ZNF274 (NM_001278734) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HFB101; ZF2; ZKSCAN19; ZSCAN51
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336973 representing NM_001278734. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGGAGAACTACAGGAACCTGGTCTCAGTGGAACATCAGCTTTCCAAACCAGATGTGGTATCTCAG TTAGAGGAGGCAGAAGATTTCTGGCCAGTGGAGAGAGGAATTCCTCAAGACACCATTCCAGAGTATCCT GAGCTCCAGCTGGACCCTAAATTGGATCCTCTTCCTGCTGAGAGTCCCCTAATGAACATTGAGGTTGTT GAGGTCCTCACACTGAACCAGGAGGTGGCTGGTCCCCGGAATGCCCAGATCCAGGCCCTATATGCTGAA GATGGAAGCCTGAGTGCAGATGCCCCCAGTGAGCAGGTCCAACAGCAGGGCAAGCATCCAGGTGACCCT GAGGCCGCGCGCCAGAGGTTCCGGCAGTTCCGTTATAAGGACATGACAGGTCCCCGGGAGGCCCTGGAC CAGCTCCGAGAGCTGTGTCACCAGTGGCTACAGCCTAAGGCACGCTCCAAGGAGCAGATCCTGGAGCTG CTGGTGCTGGAGCAGTTCCTAGGTGCACTGCCTGTGAAGCTCCGGACATGGGTGGAATCGCAGCACCCA GAGAACTGCCAAGAGGTGGTGGCCCTGGTAGAGGGTGTGACCTGGATGTCTGAGGAGGAAGTACTTCCT GCAGGACAACCTGCCGAGGGCACCACCTGCTGCCTCGAGGTCACTGCCCAGCAGGAGGAGAAGCAGGAG GATGCAGCCATCTGCCCAGTGACAGTGCTCCCTGAGGAGCCAGTGACCTTCCAGGATGTGGCTGTGGAC TTCAGCCGGGAGGAGTGGGGGCTGCTGGGCCCGACACAGAGGACCGAGTACCGCGATGTGATGCTGGAG ACCTTTGGGCACCTGGTCTCTGTGGGGTGGGAGACTACACTGGAAAATAAAGAGTTAGCTCCAAATTCT GACATTCCTGAGGAAGAACCAGCCCCCAGCCTGAAAGTACAAGAATCCTCAAGGGATTGTGCCTTGTCC TCTACATTAGAAGATACCTTGCAGGGTGGGGTCCAGGAAGTCCAAGACACAGTGTTGAAGCAGATGGAG TCTGCTCAGGAAAAAGACCTTCCTCAGAAGAAGCACTTTGACAACCGTGAGTCCCAGGCAAACAGTGGT GCTCTTGACACAAACCAAGTTTCGCTCCAGAAAATTGACAACCCTGAGTCCCAGGCAAACAGTGGCGCT CTTGACACAAACCAAGTTTTGCTCCACAAAATTCCTCCTAGAAAACGATTGCGCAAACGTGACTCACAA GTTAAAAGTATGAAACATAATTCACGTGTAAAAATTCATCAGAAGAGCTGTGAAAGGCAAAAGGCCAAG GAAGGCAATGGTTGTAGGAAAACCTTCAGTCGGAGTACTAAACAGATTACGTTTATAAGAATTCACAAG GGGAGCCAAGTTTGCCGATGCAGTGAATGTGGTAAAATATTCCGGAACCCAAGATACTTTTCTGTGCAT AAGAAAATCCATACCGGAGAGAGGCCCTATGTGTGTCAAGACTGTGGGAAAGGATTTGTTCAGAGCTCT TCCCTCACACAGCATCAGAGAGTTCATTCTGGAGAGAGACCATTTGAATGTCAGGAGTGTGGGAGGACC TTCAATGATCGCTCAGCCATCTCCCAGCACCTGAGGACTCACACTGGCGCTAAGCCCTACAAGTGTCAG GACTGTGGAAAAGCCTTCCGCCAGAGCTCCCACCTCATCAGACATCAGAGGACTCACACCGGGGAGCGC CCATATGCATGCAACAAATGTGGAAAGGCCTTCACCCAGAGCTCACACCTTATTGGGCACCAGAGAACC CACAATAGGACAAAGCGAAAGAAGAAACAGCCTACCTCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278734
Insert Size	1836 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278734.1
RefSeq Size	2905 bp
RefSeq ORF	1836 bp
Locus ID	10782
UniProt ID	Q96GC6
Protein Families	Transcription Factors
Protein Pathways	Neurotrophin signaling pathway
MW	69.4 kDa
Gene Summary	This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (ZNF274d) has an additional segment in the 5' region, which results in a downstream translation start codon, compared to variant ZNF274c. The resulting isoform (d) has a shorter N-terminus and contains only one KRAB A domain, compared to isoform c.

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