MCAF1 (ATF7IP) (NM_001286514) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AM; ATF-IP; MCAF; MCAF1; p621
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286514, the custom clone sequence may differ by one or more nucleotides ATGGACAGTTTAGAAGAACCTCAGAAAAAAGTCTTTAAGGCTCGAAAAACGATGAGAGTGAGTGATCGTC AGCAACTTGAAGCAGTGTACAAGGTCAAAGAAGAACTGTTGAAAACTGATGTCAAGCTGTTAAATGGCAA CCATGAAAATGGAGATTTGGACCCAACCTCACCTTTGGAAAACATGGATTACATTAAAGACAAGGAAGAG GTGAATGGCATTGAAGAGATTTGTTTTGATCCTGAAGGAAGTAAAGCAGAATGGAAGGAAACACCCTGTA TCCTAAGTGTTAATGTAAAAAACAAGCAGGATGATGATTTAAATTGTGAACCTTTGTCTCCCCATAATAT AACTCCAGAACCAGTCTCTAAACTGCCTGCTGAACCAGTTTCTGGTGATCCAGCCCCTGGTGATCTGGAT GCCGGAGATCCAGCCTCCGGAGTACTGGCCTCTGGTGATTCCACCTCTGGTGATCCCACCTCTAGCGAGC CCTCCTCTAGTGATGCTGCCTCTGGTGATGCAACCTCTGGTGATGCCCCTTCTGGTGATGTGTCCCCTGG TGATGCCACCTCTGGTGATGCCACTGCTGATGATCTCTCCTCTGGTGATCCCACCTCTAGTGATCCCATC CCAGGTGAACCGGTCCCTGTTGAACCCATTTCTGGTGATTGTGCCGCTGATGATATAGCCTCTAGTGAAA TAACTTCTGTTGATCTGGCTTCTGGAGCACCAGCTTCCACTGATCCAGCCTCTGATGATCTGGCCTCTGG TGATCTATCCTCTAGTGAACTGGCCTCTGATGATCTGGCCACTGGTGAACTGGCCTCTGATGAGCTGACT TCTGAATCAACCTTTGATCGTACCTTTGAACCAAAGTCTGTACCAGTTTGTGAACCAGTTCCTGAAATTG ACAATATAGAACCAAGTAGCAATAAAGATGATGATTTTCTTGAAAAAAATGGAGCTGATGAAAAATTAGA GCAAATTCAGAGTAAAGACTCATTGGATGAGAAAAATAAAGCTGATAATAATATTGATGCTAATGAAGAA ACTCTAGAAACAGATGATACAACTATTTGTTCAGATCGACCTCCTGAAAATGAAAAGAAGGTAGAGGAAG ATATTATCACAGAGCTTGCTCTTGGAGAAGATGCTATATCTAGCAGTATGGAAATTGACCAAGGTGAAAA GAATGAAGATGAAACTTCTGCAGATCTTGTAGAAACGATTAATGAAAATGTTATTGAAGATAACAAAAGT GAGAATATCTTAGAAAATACAGACTCTATGGAGACAGATGAAATCATTCCTATTTTGGAAAAGCTTGCAC CTTCTGAGGATGAACTTACTTGCTTTTCTAAAACATCTCTCCTTCCAATCGATGAGACAAATCCAGATTT GGAAGAGAAAATGGAAAGTTCTTTTGGTTCACCATCTAAACAAGAAAGTAGTGAGAGTTTGCCAAAAGAA GCCTTTCTGGTCCTCTCTGATGAAGAGGATATTTCGGGTGAAAAAGATGAGTCTGAAGTTATATCGCAAA ATGAAACGTGCTCTCCAGAAGTAGAAAGTAATGAAAAGGACAACAAACCTGAGGAAGAAGAGCAAGTAAT ACATGAAGATGATGAAAGACCTTCTGAGAAAAATGAATTTTCTAGACGAAAACGTTCTAAATCAGAAGAC ATGGACAATGTACAGTCTAAACGTCGTCGATATATGGAAGAAGAATATGAGGCAGAATTTCAAGTAAAGA TTACAGCCAAAGGAGACATTAACCAGAAACTTCAAAAGGTTATACAGTGGTTGCTGGAAGAAAAATTGTG TGCGCTGCAGTGTGCTGTATTTGATAAGACTTTGGCAGAATTGAAAACACGAGTGGAAAAGATTGAATGT AACAAGAGGCATAAAACAGTTCTCACTGAACTACAGGCCAAGATAGCCAGGTTAACCAAACGCTTTGAAG CAGCCAAAGAAGATCTTAAGAAAAGACATGAACATCCACCCAACCCACCAGTATCACCAGGAAAAACTGT AAATGATGTCAACAGCAATAATAACATGTCTTACAGAAATGCAGGCACAGTGAGACAGATGCTGGAGTCC AAAAGAAATGTAAGCGAGAGTGCACCACCATCCTTTCAAACTCCTGTGAATACAGTATCTTCAACCAATC TTGTCACTCCTCCAGCAGTTGTCAGTAGTCAACCTAAATTGCAGACTCCAGTGACTTCGGGTTCCCTCAC AGCAACGTCAGTTCTTCCTGCACCCAATACAGCTACTGTAGTTGCTACTACTCAGGTGCCTAGTGGAAAT CCCCAGCCTACAATCTCTTTACAGCCTTTGCCAGTGATTTTGCATGTACCTGTTGCAGTATCCTCCCAGC CTCAGCTTCTACAGAGCCATCCAGGGACTTTGGTGACTAATCAACCATCTGGCAATGTTGAATTCATTTC TGTGCAAAGCCCACCTACAGTGAGTGGTCTTACCAAAAATCCAGTATCCTTGCCATCCTTGCCAAATCCC ACTAAACCAAACAACGTTCCTTCTGTGCCCAGTCCTAGTATTCAAAGGAACCCTACTGCCAGTGCTGCAC CATTGGGAACAACACTTGCTGTGCAGGCTGTTCCAACAGCACACTCTATTGTACAAGCCACAAGGACTTC TTTACCCACAGTGGGCCCATCAGGACTCTATAGTCCATCAACTAATCGAGGTCCTATACAGATGAAAATT CCAATTTCTGCATTTAGTACTTCGTCTGCTGCAGAACAGAACAGCAATACCACCCCAAGAATTGAAAACC AGACAAACAAAACAATAGATGCTTCTGTCAGTAAGAAAGCAGCTGATAGCACATCACAGTGTGGAAAAGC CACTGGCAGTGATTCAAGTGGTGTCATTGATCTCACAATGGATGATGAAGAGAGTGGAGCTTCACAAGAC CCCAAAAAACTAAATCACACTCCTGTATCAACCATGAGTTCTTCTCAGCCTGTGTCACGACCATTGCAAC CCATACAACCAGCACCGCCTCTTCAACCATCTGGGGTGCCAACAAGTGGACCATCTCAGACCACCATACA CTTACTACCTACAGCTCCAACTACCGTGAATGTAACACATCGTCCAGTAACTCAGGTGACCACAAGACTC CCTGTACCAAGAGCTCCTGCAAACCACCAGGTGGTTTATACAACTCTTCCTGCACCACCAGCTCAGGCTC CCTTGCGAGGAACTGTTATGCAGGCTCCTGCTGTTCGGCAGGTCAATCCCCAAAATAGTGTTACAGTTCG AGTGCCTCAAACAACCACATATGTTGTAAACAATGGACTAACCCTGGGATCAACAGGACCTCAGCTCACA GTGCATCACCGACCACCACAAGTGCATACTGAGCCCCCACGCCCCGTGCACCCAGCACCCTTACCAGAAG CTCCACAACCACAGCGTCTGCCCCCAGAAGCTGCCAGCACATCTCTGCCTCAGAAGCCACACTTGAAGTT AGCACGCGTTCAGAGTCAAAATGGCATAGTACTGTCATGGAGTGTCCTGGAGGTGGATCGAAGCTGTGCC ACTGTTGATAGCTACCATCTCTATGCTTACCATGAGGAACCCAGTGCCACTGTGCCCTCACAATGGAAAA AGATTGGGGAAGTCAAGGCACTTCCCTTGCCCATGGCATGTACTCTCACCCAGTTTGTATCTGGTAGCAA ATACTACTTTGCAGTACGAGCCAAGGATATTTATGGACGTTTTGGGCCTTTCTGTGATCCTCAGTCAACA GATGTGATCTCTTCTACCCAGAGCAGTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001286514
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286514.1, NP_001273443.1
RefSeq Size	8875 bp
RefSeq ORF	3810 bp
Locus ID	55729
UniProt ID	Q6VMQ6
Protein Families	Transcription Factors
Gene Summary	ATF7IP is a multifunctional nuclear protein that associates with heterochromatin. It can act as a transcriptional coactivator or corepressor depending upon its binding partners (summary by Liu et al., 2009 [PubMed 19106100]).[supplied by OMIM, Nov 2010] Transcript Variant: This variant (3) differs in the 5' UTR and contains multiple differences in the 5' coding region, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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