DIS (CCAR1) (NM_001282959) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282959, the custom clone sequence may differ by one or more nucleotides ATGGCTCAATTTGGAGGACAGAAGAATCCGCCATGGGCTACTCAGTTTACAGCCACTGCAGTATCACAGC CAGCTGCACTGGGTGTTCAACAGCCATCACTCCTTGGAGCATCTCCTACCATTTATACACAGCAAACTGC ATTGGCAGCAGCAGGCCTTACCACACAAACTCCAGCAAACTATCAGTTAACACAAACTGCTGCATTGCAG CAACAAGCCGCAGCTGCAGCAGCTGCATTACAACAGTTACAGCAACCCCAGCAAACCCTCTTAACACAGC CAGCTGTTGCACTGCCTACAAGCCTTAGCCTGTCTACTCCTCAGCCAACAGCACAAATAACTGTATCATA TCCAACACCAAGGTCCAGTCAACAGCAAACCCAGCCTCAGAAGCAGCGTGTTTTCACAGGGGTGGTTACA AAACTACATGATACGTTTGGATTTGTGGATGAAGATGTATTCTTTCAGCTTAGTGCTGTCAAAGGGAAAA CCCCCCAAGTAGGTGACAGAGTATTGGTTGAAGCTACTTATAATCCTAATATGCCTTTTAAATGGAATGC ACAGAGAATTCAAACACTACCAAATCAGAATCAGTCGCAAACCCAGCCATTACTGAAGACTCCTCCTGCT GTACTTCAGCCAATTGCACCACAGACAACATTTGGTGTTCAGACTCAGCCCCAGCCCCAGTCACTGCTGC AGGCACAGATTTCAGCAGCTTCTATTACACCACTATTGCAGACTCAACCACAGCCCTTATTACAGCAGCC TCAGCAAAAAGCTGGTTTATTGCAGCCTCCTGTTCGTATAGTTTCACAGCCACAACCGGCACGACGATTA GATCCCCCATCCCGATTTTCAGGAAGAAATGACAGAGGGGATCAAGTGCCTAACAGAAAAGATGATCGAA GTCGTGAGAGAGAGAGAGAAAGACGTAGATCGAGAGAAAGATCACCTCAGAGGAAACGTTCCCGGGAAAG ATCTCCACGAAGAGAGCGAGAGCGATCACCTCGGAGAGTTCGACGTGTTGTTCCACGTTACACAGTTCAG TTTTCAAAGTTTTCTTTAGATTGTCCCAGTTGTGACATGATGGAACTAAGGCGCCGTTATCAAAATTTGT ATATACCTAGTGACTTTTTTGATGCTCAATTTACATGGGTGGATGCTTTCCCTTTGTCAAGACCATTTCA GCTGGGAAATTACTGCAATTTTTATGTAATGCACAGAGAAGTAGAGTCCTTAGAAAAAAATATGGCCATT CTTGATCCACCAGATGCTGACCACTTATACAGTGCAAAGGTAATGCTGATGGCTAGCCCTAGTATGGAAG ATTTATATCATAAGTCATGTGCTCTTGCTGAGGACCCACAAGAACTTCGAGATGGATTCCAACATCCTGC TAGACTTGTTAAGTTTTTAGTGGGCATGAAAGGCAAGGATGAAGCTATGGCCATTGGAGGCCACTGGTCT CCTTCGTTGGATGGACCAGACCCAGAAAAAGATCCCTCTGTGTTGATTAAGACTGCTATTCGTTGTTGTA AGGCTCTGACAGGCATTGATCTAAGTGTGTGCACACAATGGTACCGTTTTGCAGAGATTCGCTACCATCG CCCTGAGGAGACCCACAAGGGGCGTACAGTTCCAGCTCATGTGGAGACAGTGGTTTTATTTTTCCCGGAT GTTTGGCATTGCCTTCCCACCCGCTCAGAGTGGGAAACCCTCTCCCGAGGATACAAGCAGCAGCTGGTCG AGAAGCTTCAGGGTGAACGCAAGGAGGCTGATGGAGAACAGGATGAAGAAGAGAAGGATGATGGTGAAGC TAAAGAAATTTCTACACCTACCCATTGGTCTAAACTTGATCCAAAGACAATGAAGGTAAATGACCTCCGA AAAGAATTAGAAAGTCGAGCTCTTAGTTCCAAAGGATTAAAATCCCAGTTAATAGCCCGATTGACAAAAC AGCTTAAAGTAGAGGAACAAAAAGAAGAACAGAAGGAGTTAGAGAAATCTGAAAAAGAAGAGGATGAGGA TGATGATAGGAAATCTGAAGACGATAAAGAGGAAGAAGAAAGGAAACGTCAAGAGGAAATAGAACGCCAG CGTCGAGAAAGAAGATATATTTTGCCTGATGAACCGGCCATCATTGTACATCCAAATTGGGCTGCAAAAA GTGGCAAGTTTGATTGTAGCATCATGTCTTTGAGTGTCCTATTGGACTACAGATTAGAGGATAATAAAGA ACATTCATTTGAGGTTTCATTGTTTGCGGAACTTTTCAACGAAATGCTTCAAAGAGATTTTGGTGTCCGT ATATACAAATCATTACTGTCTCTTCCTGAGAAAGAGGACAAAAAAGAAAAGGATAAAAAAAGCAAAAAAG ATGAGAGAAAAGATAAAAAAGAAGAAAGAGATGATGAAACTGATGAACCAAAACCCAAACGGAGAAAATC AGGCGATGATAAAGATAAAAAAGAAGATAGAGATGAAAGGAAGAAAGAAGATAAAAGAAAAGATGATTCT AAAGATGATGATGAAACTGAAGAAGATAACAATCAAGATGAATATGACCCTATGGAAGCAGAAGAAGCTG AGGATGAAGAAGATGATAGGGATGAGGAAGAAATGACCAAACGAGATGACAAAAGAGATATCAACAGATA CTGCAAGGAGAGGCCCTCTAAAGATAAGGAAAAAGAAAAGACTCAAATGATCACAATTAACAGAGATCTG TTAATGGCTTTTGTTTATTTTGATCAAAGTCATTGTGGTTACCTTCTTGAAAAGGATTTGGAAGAAATAC TTTATACTCTTGGACTACATCTTTCTCGGGCTCAGGTAAAGAAGCTTCTTAATAAAGTAGTGCTCCGTGA ATCTTGCTTTTACCGGAAATTAACAGACACCTCAAAAGATGAAGAGAACCATGAAGAGTCTGAGTCATTG CAGGAAGATATGCTAGGAAACAGATTATTACTTCCAACACCAACAGTAAAGCAGGAATCAAAGGATGTGG AAGAAAATGTTGGCCTCATTGTGTACAATGGTGCAATGGTAGATGTAGGAAGCCTCTTGCAAAAATTGGA AAAGAGCGAAAAAGTAAGAGCTGAGGTAGAACAGAAGCTGCAGTTACTAGAAGAAAAAACAGATGAAGAT GAAAAAACCATATTAAATTTGGAGAATTCCAACAAAAGCCTCTCTGGTGAACTCAGAGAAGTTAAAAAGG ACCTTAGTCAGTTACAAGAAAACTTAAAGATTTCGGAAAACATGAATTTACAATTTGAAAACCAAATGAA TAAGACAATCAGAAACTTATCTACGGTAATGGATGAAATCCACACTGTTCTCAAGAAGGATAATGTAAAG AATGAAGACAAAGATCAAAAATCCAAGGAGAATGGTGCCAGTGTATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282959
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282959.1, NP_001269888.1
RefSeq Size	4730 bp
RefSeq ORF	3408 bp
Locus ID	55749
UniProt ID	Q8IX12
Gene Summary	Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (PubMed:12816952). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Variants 2 and 3 encode the same isoform (b).

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