CDRT1 (NM_001282540) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C17ORF1; C17ORF1A; C170RF1; FBXW10B; FBXW10P1; HREP; SM25H2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282540, the custom clone sequence may differ by one or more nucleotides ATGGAAAACCTGGAATCAAGGCTCAAGAATGCCCCCTATTTTCGTTGTGAGAAGGGAACCGATTCCATCC CTCTATGCCGGAAGTGTGAGACGCGTGTCTTAGCCTGGAAGATCTTCTCTACCAAAGAGTGGTTCTGCAG GATCAATGACATATCACAGAGGAGGTTTCTAGTTGGCATTCTGAAGCAGTTAAATAGCTTATATTTGTTA CACTATTTCCAAAATATCCTTCAGACCACACAGGGAAAGGATTTCATCTATAACAGGTCCCGGATCGACC TCAGCAAGAAAGAGGGGAAAGTTGTGAAGTCCTCCTTGAACCAAATGTTGGATAAAACAGTAGAACAGAA GATGAAAGAGATCTTGTATTGGTTTGCGAACAGCACCCAGTGGACCAAGGCGAATTATACTCTCTTACTG CTGCAGATGTGCAACCCCAAATTACTGCTCACTGCTGCCAATGTGATCAGAGTCCTGTTTCTGAGAGAGG AGAACAATATCTCAGGGCTCAATCAAGACATCACAGATGTGTGTTTTTCCCCTGAGAAAGACCACAGCTC CAAGTCTGCGACCTCACAAGTCTATTGGACAGCCAAAACTCAGCACACATCCCTTCCTTTGTCCAAAGCC CCAGAAAATGAACACTTCCTTGGGGCAGCATCTAACCCTGAGGAACCATGGAGGAATTCACTCCGGTGTA TATCCGAAATGAATAGGCTGTTTTCTGGAAAAGCAGACATAACCAAGCCAGGGTACGATCCCTGCAATCT ATTGGTTGACCTGGATGACATCAGAGACCTGTCTTCTGGGTTCAGCAAATACCGAGACTTCATCCGTTAC CTGCCCATCCACCTCTCCAAGTACATTCTAAGAATGCTGGATAGACACACCCTGAACAAGTGCGCCTCTG TGAGCCAGCACTGGGCCGCCATGGCTCAACAGGTCAAGATGGACTTGTCAGCGCACGGCTTCATTCAGAA CCAGATTACCTTCTTGCAGGGGTCCTATACAAGAGGAATTGATCCTAATTATGCCAATAAGGTTTCTATC CCAGTTCCTAAAATGGTAGATGACGGGAAGAGCATGCGTGTGAAACATCCGAAGTGGAAGTTGAGAACGA AGAATGAGTACAACCTGTGGACTGCATACCAGAACGAGGAAACGCAGCAGGTCCTGATGGAGGAGAGAAA TGTTTTCTGTGGGACCTACAATGTTCGCATTCTCTCTGACACGTGGGATCAAAACCGAGTCATCCACTAT TCCGGGGGAGATCTGATAGCTGTGTCATCTAATCGAAAGATCCATCTTCTGGACATCATACAAGTGAAAG CGATACCCGTTGAATTCCGAGGCCATGCTGGGAGTGTCCGGGCCCTCTTCCTGTGTGAGGAGGAAAACTT TCTCCTAAGCGGGAGCTATGACCTAAGTATCAGATACTGGGATCTGAAAAGTGGGGTTTGCACACGAATC TTCGGTGGTCACCAGGGGACTATCACTTGCATGGACTTGTGTAAGAACAGGCTCGTATCTGGAGGAAGAG ATTGCCAGGTAAAAGTATGGGATGTAGACACAGGGAAGTGCCTGAAGACGTTTAGACACAAAGACCCCAT CTTGGCCACCAGGATCAATGATACCTACATTGTGAGCAGCTGTGAGCGAGGGCTGGTGAAAGTGTGGCAC ATTGCCATGGCCCAGTTGGTAAAGACTCTCAGTGGCCACGAGGGAGCTGTGAAATGCCTGTTCTTTGACC AGTGGCATCTCCTCTCAGGAAGCACTGATGGCCTGGTCATGGCCTGGAGCATGGTGGGGAAGTACGAGCG CTGCCTGATGGCCTTCAAGCATCCCAAAGAGGTGCTCGACGTGTCCCTTCTCTTCCTCCGGGTCATCAGC GCCTGTGCAGATGGCAAGATCCGAATTTACAATTTCTTCAATGGGAACTGTATGAAGGTGATAAAAGCCA ATGGCAGAGGCGATCCTGTGCTGTCCTTCTTTATTCAGGGCAACAGTGATCACTTACGGAAGACCTATCT GCCAGTTGAGTGGAAGACAGATGGAAGGAGGGTTAGGAAGAAGGCAGAGAAGTCAGCTGGGATGTGCTGT AATAACCCACGCAAAACCGATGATGGGCTGAAGCTAAAGCACTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001282540
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282540.1, NP_001269469.1
RefSeq Size	3215 bp
RefSeq ORF	2145 bp
Locus ID	374286
Gene Summary	Members of the F-box protein family, such as FBXW10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603034), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) has alternate 3' exon structure and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct and shorter C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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