HCE (RNGTT) (NM_001286426) Human Untagged Clone

CAT#: SC336841

RNGTT (untagged) - Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), transcript variant 2



  "NM_001286426" in other vectors (2)

CNY 5,610.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal antibody to HCE (RNA guanylyltransferase and 5'-phosphatase)
    • 100 ul

CNY 6,281.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CAP1A; hCAP; HCE; HCE1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC336841 representing NM_001286426.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTCACAACAAGATCCCGCCGCGGTGGCTGAACTGTCCCCGGCGCGGCCAGCCGGTGGCAGGAAGA
TTCTTACCTCTGAAGACAATGTTAGGACCAAGATATGATAGTCAAGTTGCTGAAGAAAATCGGTTCCAT
CCCAGCATGCTCTCAAATTACCTAAAGAGCCTAAAGGTTAAAATGGGCTTGTTGGTGGACCTGACAAAT
ACTTCAAGGTTCTATGACCGAAATGACATAGAAAAAGAAGGAATCAAATATATAAAACTTCAGTGTAAA
GGACATGGTGAGTGCCCTACCACTGAGAATACTGAGACCTTTATTCGTCTGTGTGAGCGGTTTAATGAA
AGAAATCCACCTGAACTTATAGGTGTTCATTGTACTCATGGCTTCAATCGCACTGGTTTCCTCATATGT
GCCTTTTTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCAGTTGCTACTTTTGCCCAAGCCAGACCA
CCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCGGTATGGTGACATAGAGGAAGCACCA
CCCCCACCTCTATTGCCAGATTGGTGTTTTGAGGATGATGAAGACGAAGATGAGGATGAGGATGGAAAG
AAGGAATCAGAACCCGGGTCAAGTGCTTCTTTTGGCAAAAGGAGAAAAGAACGGTTAAAACTGGGCGCT
ATTTTCTTGGAAGGTGTTACTGTTAAAGGTGTAACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTA
CAGCAGAAGTGTCATCAATTCTGTGGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATG
GACAAGCAAAATATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGG
TACATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTTCATGTT
TCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACTCTCTTGGATGGCGAG
ATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTGATATATGACATAATTAAATTCAAT
TCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGTCTGCAGTGTATAGAACGAGAAATTATAAGTCCT
CGACACGAAAAAATGAAGACTGGGCTCATTGACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCG
TTTTTTGACATCTGTACTTCAAGAAAGAAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAG
CCTCCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGGGTTACTTCCT
CAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAAATCAAGGTGACAAAAGAG
CTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAGAACAACAGCTGGGTCTTCATGAGACAG
AGAACAGACAAAAGTTTTCCTAATGCCTACAACACTGCCATGGCTGTGTGTAACAGCATCTCAAACCCT
GTCACCAAGGAGATGCTGTTTGAGTTCATCGACAGATGTACTGCAGCTTCTCAAGGACAGAAGCGAAAA
CATCATCTGGACCCTGACACGGAGCTCATGCCACCACCACCTCCCAAAAGACCACGCCCTTTAACCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001286426
Insert Size 1725 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001286426.1
RefSeq Size 4772 bp
RefSeq ORF 1725 bp
Locus ID 8732
UniProt ID O60942
Protein Families Druggable Genome, Phosphatase
MW 66 kDa
Gene Summary Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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