HIP1R (NM_001303097) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HIP3; HIP12; ILWEQ
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337000 representing NM_001303097. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACAGCATCAAGAACGTGCCGGCGCGGGTGCTGAGCCGCAGGCCGGGCCACAGCCTGGAGGCCGAG CGCGAGCAGTTCGACAAGACCCAGGCCATCAGCATCAGCAAAGCCATCAACACCCAGGAGGCCCCCGTG AAGGAGAAGCACGCCCGGCGCATCATTCTGGGCACACACCACGAGAAGGGGGCTTTCACCTTCTGGTCC TACGCCATTGGGCTGCCGCTGCCCAGCAGCTCCATTCTCAGCTGGAAGTTCTGCCACGTCCTCCACAAG GTCCTTCGAGACGGGCACCCCAATGTGCTGCATGACTGCCAGCGGTACCGCAGCAACATCCGGGAGATT GGAGACCTGTGGGGACATTTGCATGACCGCTACGGACAGCTGGTGAATGTCTACACCAAGCTGCTGCTG ACCAAGATCTCCTTCCACCTCAAGCATCCCCAGTTTCCCGCGGGCCTGGAGGTGACAGATGAGGTACTG GAGAAGGCAGCTGGGACCGATGTCAACAACATCTTCCAGCTCACTGTGGAGATGTTTGATTACATGGAT TGTGAGCTGAAGCTTTCTGAATCAGTTTTCCGACAGCTCAACACGGCCATCGCCGTATCCCAGATGTCC TCAGGCCAGTGCCGCCTGGCCCCCCTCATCCAGGTCATCCAGGACTGCAGCCACCTCTACCACTACACG GTCAAGCTCCTGTTCAAGCTACACTCTTGTCTCCCTGCGGACACCCTGCAAGGCCACAGGGACCGGTTC CACGAGCAGTTTCACAGCCTCAGGAACTTCTTCCGCAGAGCCTCCGACATGCTGTACTTCAAGCGGCTC ATCCAGATCCCCCGGCTGCCCGAGGGACCCCCTAACTTCCTGCGGGCCTCAGCCCTGGCTGAGCACATC AAGCCGGTGGTGGTGATCCCCGAGGAGGCCCCGGAAGATGAGGAGCCGGAGAATCTCATTGAGATCAGC ACAGGGCCCCCCGCGGGGGAGCCAGTGGTGGTGGCTGACCTCTTCGATCAGACGTTTGGACCCCCCAAT GGGTCTGTGAAGGACGACAGGGACCTCCAGATTGAGAGCTTGAAGAGAGAGGTGGAAATGCTCCGCTCT GAACTGGAGAAGATCAAGCTGGAGGCCCAGCGGTACATCGCGCAGCTGAAGAGCCAGGTGAATGCACTG GAGGGTGAGCTGGAGGAGCAGCGGAAGCAGAAGCAGAAGGCCCTGGTGGATAATGAGCAGCTCCGCCAC GAGCTGGCCCAGCTGAGGGCTGCCCAGCTGGAGGGCGAGCGGAGCCAGGGCCTGCGTGAGGAGGCTGAG AGGAAGGCCAGTGCCACGGAGGCGCGCTACAACAAGCTGAAGGAAAAGCACAGTGAGCTCGTCCATGTG CACGCGGAGCTGCTCAGAAAGAACGCGGACACAGCCAAGCAGCTGACGGTGACGCAGCAAAGCCAGGAG GAGGTGGCGCGGGTGAAGGAGCAGCTGGCCTTCCAGGTGGAGCAGGTGAAGCGGGAGTCGGAGTTGAAG CTAGAGGAGAAGAGCGACCAGCTGGAGAAGCTCAAGAGGGAGCTGGAGGCCAAGGCCGGAGAGCTGGCC CGCGCGCAGGAGGCCCTGAGCCACACAGAGCAGAGCAAGTCGGAGCTGAGCTCACGGCTGGACACGCTG AGTGCGGAGAAGGATGCTCTGAGTGGAGCTGTGCGGCAGCGGGAGGCAGACCTGCTGGCGGCGCAGAGC CTGGTGCGCGAGACAGAGGCGGCGCTGAGCCGGGAGCAGCAGCGCAGCTCCCAGGAGCAGGGCGAGTTG CAGGGCCGGCTGGCAGAGAGGGTATGGCCTCCCCAGATGCAGCAGCACCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303097
Insert Size	1848 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303097.1
RefSeq Size	2613 bp
RefSeq ORF	1848 bp
Locus ID	9026
UniProt ID	O75146
MW	70.3 kDa
Gene Summary	Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks several 3' exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.

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