BRD8 (NM_001300962) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	p120; SMAP; SMAP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300962, the custom clone sequence may differ by one or more nucleotides ATGGTCCATCCGAGAGAAGCTATGTTTAGCATCTTCTGTCATGAGAAGTGGCGATCAAAATTGCATTGTG CTTCCCAGTACTCGGAGCTTTTAGAGACCACTGAGACACCAAAACGGAAACGAGGTGAAAAGGGAGAAGT GGTGGAAACTGTTGAAGATGTTATTGTTCGGAAATTGACTGCTGAGCGAGTTGAAGAACTAAAGAAAGTG ATAAAGGAAACCCAGGAGAGATATAGACGGCTAAAGAGAGATGCAGAACTAATTCAAGCTGGACACATGG ACAGCAGACTGGATGAGCTTTGCAATGACATTGCAACGAAAAAGAAATTGGAAGAAGAGGAGGCTGAAGT AAAGAGGAAGGCTACAGATGCTGCATACCAGGCTCGTCAAGCAGTAAAAACACCCCCCCGGAGGTTACCC ACTGTGATGGTTCGCTCTCCTATAGATTCTGCCTCCCCAGGAGGTGATTATCCACTTGGGGACTTGACTC CAACCACTATGGAAGAGGCTACCTCTGGGGTCAATGAGAGTGAAATGGCTGTGGCTTCTGGCCACCTGAA CAGTACAGGTGTCCTCCTGGAGGTAGGCGGGGTCCTTCCCATGATACATGGTGGGGAGATACAGCAAACA CCCAATACTGTTGCAGCCTCCCCTGCTGCATCAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGCTGGTC CTACACAGTTCACCACACCTCTTGCTTCCTTCACTACTGTTGCCAGTGAGCCTCCAGTTAAACTTGTGCC ACCCCCTGTAGAGTCTGTGTCCCAAGCTACCATTGTCATGATGCCTGCGCTGCCAGCACCATCCTCTGCT CCGGCTGTCTCCACTACTGAAAGTGTAGCTCCAGTGAGTCAACCCGACAACTGTGTTCCCATGGAGGCTG TGGGGGATCCACATACTGTGACTGTTTCCATGGACAGCAGTGAAATATCCATGATCATCAATTCTATCAA AGAAGAGTGTTTTCGATCAGGGGTAGCAGAGGCTCCTGTTGGATCAAAGGCTCCCAGCATAGATGGGAAG GAAGAATTAGATCTGGCTGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGCTGGATTTTGAGA CTGTTGGAGACATCATTGCCATCATTGAGGACAAGGTAGATGATCATCCTGAAGTGCTGGATGTGGCAGC AGTGGAAGCAGCACTGTCATTTTGTGAAGAAAATGATGATCCTCAGTCCCTGCCTGGCCCCTGGGAGCAT CCTATCCAGCAGGAGCGGGACAAGCCAGTACCTCTCCCTGCACCAGAAATGACGGTCAAGCAAGAGAGAC TGGACTTTGAGGAAACGGAAAACAAGGGAATACATGAACTGGTGGACATCAGGGAGCCCAGTGCAGAGAT CAAGGTGGAACCTGCAGAACCAGAGCCAGTCATTTCAGGAGCCGAAATAGTAGCTGGAGTTGTTCCAGCC ACAAGTATGGAGCCACCAGAACTCAGGAGTCAGGACTTAGATGAGGAACTGGGAAGTACTGCAGCTGGAG AGATTGTTGAAGCAGATGTTGCCATTGGGAAAGGCGATGAGACTCCACTTACAAATGTGAAGACAGAGGC ATCCCCTGAAAGCATGTTGTCTCCATCACATGGCTCAAATCCCATTGAAGATCCTTTAGAGGCAGAGACT CAGCACAAGTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGCCAGATAAAGG ATGCCCCAGGTGAGGATGAGGAGGAAGATGGTGTCAGTGAAGCGGCCAGCCTAGAGGAGCCTAAGGAAGA GGATCAAGGAGAAGGCTACTTGTCAGAAATGGATAATGAACCTCCTGTGAGCGAGAGTGATGATGGCTTC AGCATACACAATGCTACACTGCAGTCACACACACTGGCAGACTCCATCCCCAGCAGCCCTGCTTCTTCAC AGTTCTCTGTCTGTAGTGAGGATCAGGAAGCTATTCAGGCACAGAAAATTTGGAAGAAAGCCATCATGCT TGTATGGAGAGCTGCAGCTAATCATAGGTATGCCAATGTCTTCCTGCAGCCTGTTACAGATGACATAGCA CCTGGCTACCACAGCATTGTGCAGAGGCCTATGGATTTGTCAACTATTAAGAAAAACATAGAAAATGGAC TGATCCGAAGCACAGCTGAATTTCAGCGTGACATTATGCTGATGTTTCAGAATGCTGTAATGTACAATAG CTCAGACCATGATGTCTATCACATGGCAGTGGAGATGCAGCGAGATGTCTTGGAACAGATCCAGCAATTC TTGGCCACGCAGTTGATTATGCAAACATCCGAGTCTGGGATCAGTGCTAAAAGTCTTCGAGGGAGAGATT CTACCCGCAAACAGGATGCTTCAGAGAAGGACAGTGTCCCAATGGGCTCTCCTGCCTTCCTTCTCTCTCT CTTTGATGGAGGAACCAGGGGACGCCGCTGTGCCATTGAAGCAGATATGAAGATGAAAAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300962
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300962.1, NP_001287891.1
RefSeq Size	2964 bp
RefSeq ORF	2514 bp
Locus ID	10902
UniProt ID	Q9H0E9
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (6) lacks two internal exons in the 5' coding region, compared to variant 1. The resulting isoform (6) has a distinct N-terminus and lacks an internal segment, compared to isoform 1.

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