DNA polymerase eta (POLH) (NM_001291969) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RAD30; RAD30A; XP-V; XPV
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336898 representing NM_001291969. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGCTCGTGCATTTGGAGTCACTAGAAGTATGTGGGCAGATGATGCTAAGAAGTTATGTCCAGATC TTCTACTGGCACAAGTTCGTGAGTCCCGTGGGAAAGCTAACCTCACCAAAGGGGATGCGAAAACAAGGC TTATTTCAATGGCTCGATTCTCTTCAGATTGATAACCTCACCTCTCCAGACCTGCAGCTCACCGTGGGA GCAGTGATTGTGGAGGAAATGAGAGCAGCCATAGAGAGGGAGACTGGTTTTCAGTGTTCAGCTGGAATT TCACACAATAAGGTCCTGGCAAAACTGGCCTGTGGACTAAACAAGCCCAACCGCCAAACCCTGGTTTCA CATGGGTCAGTCCCACAGCTCTTCAGCCAAATGCCCATTCGCAAAATCCGTAGTCTTGGAGGAAAGCTA GGGGCCTCTGTCATTGAGATCCTAGGGATAGAATACATGGGTGAACTGACCCAGTTCACTGAATCCCAG CTCCAGAGTCATTTTGGGGAGAAGAATGGGTCTTGGCTATATGCCATGTGCCGAGGGATTGAACATGAT CCAGTTAAACCCAGGCAACTACCCAAAACCATTGGCTGTAGTAAGAACTTCCCAGGAAAAACAGCTCTT GCTACTCGGGAACAGGTACAATGGTGGCTGTTGCAATTAGCCCAGGAACTAGAGGAGAGACTGACTAAA GACCGAAATGATAATGACAGGGTAGCCACCCAGCTGGTTGTGAGCATTCGTGTACAAGGAGACAAACGC CTCAGCAGCCTGCGCCGCTGCTGTGCCCTTACCCGCTATGATGCTCACAAGATGAGCCATGATGCATTT ACTGTCATCAAGAACTGTAATACTTCTGGAATCCAGACAGAATGGTCTCCTCCTCTCACAATGCTTTTC CTCTGTGCTACAAAATTTTCTGCCTCTGCCCCTTCATCTTCTACAGACATCACCAGCTTCTTGAGCAGT GACCCAAGTTCTCTGCCAAAGGTGCCAGTTACCAGCTCAGAAGCTAAGACCCAGGGAAGTGGCCCAGCG GTGACAGCCACTAAGAAAGCAACCACGTCTCTGGAATCATTCTTCCAAAAAGCTGCAGAAAGGCAGAAA GTTAAAGAAGCTTCGCTTTCATCTCTTACTGCTCCCACTCAGGCTCCCATGAGCAATTCACCATCCAAG CCCTCATTACCTTTTCAAACCAGTCAAAGTACAGGAACTGAGCCCTTCTTTAAGCAGAAAAGTCTGCTT CTAAAGCAGAAACAGCTTAATAATTCTTCAGTTTCTTCCCCCCAACAAAACCCATGGTCCAACTGTAAA GCATTACCAAACTCTTTACCAACAGAGTATCCAGGGTGTGTCCCTGTTTGTGAAGGGGTGTCGAAGCTA GAAGAATCCTCTAAAGCAACTCCTGCAGAGATGGATTTGGCCCACAACAGCCAAAGCATGCACGCCTCT TCAGCTTCCAAATCTGTGCTGGAGGTGACTCAGAAAGCAACCCCAAATCCAAGTCTTCTAGCTGCTGAG GACCAAGTGCCCTGTGAGAAGTGTGGCTCCCTGGTACCGGTATGGGATATGCCAGAACACATGGACTAT CATTTTGCATTGGAGTTGCAGAAATCCTTTTTGCAGCCCCACTCTTCAAACCCCCAGGTTGTTTCTGCC GTATCTCATCAAGGCAAAAGAAATCCCAAGAGCCCTTTGGCCTGCACTAATAAACGCCCCAGGCCTGAG GGCATGCAAACATTGGAATCATTTTTTAAGCCATTAACACATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291969
Insert Size	1770 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291969.1
RefSeq Size	8053 bp
RefSeq ORF	1770 bp
Locus ID	5429
Protein Families	Druggable Genome
MW	64.8 kDa
Gene Summary	This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) lacks two alternate exons compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.