DNAH2 (NM_001303270) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DNAHC2; DNHD3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001303270, the custom clone sequence may differ by one or more nucleotides ATGTCCAGCAAAGCTGAGAAGAAGCAGCGATTGAGTGGCCGAGGAAGCTCCCAGGCAAGCTGGTCAGGGC GGGCCACTCGGGCTGCTGTGGCCACACAGGAGCAGGGGAATGCCCCGGCTGTCAGTGAGCCAGAGCTGCA GGCTGAGCTCCCCAAGGAGGAGCCTGAGCCACGGTTGGAGGGACCTCAAGCACAGAGTGAAGAATCAGTG GAGCCCGAGGCAGATGTGAAGCCCCTCTTCCTTTCCCGAGCTGCGCTGACAGGACTGGCGGATGCAGTGT GGACACAGGAGCATGATGCCATTCTGGAACACTTTGCCCAGGACCCTACAGAATCCATCCTCACCATCTT CATTGACCCTTGTTTTGGGCTGAAGCTAGAGCTGGGCATGCCTGTACAGACCCAGAACCAGCTTGTCTAC TTCATTCGCCAAGCACCAGTTCCCATCACCTGGGAGAACTTCGAGGCAACTGTGCAGTTTGGGACGGTGC GGGGCCCCTATATCCCGGCCCTGCTTCGGCTGCTCGGTGGAGTCTTTGCCCCTCAGATCTTTGCAAACAC AGGCTGGCCTGAGAGCATTAGAAATCATTTTGCTTCTCATCTGCACAAGTTCTTGGCCTGCCTGACAGAC ACTCGGTACAAACTGGAGGGGCACACGGTCCTCTACATCCCTGCAGAGGCCATGAACATGAAGCCTGAGA TGGTGATAAAGGACAAAGAGCTGGTGCAACGGCTAGAGACCTCCATGATCCACTGGACCCGGCAGATAAA GGAGATGCTCAGTGCCCAGGAGACTGTGGAGACAGGAGAAAATTTAGGTCCTCTGGAGGAGATTGAGTTC TGGCGCAACCGATGCATGGACCTGTCTGGCATCAGTAAGCAGCTGGTGAAGAAGGGAGTGAAGCACGTTG AATCCATCCTGCACCTTGCCAAGTCGTCCTACTTGGCGCCCTTTATGAAACTGGCACAGCAGATCCAGGA TGGCTCTCGTCAAGCACAGTCAAACCTGACCTTTTTGTCAATCCTGAAGGAACCTTACCAGGAGTTGGCT TTCATGAAGCCCAAGGACATCTCTAGCAAGCTCCCTAAGCTGATCAGTCTCATCCGCATCATCTGGGTCA ACTCTCCCCACTACAACACTCGGGAGAGACTGACCTCGCTCTTCCGAAAGATGAGCAATGAGATCATCCG CTTATGCTGCCACGCCATCTCCCTGGACCGGATCTTTGAGGGATATGTCTCTTCCAGCAAGGAGGACCTG CAAGGCTGCATTCTCTGTTGTCACGCTTGGAAAGATCACTACGTACAGGCTGTGCAGATGCACATCCAGT TCTCCAGTCGGGGCTGGGTCCTAGATCAGACCAGCATCTTTGCTCAGGTTGATGCCTTTGTGCAGCGCTG CAAGGACCTTATTGAGGTATGTGACTGTCAGTATCACTTCGCCCGCTGGGAAGATGGCAAGCAGGGTCCC CTTCCTTGCTTCTTTGGTGCCCAGGGGCCACAGATAACACGGAACTTGCTGGAGATTGAGGACATCTTTC ATAAAAATCTGCACACGCTGCGAGCCGTTCGCGGGGGTATCCTGGATGTCAAGAACACCTGTTGGCATGA AGACTACAATAAGTTCCGTGCCGGAATCAAGGACCTGGAGGTGATGACCCAGAACCTGATCACCTCAGCC TTCGAGTTGGTGCGGGACGTGCCGCACGGCGTGCTTCTGCTGGACACCTTCCACAGGCTTGCCTCCCGCG AGGCTATCAAGCGGACTTATGACAAGAAGGCGGTGGATCTCTACATGCTGTTCAATAGCGAGCTGGCCCT GGTGAACCGTGAACGGAACAAGAAATGGCCAGACCTGGAGCCCTACGTGGCCCAGTATTCCGGAAAGGCG CGCTGGGTGCACATCCTCCGGCGTCGCATCGACAGAGTCATGACCTGCCTTGCTGGTGCTCATTTCCTGC CCCGTATTGGGACTGGAAAGGAGAGTGTGCACACCTATCAGCAGATGGTCCAGGCCATTGATGAGCTGGT TCGAAAAACCTTCCAAGAGTGGACATCAAGTCTGGACAAGGATTGCATTCGGCGGTTGGATACCCCATTG CTGCGAATCAGCCAGGAGAAGGCGGGCATGCTGGATGTCAACTTTGACAAGTACAGGAGCCACCTGGCCC CTTTTCCCTATACTCCCCTTCTGCAGCTCTCCCAAGAATTTCACTCCCATCTTTTGACTCCTCTCTTCAT TATTCTCTCTCTTTCTCATACAATATGTTTGCTATCGTCATTTTACTTTTTTTTTTCTTCTTTTATTTTT GTCTCTCCCCACCTCCCACCCTGCTATCAACATTTTAATTTTACAACTTATCTTAAAACACAACAAAACA AAACAATGATCGGCCAGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGC ATCGCTTGAGCCCAGGAGTTTGAGAACAGCCTGGGCAACATGGCAAAACCCAGTCTCTGCAAAAAATACA AAGATTAGCTGGGCGTGGTGGCACAAGCCTGTAGTCTCAGCTACTTGGGAGGGTGAGGTGGGAGGATCGC CTGAGCCCGGGAGGCAGAGGCTGCAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001303270
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303270.1, NP_001290199.1
RefSeq Size	3006 bp
RefSeq ORF	2619 bp
Locus ID	146754
UniProt ID	Q9P225
Protein Pathways	Huntington's disease
Gene Summary	Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains. The axonemal dyneins, found in cilia and flagella, are components of the outer and inner dynein arms attached to the peripheral microtubule doublets. DNAH2 is an axonemal inner arm dynein heavy chain (Chapelin et al., 1997 [PubMed 9256245]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) contains multiple differences in the UTRs and coding region, compared to variant 1. It lacks multiple 3' coding exons and contains an alternate 3' coding region. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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