RMC1 (NM_001276342) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C18orf8; HsT2591; Mic-1; MIC1; WDR98
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336793 representing NM_001276342. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCGAGGAGGACTACTATCTGGAGCTGTGCGAGCGGCCGGTGCAGTTCGAGAAGGCGAACCCTGTC AACTGCGTCTTCTTCGATGAGGCCAACAAGCAGGTTTTTGCTGTTCGATCTGGTGGAGCTACTGGCGTG GTAGTTAAAGGCCCAGATGATAGGAATCCCATCTCATTTAGAATGGATGACAAAGGAGAAGTGAAGTGC ATTAAGTTTTCCTTAGAAAATAAGATATTGGCTGTTCAGAGGACCTCAAAGACTGTGGTATTACCAGAG AAACGGAGTCTGAAACTCTTGAAGAGCCACAATCTCAATGTGAATTGGTACATGTACTGCCCCGAGAGC GCCGTGATCTTGCTGTCTACCACGGTCCTGGAGAATGTCCTGCAGCCTTTTCACTTTAGGGCTGGCACT ATGTCGAAGCTGCCCAAATTTGAGATTGAATTACCAGCTGCGCCTAAGTCAACTAAACCCAGCCTTTCC GAAAGAGACATCGCAATGGCTACCATATACGGGCAGCTGTATGTTCTCTTCTTGAGGCATCATTCTCGG ACCTCCAACAGCACAGGAGCGGAGGTGGTCCTCTATCATCTACCACGAGAAGGTGCCTGTAAAAAGATG CACATATTGAAGTTAAATAGGACGGGAAAGTTTGCCCTGAACGTGGTGGACAACCTGGTAGTCGTGCAT CATCAGGATACAGAGACATCGGTAATATTCGATATCAAGTTACGGGGAGAGTTTGACGGCTCCGTTACC TTCCACCACCCCGTGCTTCCCGCTCGATCGATCCAGCCCTATCAGATCCCCATCACAGGTCCTGCTGCC GTGACCAGCCAGTCTCCTGTTCCATGTAAACTCTATTCTTCATCTTGGATTGTCTTTCAACCTGACATC ATTATCAGCGCAAGCCAAGGTTACCTCTGGAACCTCCAAGTGAAACTTGAGCCCATAGTAAATCTCTTA CCAGACAAAGGAAGACTCATGGACTTTCTCCTCCAGAGAAAGGAATGCAAGATGGTCATCCTGTCTGTC TGTTCACAGATGTTAAGTGAGTCAGACAGAGCATCGCTGCCCGTGATAGCCACTGTTTTTGATAAACTC AACCATGAGTATAAAAAGTACCTGGATGCCGAGCAGAGTTATGCGATGGCGGTGGAAGCAGGGCAGAGC CGAAGCAGCCCGCTCCTCAAGAGGCCGGTGCGGACCCAGGCGGTGCTGGACCAGTCAGATGTGTACACC CATGTCCTGTCAGCCTTTGTGGAAAAGAAGGAGATGCCTCATAAATTTGTGATAGCCGTGCTGATGGAA TACATTCGTTCTCTTAACCAGTTTCAGATTGCAGTACAGCATTACCTACATGAACTTGTTATCAAAACC CTTGTCCAGCACAACCTCTTTTATATGCTGCATCAGTTCCTGCAGTACCACGTCCTCAGCGACTCCAAA CCTTTGGCTTGTCTGCTGTTATCCCTAGAGAGTTTCTATCCTCCTGCTCATCAGCTATCTCTGGACATG CTGAAGATGCTGCAAAGCAGACTGAAGACAACATGCTTTTCTATACAATATTCCGCTTTTTTGAACAGC GAAACCAGCGTTTGCGAGGGAGCCCCAATTTCACACCAGGGGAACACTGTGAAGAACATGTTGCTTTTT TCAAACAGATTTTTGGAGACCAAGCTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001276342
Insert Size	1686 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276342.1
RefSeq Size	1958 bp
RefSeq ORF	1686 bp
Locus ID	29919
MW	63.7 kDa
Gene Summary	This gene encodes a colon cancer associated protein. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 5' coding region and uses an alternate splice site in the 3' coding region, which results in a frameshift compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

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