eIF3s8 (EIF3C) (NM_001286478) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	eIF3-p110; EIF3CL; EIF3S8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286478, the custom clone sequence may differ by one or more nucleotides ATGTCGCGGTTTTTCACCACCGGTTCGGACAGCGAGTCCGAGTCGTCCTTGTCCGGGGAGGAGCTCGTCA CCAAACCTGTCGGAGGCAACTATGGCAAACAGCCATTGTTGCTGAGCGAGGATGAAGAAGATACCAAGAG AGTTGTCCGCAGTGCCAAGGACAAGAGGTTTGAGGAGCTGACCAACCTTATCCGGACCATCCGTAATGCC ATGAAGATTCGTGATGTCACCAAGTGCCTGGAAGAGTTTGAGCTCCTGGGAAAAGCATATGGGAAGGCCA AAAGCATTGTGGACAAAGAAGGTGTCCCCCGGTTCTATATCCGCATCCTGGCTGACCTAGAGGACTATCT TAATGAGATGAACAAGAACAATGCCAAGGCTCTGAGCACCTTGCGTCAGAAGATCCGAAAATACAACCGT GATTTCGAGTCCCATATCACAAGCTACAAGCAGAACCCCGAGCAGTCTGCGGATGAAGATGCTGAGAAAA ATGAGGAGGATTCAGAAGGCTCTTCAGATGAGGATGAGGATGAGGACGGAGTCAGTGCTGCAACTTTCTT GAAGAAGAAATCAGAAGCTCCTTCTGGGGAGAGTCGCAAGTTCCTCAAAAAGATGGATGATGAAGATGAG GACTCAGAAGATTCCGAAGATGATGAAGACTGGGACACAGGTTCCACATCTTCCGATTCCGACTCAGAGG AGGAAGAAGGGAAACAAACCGCGCTGGCCTCAAGATTTCTTAAAAAGGCACCCACCACAGATGAGGACAA GAAGGCAGCCGAGAAGAAACGGGAGGACAAAGCTAAGAAGAAGCACGACAGGAAATCCAAGCGCCTGGAT GAGGAGGAGGAGGACAATGAAGGCGGGGAGTGGGAAAGGGTCCGGGGCGGAGTGCCGTTGGTTAAGGAGA AGCCAAAAATGTTTGCCAAGGGAACTGAGATCACCCATGCTGTTGTTATCAAGAAACTGAATGAGATCCT ACAGGCACGAGGCAAGAAGGGAACTGATCGTGCTGCCCAGATTGAGCTGCTGCAACTGCTGGTTCAGATT GCAGCGGAAAACAACCTGGGAGAGGGCGTCATTGTCAAGATCAAGTTCAATATCATCGCCTCTCTCTATG ACTACAACCCCAACCTGGCAACCTACATGAAGCCAGAGATGTGGGGGAAGTGCCTGGACTGCATCAATGA GCTGATGGATATCCTGTTTGCAAATCCCAACATTTTTGTTGGAGAGAATATTCTGGAAGAGAGTGAGAAC CTGCACAACGCTGACCAGCCACTGCGTGTCCGTGGCTGCATCCTAACTCTGGTGGAACGAATGGATGAAG AATTTACCAAAATAATGCAAAATACTGACCCTCACTCCCAAGAGTACGTGGAGCACTTGAAGGATGAGGC CCAGGTGTGTGCCATCATCGAGCGTGTGCAGCGCTACCTGGAGGAGAAGGGCACTACCGAGGAGGTCTGC CGCATCTACCTGCTGCGCATCCTGCACACCTACTACAAGTTTGATTACAAGGCCCATCAGCGACAGCTGA CCCCGCCTGAGGGCTCCTCAAAGTCTGAGCAAGACCAGGCAGAAAATGAGGGCGAGGACTCGGCTGTGTT GATGGAGAGACTGTGCAAGTACATCTACGCCAAGGACCGCACAGACCGGATCCGCACATGTGCCATCCTC TGCCACATCTACCACCATGCTCTGCACTCGCGCTGGTACCAGGCCCGCGACCTCATGCTCATGAGCCACT TGCAGGACAACATTCAGCATGCAGACCCGCCAGTGCAGATCCTTTACAACCGCACCATGGTGCAGCTGGG CATCTGTGCCTTCCGCCAAGGCCTGACCAAGGACGCACACAACGCCCTGCTGGACATCCAGTCGAGTGGC CGAGCCAAGGAGCTTCTGGGCCAGGGCCTGCTGCTGCGCAGCCTGCAGGAGCGCAACCAGGAGCAGGAGA AGGTGGAGCGGCGCCGTCAGGTCCCCTTCCACCTGCACATCAACCTGGAGCTGCTGGAGTGTGTCTACCT GGTGTCTGCCATGCTCCTGGAGATCCCCTACATGGCCGCCCATGAGAGCGATGCCCGCCGACGCATGATC AGCAAGCAGTTCCACCACCAGCTGCGCGTGGGCGAGCGACAGCCCCTGCTGGGTCCCCCTGAGTCCATGC GGGAACATGTGGTCGCTGCCTCCAAGGCCATGAAGATGGGTGACTGGAAGACCTGTCACAGTTTTATCAT CAATGAGAAGATGAATGGGAAAGTGTGGGACCTTTTCCCCGAGGCTGACAAAGTCCGCACCATGCTGGTT AGGAAGATCCAGGAAGAGTCACTGAGGACCTACCTCTTCACCTACAGCAGTGTCTATGACTCCATCAGCA TGGAGACGCTGTCAGACATGTTTGAGCTGGATCTGCCCACTGTGCACTCCATCATCAGCAAAATGATCAT TAATGAGGAGCTGATGGCCTCCCTGGACCAGCCAACACAGACAGTGGTGATGCACCGCACTGAGCCCACT GCCCAGCAGAACCTGGCTCTGCAGCTGGCCGAGAAGCTGGGCAGCCTGGTGGAGAACAACGAACGGGTGT TTGACCACAAGCAGGGCACCTACGGGGGCTACTTCCGAGACCAGAAGGACGGCTACCGCAAAAACGAGGG CTACATGCGCCGCGGTGGCTACCGCCAGCAGCAGTCTCAGACGGCCTACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286478
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286478.1, NP_001273407.1
RefSeq Size	3066 bp
RefSeq ORF	2712 bp
Locus ID	8663
UniProt ID	Q99613
Protein Families	Druggable Genome
Gene Summary	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

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