TLE4 (NM_001282760) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCE-1; BCE1; E(spI); E(spl); ESG; ESG4; Grg-4; GRG4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337103 representing NM_001282760. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGAACTGAACGCCATCATTGGGCAACAACTCCAGGCCCAGCATTTATCACATGGACATGGTCTC CCCGTACCTCTGACTCCACACCCTTCAGGGCTCCAGCCCCCTGCCATTCCACCCATCGGTAGCAGTGCC GGGCTTCTGGCCCTCTCCAGTGCTCTAGGAGGTCAGTCCCATCTTCCAATTAAAGATGAGAAGAAGCAC CATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCAGTATCCCCATCAGCCAGTTTC CGAGGTGCTGAGAAGCACAGAAACTCCGCAGACTACTCCTCAGAGAGCAAAAAGCAGAAAACTGAAGAA AAGGAAATTGCAGCTCGTTATGACAGCGATGGTGAGAAAAGTGATGACAACTTGGTGGTTGACGTTTCC AATGAGGATCCATCTTCCCCTCGAGGGAGCCCAGCACATTCCCCCAGAGAGAATGGCCTAGACAAGACA CGCCTGCTCAAGAAAGATGCCCCGATTAGTCCAGCCTCTATTGCATCTTCCAGCAGTACTCCCTCCTCC AAATCCAAAGAACTTAGCCTTAATGAAAAATCTACTACTCCCGTCTCAAAGTCCAATACCCCTACTCCA CGAACTGATGCGCCCACCCCAGGCAGTAACTCTACTCCCGGATTGAGGCCTGTACCTGGAAAACCACCA GGAGTTGACCCTTTGGCCTCAAGCCTAAGGACCCCAATGGCAGTACCTTGTCCATATCCAACTCCATTT GGGATTGTGCCCCATGCTGGAATGAACGGAGAGCTGACCAGCCCCGGAGCGGCCTACGCTGGGCTCCAC AACATCTCCCCTCAGATGAGCGCAGCTGCTGCCGCCGCCGCTGCTGCTGCTGCCTATGGGAGATCACCA GTGGTGGGATTTGATCCACACCATCACATGCGTGTGCCAGCAATACCTCCAAACCTGACAGGCATTCCA GGAGGAAAACCAGCATACTCCTTCCATGTTAGCGCAGATGGTCAGATGCAGCCTGTCCCTTTTCCACCC GACGCCCTCATCGGACCTGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTG GTGTGCGCGGTGACCATCAGCAACCCCACGAGACACGTGTACACGGGTGGGAAGGGCTGCGTCAAGGTC TGGGACATCAGCCACCCAGGCAATAAGAGTCCTGTCTCCCAGCTCGACTGTCTGAACAGGGATAACTAC ATCCGTTCCTGCAGATTGCTCCCTGATGGTCGCACCCTAATTGTTGGAGGGGAAGCCAGTACTTTGTCC ATTTGGGACCTGGCGGCTCCAACCCCACGCATCAAGGCAGAGCTGACATCCTCGGCCCCCGCCTGCTAT GCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGG GATCTGCACAACCAGACCTTGGTGAGGCAATTCCAGGGCCACACAGATGGAGCCAGCTGTATTGACATT TCTAATGATGGCACCAAGCTCTGGACAGGTGGTTTGGACAACACGGTCAGGTCCTGGGACCTGCGCGAG GGGCGGCAGCTGCAGCAGCACGACTTCACCTCCCAGATCTTTTCTCTGGGCTACTGCCCAACTGGAGAG TGGCTTGCAGTGGGGATGGAGAACAGCAATGTGGAAGTTTTGCATGTCACCAAGCCAGACAAATACCAA CTACATCTTCATGAGAGCTGTGTGCTGTCGCTCAAGTTTGCCCATTGTGGCAAATGGTTTGTAAGCACT GGAAAGGACAACCTTCTGAATGCCTGGAGAACACCTTATGGGGCCAGTATATTCCAGTCCAAAGAATCC TCATCGGTGCTTAGCTGTGACATCTCCGTGGACGACAAATACATTGTCACTGGCTCTGGGGATAAGAAG GCCACAGTTTATGAAGTTATTTATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282760
Insert Size	1959 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282760.1
RefSeq Size	5173 bp
RefSeq ORF	1959 bp
Locus ID	7091
Protein Families	Transcription Factors
MW	69.4 kDa
Gene Summary	Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) uses an alternate exon in the 5' UTR and lacks an alternate in-frame exon in the coding region which results in the use of a downstream start codon compared to variant 1. It encodes isoform 5 which has a shorter N-terminus compared the isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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