGACACTGCGATGACAACGGACGACACAGAAGTGCCCGCTATG ACTCTAGCACCGGGCCACGCCGCTCTGGAAACTCAAACACTGAGCGCTGAGACCTCTTCTAGGGCCTCA ACCCCAGCCGGCCCCGTTCCAGAAGCAGAGACCAGGGGATCCAAGAGAATTTCCCCTGCAAGAGAGACC AGGAGTTTCACAAAAACATCTCCCAACTTCATGGTGCTGATCGCCACCTCCGTGGAGACATCAGCCGCC AGTGGCAGCCCCGAGGGAGCTGGAATGACCACAGTTCAGACCATCACAGGCAGTGATCCCGAGGAAGCC ATCTTTGACACCCTTTGCACCGATGACAGCTCTGAAGAGGCAAAGACACTCACAATGGACATATTGACA TTGGCTCACACCTCCACAGAAGCTAAGGGCCTGTCCTCAGAGAGCAGTGCCTCTTCCGACGGCCCCCAT CCAGTCATCACCCCGTCACGGGCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCACC CCGTCACGGGCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCACCCCGTCACGGGCC TCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCACCCCCTCATGGTCCCCGGGATCTGAC GTCACTCTCCTCGCTGAAGCCCTGGTGACTGTCACAAACATCGAGGTTATTAATTGCAGCATCACAGAA ATAGAAACAACGACTTCCAGCATCCCTGGGGCCTCAGACACAGATCCCATCCCCACGGAAGGGGTGAAG GCCTCGTCCACCTCCGATCCACCAGCTCTGCCTGACTCCACTGAAGCAAAACCACACATCACTGAGGTC ACAGCCTCTGCCGAGACCCTGTCCACAGCCGGCACCACAGAGTCAGCTGCACCTGATGCCACGATTGGG ACCCCACTCCCCACCAACAGCACCATAGAAAGAGAAGTGACAGCACCCGGGGCCACGACCCTCAGTGGA GCTCTGGCCACAGGGAATCCCCTGGAAGAAACCTCAGCCCTCTCTGTTGAGACACCAAGTTACGTCAAA GTCTCAGGAGCAGCTCCGGTCTCCATAGAGGCTGGGTCAGCAGTGGGCAAAACAACTTCCTTTGCTGGG AGCTCTGCTTCCTCCTACAGCCCCTCGGAAGCCGCCCTCAAGAACTTCACCCCTTCAGAGACACTGACC ACGGACATCGCAACCAAGGGGCCCTTCCCCACCAGCAGGGACCCTCTTCCTTCTGTCCCTCCAACTACA ACCAACAGCAGCCGAGGGACGAACAGCACCTTAGCCAAGATCACAACCTCAGCGAAGACCACGATGAAG CCCCCAACAGCCACGCCCACGACTGCCCGGACGAGGCCGACCACAGACATGAGTGCAGGTGAAAATGGA GGTTTCCTCCTCCTGCGGCTGAGTGTGGCTTCCCCGGAAGACCTCACTGACCCCAGAGTGGCAGAAAGG CTGATGCAGCAGCTCCACCGGGAACTCCACGCCCACGCGCCTCACTTCCAGGTCTCCTTACTGCGTGTC AGGAGAGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_020790
Insert Size	1668 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020790.1
RefSeq Size	2146 bp
RefSeq ORF	1668 bp
Locus ID	200958
UniProt ID	Q8N307
MW	56.7 kDa
Gene Summary	This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (4) lacks eight mucin tandem repeat sequences in the central coding region, and also has additional polymorphisms in the coding region and 5' UTR, compared to variant 1. The encoded isoform (d) is shorter than isoform a. This variant includes four mucin tandem repeats and is represented on the ALT_REF_LOCI_7 alternate haplotype of the reference genome.

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