PHEX (NM_001282754) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HPDR; HPDR1; HYP; HYP1; LXHR; PEX; XLH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282754, the custom clone sequence may differ by one or more nucleotides ATGGAAGCAGAAACAGGGAGCAGCGTGGAGACTGGAAAGAAGGCCAACAGAGGCACTCGAATTGCCCTGG TCGTGTTTGTCGGTGGCACCCTAGTTCTGGGCACGATCCTCTTTCTAGTGAGTCAAGGTCTCTTAAGTCT CCAAGCTAAACAGGAGTACTGCCTGAAGCCAGAATGCATCGAAGCGGCTGCTGCCATCTTAAGTAAAGTA AATCTGTCTGTGGATCCTTGTGATAATTTCTTCCGGTTCGCTTGTGATGGCTGGATAAGCAATAATCCAA TTCCCGAAGATATGCCAAGCTATGGGGTTTATCCTTGGCTGAGACATAATGTTGACCTCAAGTTGAAGGA ACTTTTGGAGAAATCAATCAGTAGAAGGCGGGACACCGAAGCCATACAGAAAGCCAAAATCCTTTATTCA TCCTGCATGAATGAGAAAGCGATTGAAAAAGCAGATGCCAAGCCACTGCTACACATCCTACGGCATTCAC CTTTCCGCTGGCCCGTGCTTGAATCTAATATTGGCCCTGAAGGGGTTTGGTCAGAGAGAAAGTTCAGCCT TCTGCAGACACTTGCAACGTTTCGTGGTCAATACAGCAATTCTGTGTTCATCCGTTTGTATGTGTCCCCT GATGACAAAGCATCCAATGAACATATCTTGAAGCTGGACCAAGCAACACTCTCCCTGGCCGTGAGGGAAG ACTACCTTGATAACAGTACAGAAGCCAAGTCTTATCGGGATGCCCTTTACAAGTTCATGGTGGATACTGC CGTGCTTTTAGGAGCTAACAGTTCCAGAGCAGAGCATGACATGAAGTCAGTGCTCAGATTGGAAATTAAG ATAGCTGAGATAATGATTCCACATGAAAACCGAACCAGCGAGGCCATGTACAACAAAATGAACATTTCTG AACTGAGTGCTATGATTCCCCAGTTCGACTGGCTGGGCTACATCAAGAAGGTCATTGACACCAGACTCTA CCCCCATCTGAAAGACATCAGCCCCTCCGAGAATGTGGTGGTCCGCGTCCCGCAGTACTTTAAAGATTTG TTTAGGATATTAGGGTCTGAGAGAAAGAAGACCATTGCCAACTATTTGGTGTGGAGAATGGTTTATTCCA GAATTCCAAACCTTAGCAGGCGCTTTCAGTATAGATGGCTGGAATTCTCAAGGGTAATCCAGGGGACCAC AACTTTGCTGCCTCAATGGGACAAATGTGTAAACTTTATTGAAAGTGCCCTCCCTTATGTTGTTGGAAAG ATGTTTGTAGATGTGTACTTCCAGGAAGATAAGAAGGAAATGATGGAGGAATTGGTTGAGGGCGTTCGCT GGGCCTTTATTGACATGCTAGAGAAAGAAAATGAGTGGATGGATGCAGGAACGAAAAGGAAAGCCAAAGA AAAGGCGAGAGCTGTTTTGGCAAAAGTTGGCTATCCAGAGTTTATAATGAATGATACTCATGTTAATGAA GACCTCAAAGCTATCAAGTTTTCAGAAGCCGACTACTTTGGCAACGTCCTACAAACTCGCAAGTATTTAG CACAGTCTGATTTCTTCTGGCTAAGAAAAGCCGTTCCAAAAACAGAGTGGTTTACAAATCCGACGACTGT CAATGCCTTCTACAGTGCATCCACCAACCAGATCCGATTTCCAGCAGGAGAGCTCCAGAAGCCTTTCTTT TGGGGAACAGAATATCCTCGATCTCTGAGTTATGGTGCTATAGGAGTAATTGTCGGACATGAATTTACAC ATGGATTTGATAATAATGGTAGAAAATATGATAAAAATGGAAACCTGGATCCTTGGTGGTCTACTGAATC AGAAGAAAAGTTTAAGGAAAAAACAAAATGCATGATTAACCAGTATAGCAACTATTATTGGAAGAAAGCT GGCTTAAATGTCAAGGGGAAGAGGACCCTGGGAGAAAATATTGCTGATAATGGAGGCCTGCGGGAAGCTT TTAGGGCTTACAGGAAATGGATAAATGACAGAAGGCAGGGACTTGAGGAGCCTCTTCTACCAGGCATCAC ATTCACCAACAACCAGCTCTTCTTCCTGAGTTATGCTCATGGTCAATGGTGCAATTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001282754
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282754.1, NP_001269683.1
RefSeq Size	6092 bp
RefSeq ORF	2088 bp
Locus ID	5251
UniProt ID	P78562
Protein Families	Druggable Genome, Protease, Transmembrane
Gene Summary	The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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