USP38 (NM_001290325) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HP43.8KD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290325, the custom clone sequence may differ by one or more nucleotides ATGGACAAGATCCTGGAGGGCCTTGTGAGTTCCTCGCATCCCCTGCCCCTCAAGCGGGTGATTGTGCGGA AGGTGGTGGAATCGGCGGAGCACTGGCTAGACGAGGCGCAGTGCGAGGCCATGTTTGACCTGACGACCCG GCTCATCCTGGAGGGCCAGGACCCTTTCCAGCGGCAGGTGGGGCACCAGGTGCTGGAGGCCTACGCACGA TACCACCGGCCAGAGTTCGAGTCCTTCTTCAACAAGACCTTCGTGTTGGGCCTCCTTCATCAGGGCTACC ACTCTCTGGACAGGAAGGATGTAGCCATCCTGGACTACATTCACAACGGCCTGAAGCTGATTATGAGCTG TCCGTCGGTGCTGGATCTCTTTAGCCTCCTGCAGGTAGAGGTGTTACGGATGGTGTGTGAGAGGCCGGAG CCGCAGCTCTGTGCCCGACTGAGCGACCTTCTGACCGACTTTGTGCAATGCATCCCCAAGGGGAAATTGT CCATCACGTTCTGTCAACAGCTGGTTCGAACGATAGGCCATTTCCAGTGCGTGTCCACCCAGGAAAGAGA GCTGCGGGAATATGTCTCCCAGGTGACAAAAGTGAGTAACTTGCTGCAGAACATCTGGAAGGCCGAGCCT GCCACACTACTGCCTTCCCTGCAAGAAGTTTTTGCAAGCATCTCTTCCACAGATGCATCATTTGAACCTT CTGTAGCATTGGCAAGCCTTGTGCAGCATATTCCTCTTCAGATGATTACAGTTCTCATCAGGAGCCTTAC TACGGATCCAAATGTAAAAGATGCAAGTATGACCCAAGCCCTTTGCAGAATGATTGACTGGCTATCCTGG CCATTGGCTCAGCATGTGGATACATGGGTAATTGCACTCCTGAAAGGACTGGCAGCTGTCCAGAAGTTTA CTATTTTGATAGATGTTACTTTGCTGAAAATAGAACTGGTTTTTAATCGACTTTGGTTTCCTCTTGTGAG ACCTGGTGCTCTTGCAGTTCTTTCTCACATGCTGCTTAGCTTTCAGCATTCTCCAGAGGCGTTCCATTTG ATTGTTCCTCATGTGGTTAATTTGGTTCATTCTTTCAAAAATGATGGTCTGCCTTCAAGTACAGCCTTCT TAGTACAATTAACAGAATTGATACACTGTATGATGTATCATTATTCTGGATTTCCAGATCTCTATGAACC TATTCTGGAGGCAATAAAGGATTTTCCTAAGCCCAGTGAAGAGAAGATTAAGTTAATTCTCAATCAAAGT GCCTGGACTTCTCAATCCAATTCTTTGGCGTCTTGCTTGTCTAGACTTTCTGGAAAATCTGAAACTGGGA AAACTGGTCTTATTAACCTAGGAAATACATGTTATATGAACAGTGTTATACAAGCCTTGTTTATGGCCAC AGATTTCAGGAGACAAGTATTATCTTTAAATCTAAATGGGTGCAATTCATTAATGAAAAAATTACAGCAT CTTTTTGCCTTTCTGGCCCATACACAGAGGGAAGCATACGCACCTCGGATATTCTTTGAGGCTTCCAGAC CTCCATGGTTTACTCCCAGATCACAGCAAGACTGTTCTGAATACCTCAGATTTCTCCTTGACAGGCTCCA TGAAGAAGAAAAGATCTTGAAAGTTCAGGCCTCACACAAGCCTTCTGAAATTCTGGAATGCAGTGAAACT TCTTTACAGGAAGTAGCTAGTAAAGCAGCAGTACTAACAGAGACCCCTCGTACAAGTGACGGTGAGAAGA CTTTAATAGAAAAAATGTTTGGAGGAAAACTACGAACTCACATACGTTGTTTGAACTGCAGGAGTACCTC ACAAAAAGTGGAAGCCTTTACAGATCTTTCGCTTGCCTTTTGTCCTTCCTCTTCTTTGGAAAACATGTCT GTCCAAGATCCAGCATCATCACCCAGTATACAAGATGGTGGTCTAATGCAAGCCTCTGTACCCGGTCCTT CAGAAGAACCAGTAGTTTATAATCCAACAACAGCTGCCTTCATCTGTGACTCACTTGTGAATGAAAAAAC CATAGGCAGTCCTCCTAATGAGTTTTACTGTTCTGAAAACACTTCTGTCCCTAACGAATCTAACAAGATT CTTGTTAATAAAGATGTACCTCAGAAACCAGGAGGTGAAACCACACCTTCAGTAACTGACTTACTAAATT ATTTTTTGGCTCCAGAGATTCTTACTGGTGATAACCAATATTATTGTGAAAACTGTGCCTCTCTGCAAAA TGCTGAGAAAACTATGCAAATCACGGAGGAACCTGAATACCTTATTCTTACTCTCCTGAGATTTTCATAT GATCAGAAGTATCATGTGAGAAGGAAAATTTTAGACAATGTATCACTGCCACTGGTTTTGGAGTTGCCAG TTAAAAGAATTACTTCTTTCTCTTCATTGTCAGAAAGTTGGTCTGTAGATGTTGACTTCACTGATCTTAG TGAGAACCTTGCTAAAAAATTAAAGCCTTCAGGGACTGATGAAGCTTCCTGCACAAAATTGGTGCCCTAT CTATTAAGTTCCGTTGTGGTTCACTCTGGTATATCCTCTGAAAGTGGGCATTACTATTCTTATGCCAGGA ATATCACAAGTACAGACTCTTCATATCAGATGTACCACCAGTCTGAGGCTCTGGCATTAGCATCCTCCCA GAGTCATTTACTAGGGAGAGATAGTCCCAGTGCAGTTTTTGAACAGGATTTGGAAAATAAGGAAATGTCA AAAGAATGGTTTTTATTTAATGACAGTAGAGTGACATTTACTTCATTTCAGTCAGTCCAGAAAATTACGA GCAGGTTTCCAAAGGACACAGCTTATGTGCTTTTGTATAAAAAACAGCATAGTACTAATGGTTTAAGTGG TAATAACCCAACCAGTGGACTCTGGATAAATGGAGACCCACCTCTACAGAAAGAACTTATGGATGCTATA ACAAAAGACAATAAACTATATTTACAGGTAAGTTGGAAATACAAGCTTTATTTGTTGAAAATATTAAACA ATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001290325
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290325.1, NP_001277254.1
RefSeq Size	4123 bp
RefSeq ORF	3015 bp
Locus ID	84640
UniProt ID	Q8NB14
Protein Families	Druggable Genome, Protease
Gene Summary	Deubiquitinating enzyme exhibiting a preference towards 'Lys-63'-linked ubiquitin chains.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains a 3' terminal exon that extends past a splice site used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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