NBPF9 (NM_001277444) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AE01
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001277444, the custom clone sequence may differ by one or more nucleotides ATGGTGGTATCAGCCGGCCCTTGGTCCAGCGAGAAGGCAGAGATGAACATTCTAGAAATCAACGAGAAAT TGCGCCCCCAGTTGGCAGAGAACAAACAGCAGTTCGGAAACCTCAAAGAGAGATGTTTTCTAACTCAACT GGCCGGCTTCCTGGCCAACCGACAGAAGAAATACAAGTATGAAGAGTGTAAAGACCTCATAAAATTTATG CTGAGGAATGAGCGACAGTTCAAGGAGGAGAAGCTTGCAGAGCAGCTGAAGCAAGCTGAGGAGCTCAGGC AATATAAAGTCCTGGTTCACTCTCAGGAACGAGAGCTGACGCAGTTAAAGGAGAAGTTACGGGAAGGGAG AGATGCCTCCCGCTCATTGAATGAGCATCTCCAGGCCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAG GGGCAGGACCTCCAAGAACAGCTGGCTGAGGGGTGTAGACTGGCACAGCACCTTGTCCAAAAGCTCAGCC CAGAAAATGACGAAGATGAGGATGAAGATGTTCAAGTTGAGGAGGATGAGAAAGTACTGGAATCATCTGC CCCCAGGGAGGTGCAGAAGGCTGAAGAGAGCAAAGTCGCTGAGGACTCACTGGAGGAATGTGCCATCACT TGTTCAAATAGCCACGGCCCTTGTGACTCCAACCAGCCTCACAAGAACATCAAAATCACATTTGAGGAAG ACGAAGTCAACTCAACTCTGGTTGTAGACAGAGAATCCTCTCATGATGAATGTCAGGATGCTCTAAACAT TCTCCCAGTCCCTGGCCCCACCTCTTCTGCCACAAACGTCAGCATGGTGGTATCAGCCGGCCCTTTGTCC AGCGAGAAGGCAGAGATGAACATTCTAGAAATCAATGAGAAATTGCGCCCCCAGCTGGCAGAGAAGAAAC AGCAGTTCAGAAACCTCAAAGAGAAATGTTTTCTAACTCAACTGGCCGGCTTCCTGGCCAACCAGCAGAA CAAATACAAGTATGAAGAGTGTAAAGACCTCATAAAATTTATGCTGAGGAATGAGCGACAGTTCAAGGAG GAGAAGCTTGCAGAGCAGCTGAAGCAAGCTGAGGAGCTCAGGCAATATAAAGTCCTGGTTCACGCTCAGG AACGAGAGCTGACCCAGTTAAGGGAGAAGTTGCGGGAAGGGAGAGATGCCTCCCGCTCATTGAATGAGCA TCTCCAGGCCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAAGAACAGCTGGCT GAGGGGTGTAGACTGGCACAGCACCTTGTCCAAAAGCTCAGCCCAGAAAATGACAACGATGACGATGAAG ATGTTCAAATTGAGGTGGCTGAGAAAGTGCAGAAATCGTCTGCCCCCAGGGAGATGCAGAAGGCTGAAGA AAAGGAAGTCCCTGAGGACTCACTGGAGGAATGTGCCATCACTTATTCAAATAGCCATGGCCCTTATGAC TCCAACCAGCCACATAGGAAAACCAAAATCACATTTGAGGAAGACAAAGTCGACTCAACTCTCATTGGCT CATCCTCTCATGTTGAACGGGAAGATGCTGTACACATTATTCCAGAAAATGAAAGTGATGATGAGGAAGA GGAAGAAAAAGGGCCAGTGTCTCCCAGGAATCTGCAGGAGTCTGAAGAGGAGGAAGTCCCCCAGGAGTCC TGGGATGAAGGTTATTCGACTCCCTCAATTCCTCCTGAAATGTTGGCCTCGTACAAGTCTTACAGCAGCA CATTTCACTCATTAGAGGAACAGCAAGTCTGCATGGCTGTTGACATAGGCAGACATCGGTGGGATCAAGT GAAAAAGGAGGACCAAGAGGCAACAGGTCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAA GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCC AGCCCTACAGAAGTGCCTTTTACGTATTGGAGCAACAGCGTGTTGGCTTGGCTGTTGACATGGATGAAAT TGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAT GAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGGTAGATGGTATTCGACTCCTTCAGGTTATCTTGAAC TGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCTCT TGACTTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGC AGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT CCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAAAACA TGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGA TCAAAGAAGAAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAAATCAAAACCCACCATGCCCCAGGCTCA GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCC TTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAG TACCTTGGCTTGGCTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCAC CATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAG ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT GCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGA AAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCC ACCATGCCCCAGGCTCAACGGCGTGCTGATGGAAGTGGAAGAGCCTGAAGTCTTGCAGGACTCACTGGAT GGATGTTATTCTACTCCGTCAATGTACTTTGAACTACCTGACTCATTCCAGCACTACAGAAGTGTGTTTT ACTCATTTGAGGAACAGCACATCAGCTTCGCCCTTTACGTGGACAATAGGTTTTTTACTTTGACGGTGAC AAGTCTCCACCTGGTGTTCCAGATGGAAGTCATATTCCCACAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277444
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277444.1, NP_001264373.1
RefSeq Size	5034 bp
RefSeq ORF	3336 bp
Locus ID	400818
UniProt ID	P0DPF3
Gene Summary	This gene is a member of the neuroblastoma breakpoint family (NBPF) which consists of dozens of recently duplicated genes primarily located in segmental duplications on human chromosome 1. This gene family has experienced its greatest expansion within the human lineage and has expanded, to a lesser extent, among primates in general. Members of this gene family are characterized by tandemly repeated copies of DUF1220 protein domains. Gene copy number variations in the human chromosomal region 1q21.1, where most DUF1220 domains are located, have been implicated in a number of developmental and neurogenetic diseases such as microcephaly, macrocephaly, autism, schizophrenia, cognitive disability, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract anomalies. Altered expression of some gene family members is associated with several types of cancer. This gene family contains numerous pseudogenes. [provided by RefSeq, Apr 2013] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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