RNF98 (TRIM36) (NM_001300759) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ANPH; HAPRIN; RBCC728; RNF98
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300759, the custom clone sequence may differ by one or more nucleotides ATGGAGGGCGATGGCTCAGATTCGCCGGTTACCATTAAGAATATCGAAAGGGAGCTCATTTGCCCAGCAT GCAAGGAGCTGTTTACCCACCCATTGATTCTCCCTTGCCAACATAGTATCTGTCATAAATGTGTAAAAGA ACTCCTGCTGACTCTCGATGATTCATTCAACGATGTGGGATCAGACAACTCCAATCAAAGCAGTCCTCGA CTTCGGCTCCCCTCCCCTAGTATGGATAAAATTGACCGAATTAACAGACCAGGCTGGAAGCGCAATTCAT TGACCCCGAGGACAACTGTTTTCCCTTGCCCTGGCTGTGAGCATGATGTGGATCTTGGAGAACGAGGAAT CAATGGTCTGTTTCGAAACTTCACTTTGGAAACTATTGTGGAAAGATATCGTCAAGCAGCTAGGGCAGCC ACAGCCATTATGTGTGACCTTTGTAAACCACCACCTCAAGAATCCACAAAAAGCTGCATGGACTGTAGTG CAAGTTACTGCAATGAATGCTTCAAAATTCATCACCCTTGGGGTACTATAAAAGCTCAACATGAGTATGT TGGTCCAACTACTAACTTCAGACCCAAGATTTTAATGTGCCCAGAACATGAAACAGAGAGAATAAACATG TACTGTGAATTATGTAGGAGGCCAGTTTGCCATCTGTGTAAGTTGGGTGGTAATCATGCCAACCACCGTG TAACCACTATGAGCAGTGCCTACAAAACCTTAAAGGAAAAGCTTTCAAAGGATATTGATTACCTTATTGG TAAGGAAAGCCAGGTGAAGAGTCAAATATCTGAACTAAACTTGTTAATGAAAGAAACAGAGTGTAATGGA GAGAGGGCTAAAGAAGAAGCAATTACACATTTTGAAAAGCTCTTTGAAGTTCTGGAAGAGAGGAAATCAT CTGTTTTGAAAGCAATTGACTCCTCTAAGAAACTAAGATTAGACAAATTTCAGACTCAAATGGAAGAGTA CCAGGGACTTCTAGAGAACAATGGACTTGTGGGATATGCTCAAGAAGTGCTAAAGGAGACAGATCAGTCT TGCTTTGTGCAGACAGCAAAGCAGCTCCACCTCAGAATACAGAAAGCCACAGAATCTTTGAAGAGCTTTA GACCTGCAGCTCAGACTTCTTTTGAAGACTATGTTGTTAATACCTCTAAACAAACAGAACTTCTTGGAGA ATTATCCTTTTTCTCTAGTGGCATAGACGTGCCAGAGATCAATGAGGAACAGAGCAAAGTTTATAACAAT GCCTTGATAAATTGGCACCATCCAGAAAAGGATAAAGCTGATAGCTATGTTCTTGAATATCGGAAAATCA ATAGAGATGATGAAATGTCATGGAATGAGATAGAAGTGTGTGGAACAAGTAAAATAATTCAAGACTTGGA AAACAGTAGTACCTATGCTTTCAGAGTAAGAGCTTACAAGGGTTCAATCTGTAGTCCTTGCAGCAGAGAA TTGATTCTTCATACTCCTCCAGCTCCAGTTTTCAGCTTCCTCTTTGATGAAAAATGTGGCTATAATAATG AACACCTCCTGCTGAACTTGAAGAGAGACCGTGTAGAGAGTAGAGCTGGATTTAATCTTCTGCTTGCTGC AGAACGCATCCAAGTGGGTTATTACACAAGCTTAGACTACATCATTGGAGATACTGGCATTACAAAAGGA AAACACTTCTGGGCCTTCCGTGTGGAACCATATTCATACCTGGTAAAAGTGGGAGTTGCTTCTAGCGATA AACTACAAGAATGGCTCCGTTCTCCCCGGGATGCAGTTAGTCCAAGATATGAGCAAGACAGTGGGCATGA CAGTGGAAGTGAGGATGCCTGTTTTGATTCTTCACAACCATTTACCTTAGTTACTATAGGCATGCAGAAA TTTTTTATACCCAAGTCACCTACTTCTTCTAATGAACCTGAAAATAGAGTTCTCCCTATGCCAACAAGTA TTGGGATTTTCCTTGACTGTGATAAAGGCAAAGTAGATTTCTATGATATGGATCAGATGAAATGCCTTTA TGAACGCCAAGTGGACTGTTCACATACACTGTATCCAGCATTTGCATTAATGGGCAGTGGAGGAATTCAG CTTGAAGAACCCATCACAGCAAAATATCTGGAATACCAAGAGGACATGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001300759
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300759.1, NP_001287688.1
RefSeq Size	4240 bp
RefSeq ORF	2151 bp
Locus ID	55521
UniProt ID	Q9NQ86
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (5) has an alternate 5' terminal exon, compared to variant 1. The resulting isoform (5) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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