Gamma tubulin complex component 3 (TUBGCP3) (NM_001286278) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	104p; ALP6; GCP3; Grip104; SPBC98; Spc98; Spc98p
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286278, the custom clone sequence may differ by one or more nucleotides ATGGCGACCCCGGACCAGAAGTCGCCGAACGTTCTGCTGCAGAACCTGTGCTGCAGGATCCTGGGCAGGA GCGAAGCTGATGTAGCCCAGCAGTTCCAGTATGCTGTGCGGGTGATTGGCAGCAACTTCGCCCCAACTGT TGAAAGAGATGAATTTTTAGTAGCTGAAAAAATCAAGAAAGAGCTTATTCGACAACGAAGAGAAGCAGAT GCTGCATTATTTTCAGAACTCCACAGAAAACTTCATTCACAGGGAGTTTTGAAAAATAAATGGTCAATAC TCTACCTCTTGCTGAGCCTCAGTGAGGACCCACGCAGGCAGCCAAGCAAGGTTTCTAGCTATGCTACGTT ATTTGCTCAGGCCTTACCAAGAGATGCCCACTCAACCCCTTACTACTATGCCAGGCCTCAGACCCTTCCC CTGAGCTACCAAGATCGGAGTGCCCAGTCAGCCCAGAGCTCCGGCAGCGTGGGCAGCAGTGGCATCAGCA GCATTGGCCTGTGTGCCCTCAGTGGCCCCGCGCCTGCGCCACAATCTCTCCTCCCAGGACAGTCTAATCA AGCTCCAGGAGTAGGAGATTGCCTTCGACAGCAGTTGGGGTCACGACTCGCATGGACTTTAACTGCAAAT CAGCCTTCTTCACAAGCCACTACCTCAAAAGGTGTCCCCAGTGCTGTGTCTCGCAACATGACAAGGTCCA GGAGAGAAGGGGATACGGGTGGTACTATGGAAATTACAGAAGCAGCTCTGGTAAGGGACATTTTGTACGT CTTTCAGGGCATAGATGGCAAAAACATCAAAATGAACAACACTGAAAATTGTTACAAAGTAGAAGGAAAG GCAAATCTAAGTAGGTCTTTGAGAGACACAGCAGTCAGGCTTTCTGAGTTGGGATGGTTGCATAATAAAA TCAGAAGATACACGGACCAGAGGAGCCTGGACCGCTCATTCGGACTCGTCGGGCAGAGCTTTTGTGCTGC CTTGCACCAGGAACTCAGAGAATACTATCGATTGCTCTCTGTTTTACATTCTCAGCTACAACTAGAGGAT GACCAGGGTGTGAATTTGGGACTTGAGAGTAGTTTAACACTTCGGCGCCTCCTGGTTTGGACCTATGATC CCAAAATACGACTGAAGACCCTTGCGGCCCTAGTGGACCACTGCCAAGGAAGGAAAGGAGGTGAGCTGGC CTCAGCTGTCCACGCCTACACAAAAACAGGAGACCCGTACATGCGGTCTCTGGTGCAGCACATCCTCAGC CTCGTGTCTCATCCTGTTTTGAGCTTCCTGTACCGCTGGATATATGATGGGGAGCTTGAGGACACTTACC ACGAATTTTTTGTAGCATCAGATCCAACAGTTAAAACAGATCGACTGTGGCACGACAAGTATACTTTGAG GAAATCGATGATTCCTTCGTTTATGACGATGGATCAGTCTAGGAAGGTCCTTTTGATAGGAAAATCAATA AATTTCTTGCACCAAGTTTGTCATGATCAGACTCCCACTACAAAGATGATAGCTGTGACCAAGTCTGCAG AGTCACCCCAGGACGCTGCAGACCTATTCACAGACTTGGAAAATGCATTTCAGGGGAAGATTGATGCTGC TTATTTTGAGACCAGCAAATACCTGTTGGATGTTCTCAATAAAAAGTACAGCTTGCTGGACCACATGCAG GCAATGAGGCGGTACCTGCTTCTTGGTCAAGGAGACTTTATAAGGCACTTAATGGACTTGCTAAAACCAG AACTTGTCCGTCCAGCTACGACTTTGTATCAGCATAACTTGACTGGAATTCTAGAAACCGCTGTCAGAGC CACCAACGCACAGTTTGACAGTCCTGAGATCCTGCGAAGGCTGGACGTGCGGCTGCTGGAGGTCTCTCCA GGTGACACTGGATGGGATGTCTTCAGCCTCGATTATCATGTTGACGGACCAATTGCAACTGTGTTTACTC GAGAATGTATGAGCCACTACCTAAGAGTATTTAACTTCCTCTGGAGGGCGAAGCGGATGGAATACATCCT CACTGACATACGGAAGGGACACATGTGCAATGCAAAGCTCCTGAGAAACATGCCAGAGTTCTCCGGGGTG CTGCACCAGTGTCACATTTTGGCCTCTGAGATGGTCCATTTCATTCATCAGATGCAGTATTACATCACAT TTGAGGTGCTTGAATGTTCTTGGGATGAGCTTTGGAACAAAGTCCAGCAGGCCCAGGATTTGGATCACAT CATTGCTGCACACGAGGTGTTCTTAGACACCATCATCTCCCGCTGCCTGCTGGACAGTGACTCCAGGGCA CTTTTAAATCAACTTAGAGCTGTGTTTGATCAAATTATTGAACTTCAGAATGCTCAAGATGCAATATACA GAGCTGCTCTGGAAGAATTGCAGAGACGATTACAGTTTGAAGAGAAAAAGAAACAGCGTGAAATTGAGGT TGAGATGTGCCTATACTGCGTTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286278
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286278.1, NP_001273207.1
RefSeq Size	2796 bp
RefSeq ORF	2475 bp
Locus ID	10426
UniProt ID	Q96CW5
Protein Families	Druggable Genome
Gene Summary	Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks multiple 3' exons and contains an alternate 3' terminal exon, resulting in a distinct 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1.

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