TLE 1 (TLE1) (NM_001303104) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ESG; ESG1; GRG1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001303104, the custom clone sequence may differ by one or more nucleotides ATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCTGCAGGCCAGCCCTTCAAGTTCACTATCCCGG AGTCCCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAATG TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATATGGA TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATCC CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAGGTGACCATGGC AGAATTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTTTCTCATGGCCACGGACCCCCA GTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCTGGAATCCCGCCCCTCGGGGGCAGTGCCGGCC TTCTTGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCACGA TGCAGAGCACCACAGAGACAGAGAGCCGGGCACAAGTAATTCCCTCCTGGTCCCAGACAGTCTAAGAGGC ACAGATAAACGCAGAAATGGACCTGAATTTTCCAATGACATCAAGAAAAGGAAGGTGGATGATAAGGACT CCAGCCACTATGACAGTGATGGTGACAAAAGCGATGACAACTTAGTTGTGGATGTGTCTAATGAGGACCC TTCTTCTCCGCGAGCAAGCCCTGCCCACTCGCCCCGGGAAAATGGAATCGACAAAAATCGCCTGCTAAAG AAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCGGCAAGTTCCACTTCTTTGAAATCCAAAGAAA TGAGCTTGCATGAAAAAGCCAGCACGCCTGTTCTGAAATCCAGCACACCAACGCCTCGGAGCGACATGCC AACGCCGGGCACCAGCGCCACTCCAGGCCTCCGTCCAGCAGCTGGCTTGAGGACACCCCTGGCAGTGCCC GGCCCATATCCTGCTCCTTTTGGGATGGTCCCCCACGCTGGCATGAACGGCGAGCTGACCAGCCCAGGCG CTGCCTACGCCAGTTTACACAACATGTCGCCCCAGATGAGCGCCGCAGCCGCCGCGGCCGCCGTGGTGGC CTACGGGCGCTCCCCCATGGTGGGGTTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCTCCAAAC CTGGCAGGAATCCCTGGGGGGAAACCTGCATACTCCTTCCACGTTACTGCAGACGGTCAGATGCAGCCTG TCCCTTTTCCCCCCGACGCCCTCATCGGACCCGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAA CCACGGGGAGGTGGTGTGCGCTGTGACCATCAGCAACCCCACGAGACACGTGTACACAGGCGGGAAGGGC TGCGTCAAGGTCTGGGACATCAGCCACCCTGGCAATAAGAGCCCTGTCTCCCAGCTCGACTGTCTGAACA GAGACAATTATATCCGTTCCTGTAAATTGCTACCCGATGGCTGCACTCTCATAGTGGGAGGGGAAGCCAG TACTTTGTCCATTTGGGACCTGGCGGCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCGGCCCCC GCCTGCTACGCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCG CTGTGTGGGATCTGCACAACCAGACACTAGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGCTGTAT TGACATTTCTAATGATGGCACCAAGCTCTGGACGGGTGGTTTGGACAACACAGTCAGGTCCTGGGACCTG CGCGAGGGGCGGCAGCTGCAGCAGCACGACTTCACCTCCCAGATCTTCTCCCTGGGGTACTGCCCCACCG GGGAGTGGCTGGCAGTGGGCATGGAGAGCAGCAATGTGGAGGTGCTGCACGTGAACAAGCCTGACAAGTA CCAGCTGCACCTGCATGAGAGCTGCGTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTTGTGAGT ACTGGAAAAGATAACCTCCTCAATGCTTGGCGGACCCCCTATGGAGCCAGCATATTCCAGTCCAAAGAGT CCTCGTCAGTGCTTAGCTGTGACATCTCTGTGGATGATAAGTACATAGTCACTGGCTCGGGGGACAAGAA GGCTACAGTCTATGAAGTCATCTACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001303104
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303104.1, NP_001290033.1
RefSeq Size	4092 bp
RefSeq ORF	2268 bp
Locus ID	7088
UniProt ID	Q04724
Protein Families	Transcription Factors
Gene Summary	Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses two alternate in-frame splice sites compared to variant 1. The encoded isoform (3) is shorter than isoform 1.

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