NUP155 (NM_001278312) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATFB15; N155
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278312, the custom clone sequence may differ by one or more nucleotides ATGCCGTCTTCTTTGTTGGGCGCGGCGATGCCGGCCTCTACATCTGCCGCAGCCCTGCAGGAAGCTCTGG AAAATGCTGGACGGCTCATCGACCGTCAGTTGCAAGAGGACCGCATGTACCCGGACCTTTCCGAGCTGCT TATGGTGTCTGCCCCAAATAATCCCACCGTTTCTGGCATGTCAGATATGGATTATCCTTTGCAAGGACCT GGTTTGCTGTCCGTACCCAACCTTCCAGAGATCAGTTCCATCCGAAGAGTTCCTCTCCCACCTGAACTTG TTGAGCAGTTTGGACATATGCAGTGTAATTGCATGATGGGTGTGTTCCCTCCTATCAGCAGAGCTTGGCT CACAATTGACAGTGATATATTCATGTGGAACTATGAGGATGGAGGAGACCTTGCCTATTTTGATGGACTT AGTGAGACTATTCTTGCTGTGGGGCTTGTGAAGCCAAAAGCAGGCATCTTTCAACCTCATGTGCGACACC TCCTGGTTTTGGCGACCCCTGTAGACATAGTAATTCTTGGACTCAGCTATGCTAATTTGCAAACAGGTTC TGGAGTTCTTAATGATAGTTTGTCTGGTGGAATGCAGTTGCTTCCAGATCCTTTATATTCTCTTCCTACT GATAATACTTACCTTTTAACAATAACTTCCACTGATAATGGCAGAATTTTCTTGGCTGGAAAGGATGGCT GTTTATATGAAGTAGCCTACCAGGCTGAAGCAGGGTGGTTTAGCCAAAGATGTAGGAAAATAAACCACTC AAAGAGCTCACTTTCTTTCCTTGTTCCTTCCTTGCTACAATTCACGTTCTCAGAAGATGATCCTATTCTT CAAATTGCAATTGATAATTCTAGAAATATTTTATATACACGATCTGAGAAAGGAGTAATACAGGTGTATG ATTTGGGACAAGATGGACAAGGAATGAGCAGAGTTGCCTCTGTGTCACAGAATGCCATTGTCTCTGCTGC TGGTAACATTGCTAGGACCATCGATCGTTCTGTTTTTAAACCAATTGTCCAAATAGCAGTGATTGAAAAT TCTGAATCACTGGACTGTCAGTTATTGGCTGTCACACATGCAGGTGTTAGGTTATATTTTAGCACTTGTC CATTCAGACAGCCATTAGCACGGCCTAATACACTGACGCTGGTTCATGTCCGCTTACCTCCTGGATTCTC AGCATCTTCAACCGTGGAAAAGCCTTCAAAAGTACATAGAGCTCTTTATAGTAAAGGTATTCTATTGATG GCAGCCTCAGAAAATGAGGATAATGATATTTTATGGTGTGTCAACCATGATACTTTTCCTTTCCAAAAGC CAATGATGGAAACCCAGATGACAGCTGGTGTTGATGGTCATTCCTGGGCTCTTTCTGCGATAGATGAATT GAAAGTAGATAAAATAATTACACCTTTAAACAAGGATCATATTCCAATAACTGATTCACCAGTTGTTGTA CAGCAGCACATGTTACCTCCGAAGAAATTTGTTCTCCTCTCAGCACAGGGGAGCCTTATGTTTCATAAAC TTAGACCTGTAGATCAACTGAGGCATCTACTTGTGAGTAATGTGGGAGGAGATGGAGAAGAGATTGAAAG ATTCTTTAAATTACATCAGGAAGACCAGGCTTGTGCAACTTGCCTTATTCTTGCTTGCTCCACTGCTGCC TGTGATAGAGAAGTATCTGCCTGGGCTACTCGGGCTTTCTTTAGGTATGGTGGTGAAGCACAGATGAGAT TTCCAACCACTCTTCCGCCTCCAAGTAATGTTGGTCCCATCTTGGGGTCTCCTGTCTATTCTAGTTCTCC TGTTCCTAGTGGTAGTCCCTATCCAAATCCATCCTTTTTGGGAACACCGTCTCATGGTATACAGCCTCCT GCCATGTCAACTCCAGTGTGTGCTCTGGGAAACCCAGCAACTCAGGCCACAAATATGAGTTGTGTGACTG GACCAGAGATTGTGTACTCTGGAAAACACAATGGTATTTGCATTTACTTTTCTCGGATCATGGGAAACAT TTGGGATGCAAGCTTAGTTGTGGAGAGAATATTCAAGAGTGGCAACAGAGAGATCACTGCAATTGAAAGT AGTGTTCCCTGCCAACTGCTAGAGTCAGTGCTACAAGAACTAAAGGGTTTGCAGGAATTTCTAGACAGAA ACTCCCAGTTTGCAGGAGGACCATTAGGAAATCCAAATACTACTGCTAAAGTGCAGCAGAGGCTGATAGG ATTCATGCGTCCTGAAAACGGAAATCCCCAGCAAATGCAACAGGAACTGCAGAGGAAGTTTCATGAGGCT CAACTAAGTGAAAAGATTTCACTTCAGGCAATTCAGCAGTTGGTTCGAAAATCATATCAGGCTCTGGCTT TATGGAAACTTCTTTGTGAACATCAATTCACTATCATTGTGGCAGAACTTCAGAAGGCAAATGAGCTTCT CCAGCGTTCCCGACAAGTTCAAAATAAGACTGAAAAAGAAAGAATGTTAAGGGAATCATTAAAGGAATAT CAAAAAATTAGCAATCAAGTGGACCTTTCCAATGTTTGTGCTCAGTATAGACAAGTGAGATTTTATGAGG GTGTGGTGGAACTTTCTCTTACGGCTGCAGAGAAAAAAGATCCTCAAGGTCTTGGGCTTCATTTCTATAA ACATGGAGAACCAGAAGAAGACATAGTTGGACTTCAGGCCTTCCAAGAAAGATTAAACAGTTACAAATGC ATTACAGACACACTTCAAGAACTGGTAAATCAAAGTAAGGCCGCTCCTCAGTCTCCCAGTGTACCCAAAA AACCTGGTCCTCCAGTGTTGTCATCTGATCCAAATATGCTGAGTAATGAAGAAGCAGGACATCATTTTGA ACAAATGCTTAAATTGTCACAGCGATCCAAGGATGAGCTCTTTAGTATTGCCCTTTATAATTGGCTAATA CAAGTCGACCTTGCAGATAAGCTGCTACAGGTTGCTTCTCCATTTCTGGAGCCACATCTAGTCCGAATGG CCAAAGTTGATCAAAACAGAGTTCGTTATATGGATTTACTCTGGCGGTATTACGAGAAGAACAGAAGTTT CAGTAATGCTGCTCGTGTACTGTCCAGACTGGCTGACATGCATAGCACAGAAATTTCACTTCAGCAGCGA CTAGAGTACATTGCTCGAGCCATTCTTAGTGCCAAAAGTTCCACTGCCATTTCATCAATAGCTGCCGATG GTGAATTTCTTCATGAATTAGAAGAAAAAATGGAGGTTGCTAGGATCCAACTTCAGATACAGGAGACACT ACAAAGGCAGTATTCCCATCATTCTTCTGTACAGGATGCAGTTTCTCAGCTGGATTCTGAGCTGATGGAC ATAACTAAGCTTTATGGGGAATTTGCTGACCCATTTAAACTTGCAGAGTGCAAACTTGCAATAATTCATT GTGCCGGTTATTCAGACCCTATATTGGTGCAGACACTTTGGCAAGATATCATAGAGAAAGAATTGAGTGA CAGTGTGACATTGAGCTCCTCGGATAGAATGCATGCTCTTAGTCTCAAGATTGTTCTCCTTGGCAAAATT TATGCTGGCACACCACGCTTCTTTCCTTTAGATTTTATTGTACAGTTTTTAGAACAGCAGGTTTGTACTT TGAACTGGGATGTGGGCTTCGTAATACAGACAATGAATGAAATTGGAGTACCATTACCTAGACTACTAGA AGTTTATGATCAGTTGTTCAAATCACGGGATCCATTCTGGAACAGAATGAAGAAGCCACTGCACCTTTTG GATTGTATACATGTATTATTGATAAGATATGTTGAGAATCCCAGCCAAGTTTTAAATTGTGAAAGGAGAA GATTTACAAATCTCTGCCTGGATGCTGTTTGTGGTTACCTTGTTGAGCTCCAGTCTATGAGCTCGTCAGT AGCAGTACAAGCCATCACTGGGAATTTTAAATCTCTTCAAGCTAAATTAGAACGGCTTCATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001278312
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278312.1, NP_001265241.1
RefSeq Size	4408 bp
RefSeq ORF	3984 bp
Locus ID	9631
Gene Summary	Nucleoporins are proteins that play an important role in the assembly and functioning of the nuclear pore complex (NPC) which regulates the movement of macromolecules across the nuclear envelope (NE). The protein encoded by this gene plays a role in the fusion of NE vesicles and formation of the double membrane NE. The protein may also be involved in cardiac physiology and may be associated with the pathogenesis of atrial fibrillation. Alternative splicing results in multiple transcript variants of this gene. A pseudogene associated with this gene is located on chromosome 6. [provided by RefSeq, May 2013] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 3 which lacks an internal segment and is shorter, compared to isoform 1.

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