TRPM7 (NM_001301212) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ALSPDC; CHAK; CHAK1; LTrpC-7; LTRPC7; TRP-PLIK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301212, the custom clone sequence may differ by one or more nucleotides ATGTCCCAGAAATCCTGGATAGAAAGCACTTTGACCAAGAGGGAATGTGTATATATTATACCAAGTTCCA AGGACCCTCACAGATGCCTTCCAGGATGTCAAATTTGTCAGCAACTCGTCAGGTGTTTTTGTGGTCGCTT GGTCAAGCAACATGCTTGTTTTACTGCAAGTCTTGCCATGAAATACTCAGATGTGAAATTGGGTGACCAT TTTAATCAGGCAATAGAAGAATGGTCTGTGGAAAAGCATACAGAACAGAGCCCAACGGATGCTTATGGAG TCATAAATTTTCAAGGGGGTTCTCATTCCTACAGAGCTAAGTATGTGAGGCTATCATATGACACCAAACC TGAAGTCATTCTGCAACTTCTGCTTAAAGAATGGCAAATGGAGTTACCCAAACTTGTTATCTCTGTACAT GGGGGCATGCAGAAATTTGAGCTTCACCCACGAATCAAGCAGTTGCTTGGAAAAGGTCTTATTAAAGCTG CAGTTACAACTGGAGCCTGGATTTTAACTGGAGGAGTAAACACAGGTGTGGCAAAACATGTTGGAGATGC CCTCAAAGAACATGCTTCCAGATCATCTCGAAAGATTTGCACTATCGGAATAGCTCCATGGGGAGTGATT GAAAACAGAAATGATCTTGTTGGGAGAGATGTGGTTGCTCCTTATCAAACCTTATTGAACCCCCTGAGCA AATTGAATGTTTTGAATAATCTGCATTCCCATTTCATATTGGTGGATGATGGCACTGTTGGAAAGTATGG GGCGGAAGTCAGACTGAGAAGAGAACTTGAAAAAACTATTAATCAGCAAAGAATTCATGCTAGGATTGGC CAGGGTGTCCCTGTGGTGGCACTTATATTTGAGGGTGGGCCAAATGTTATCCTCACAGTTCTTGAATACC TTCAGGAAAGCCCCCCTGTTCCAGTAGTTGTGTGTGAAGGAACAGGCAGAGCTGCAGATCTGCTAGCGTA TATTCATAAACAAACAGAAGAAGGAGGGAATCTTCCTGATGCAGCAGAGCCCGATATTATTTCCACTATC AAAAAAACATTTAACTTTGGCCAGAATGAAGCACTTCATTTATTTCAAACACTGATGGAGTGCATGAAAA GAAAGGAGCTTATCACTGTTTTCCATATTGGGTCAGATGAACATCAAGATATAGATGTAGCAATACTTAC TGCACTGCTAAAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACATTGGCATGGGATAGAGTT GACATTGCCAAAAATCATGTATTTGTTTATGGACAGCAGTGGCTGGTTGGATCCTTGGAACAAGCTATGC TTGATGCTCTTGTAATGGATAGAGTTGCATTTGTAAAACTTCTTATTGAAAATGGAGTAAGCATGCATAA ATTCCTTACCATTCCGAGACTGGAAGAACTTTACAACACTAAACAAGGTCCAACTAATCCAATGCTGTTT CATCTTGTTCGAGACGTCAAACAGGGAAATCTTCCTCCAGGATATAAGATCACTCTGATTGATATAGGAC TTGTTATTGAATATCTCATGGGAGGAACCTACAGATGCACCTATACTAGGAAACGTTTTCGATTAATATA TAATAGTCTTGGTGGAAATAATCGGAGGTCTGGCCGAAATACCTCCAGCAGCACTCCTCAGTTGCGAAAG AGTCATGAATCTTTTGGCAATAGGGCAGATAAAAAGGAAAAAATGAGGCATAACCATTTCATTAAGACAG CACAGCCCTACCGACCAAAGATTGATACAGTTATGGAAGAAGGAAAGAAGAAAAGAACCAAAGATGAAAT TGTAGACATTGATGATCCAGAAACCAAGCGCTTTCCTTATCCACTTAATGAACTTTTAATTTGGGCTTGC CTTATGAAGAGGCAGGTCATGGCCCGTTTTTTATGGCAACATGGTGAAGAATCAATGGCTAAAGCATTAG TTGCCTGTAAGATCTATCGTTCAATGGCATATGAAGCAAAGCAGAGTGACCTGGTAGATGATACTTCAGA AGAACTAAAACAGTATTCCAATGATTTTGGTCAGTTGGCCGTTGAATTATTAGAACAGTCCTTCAGACAA GATGAAACCATGGCTATGAAATTGCTCACTTATGAACTGAAGAACTGGAGTAATTCAACCTGCCTTAAGT TAGCAGTTTCTTCAAGACTTAGACCTTTTGTAGCTCACACCTGTACACAAATGTTGTTATCTGATATGTG GATGGGAAGGCTGAATATGAGGAAAAATTCCTGGTACAAGGTCATACTAAGCATTTTAGTTCCACCTGCC ATATTGCTGTTAGAGTATAAAACTAAGGCTGAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAGA TGACAATGGATGACAGCGAAAACAACTTTCAGAACATAACAGAAGAGATCCCCATGGAAGTGTTTAAAGA AGTACGGATTTTGGATAGTAATGAAGGAAAGAATGAGATGGAGATACAAATGAAATCAAAAAAGCTTCCA ATTACGCGAAAGTTTTATGCCTTTTATCATGCACCAATTGTAAAATTCTGGTTTAACACGTTGGCATATT TAGGATTTCTGATGCTTTATACATTTGTGGTTCTTGTACAAATGGAACAGTTACCTTCAGTTCAAGAATG GATTGTTATTGCTTATATTTTTACTTATGCCATTGAGAAAGTCCGTGAGATCTTTATGTCTGAAGCTGGG AAAGTAAACCAGAAGATTAAAGTATGGTTTAGTGATTACTTCAACATCAGTGATACAATTGCCATAATTT CTTTCTTCATTGGATTTGGACTAAGATTTGGAGCAAAATGGAACTTTGCAAATGCATATGATAATCATGT TTTTGTGGCTGGAAGATTAATTTACTGTCTTAACATAATATTTTGGTATGTGCGTTTGCTAGATTTTCTA GCTGTAAATCAACAGGCAGGACCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATGTTCTACATTG TAGTGATTATGGCTCTTGTATTACTTAGTTTTGGTGTTCCCAGAAAGGCAATACTTTATCCTCATGAAGC ACCATCTTGGACTCTTGCTAAAGATATAGTTTTTCACCCATACTGGATGATTTTTGGTGAAGTTTATGCA TACGAAATTGATGTGTGTGCAAATGATTCTGTTATCCCTCAAATCTGTGGTCCTGGGACGTGGTTGACTC CATTTCTTCAAGCAGTCTACCTCTTTGTACAGTATATCATTATGGTTAATCTTCTTATTGCATTTTTCAA CAATGTGTATTTACAAGTGAAGGCAATTTCCAATATTGTATGGAAGTACCAGCGTTATCATTTTATTATG GCTTATCATGAGAAACCAGTTCTGCCTCCTCCACTTATCATTCTTAGCCATATAGTTTCTCTGTTTTGCT GCATATGTAAGAGAAGAAAGAAAGATAAGACTTCCGATGGACCAAAACTTTTCTTAACAGAAGAAGATCA AAAGAAACTTCATGATTTTGAAGAGCAGTGTGTTGAAATGTATTTCAATGAAAAAGATGACAAATTTCAT TCTGGGAGTGAAGAGAGAATTCGTGTCACTTTTGAAAGAGTGGAACAGATGTGCATTCAGATTAAAGAAG TTGGAGATCGTGTCAACTACATAAAAAGATCATTACAATCATTAGATTCTCAAATTGGCCATTTGCAAGA TCTTTCAGCCCTGACGGTAGATACATTAAAAACACTCACTGCCCAGAAAGCGTCGGAAGCTAGCAAAGTT CATAATGAAATCACACGAGAACTGAGCATTTCCAAACACTTGGCTCAAAACCTTATTGATGATGGTCCTG TAAGACCTTCTGTATGGAAAAAGCATGGTGTTGTAAATACACTTAGCTCCTCTCTTCCTCAAGGTGATCT TGAAAGTAATAATCCTTTTCATTGTAATATTTTAATGAAAGATGACAAAGATCCCCAGTGTAATATATTT GGTCAAGACTTACCTGCAGTACCCCAGAGAAAAGAATTTAATTTTCCAGAGGCTGGTTCCTCTTCTGGTG CCTTATTCCCAAGTGCTGTTTCCCCTCCAGAACTGCGACAGAGACTACATGGGGTAGAACTCTTAAAAAT ATTTAATAAAAATCAAAAATTAGGCAGTTCATCTACTAGCATACCACATCTGTCATCCCCACCAACCAAA TTTTTTGTTAGTACACCATCTCAGCCAAGTTGCAAAAGCCACTTGGAAACTGGAACCAAAGATCAAGAAA CTGTTTGCTCTAAAGCTACAGAAGGAGATAATACAGAATTTGGAGCATTTGTAGGACACAGAGATAGCAT GGATTTACAGAGGTTTAAAGAAACATCAAACAAGATAAAAATACTATCCAATAACAATACTTCTGAAAAC ACTTTGAAACGAGTGAGTTCTCTTGCTGGATTTACTGACTGTCACAGAACTTCCATTCCTGTTCATTCAA AACAAGAAAAAATCAGTAGAAGGCCATCTACCGAAGACACTCATGAAGTAGATTCCAAAGCAGCTTTAAT ACCGGATTGGTTACAAGATAGACCATCAAACAGAGAAATGCCATCTGAAGAAGGAACATTAAATGGTCTC ACTTCTCCATTTAAGCCAGCTATGGATACAAATTACTATTATTCAGCTGTGGAAAGAAATAACTTGATGA GGTTATCACAGAGCATTCCATTTACACCTGTGCCTCCAAGAGGGGAGCCTGTCACAGTGTATCGTTTGGA AGAGAGTTCACCCAACATACTAAATAACAGCATGTCTTCTTGGTCACAACTAGGCCTCTGTGCCAAAATA GAGTTTTTAAGCAAAGAGGAGATGGGAGGAGGTTTACGAAGAGCTGTCAAAGTACAGTGTACCTGGTCAG AACATGATATCCTCAAATCAGGGCATCTTTATATTATCAAATCTTTTCTTCCAGAGGTGGTTAATACATG GTCAAGTATTTACAAAGAAGATACAGTTCTGCATCTCTGTCTGAGAGAAATTCAACAACAGAGAGCAGCA CAAAAGCTTACGTTTGCCTTTAATCAAATGAAACCCAAATCCATACCATATTCTCCAAGGTTCCTTGAAG TTTTCCTGCTGTATTGCCATTCAGCAGGACAGTGGTTTGCTGTGGAAGAATGTATGACTGGAGAATTTAG AAAATACAACAATAATAATGGAGATGAGATTATTCCAACTAATACTCTGGAAGAGATCATGCTAGCCTTT AGCCACTGGACTTACGAATATACAAGAGGGGAGTTACTGGTACTTGATTTGCAAGGTGTTGGTGAAAATT TGACTGACCCATCTGTGATAAAAGCAGAAGAAAAGAGATCCTGTGATATGGTTTTTGGCCCAGCAAATCT AGGAGAAGATGCAATTAAAAACTTCAGAGCAAAACATCACTGTAATTCTTGCTGTAGAAAGCTTAAACTT CCAGATCTGAAGAGGAATGATTATACGCCTGATAAAATTATATTTCCTCAGGATGAGCCTTCAGATTTGA ATCTTCAGCCTGGAAATTCCACCAAAGAATCAGAATCAACTAATTCTGTTCGTCTGATGTTATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301212
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301212.1, NP_001288141.1
RefSeq Size	10409 bp
RefSeq ORF	5595 bp
Locus ID	54822
UniProt ID	Q96QT4
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Protein Kinase, Transmembrane
Gene Summary	This gene belongs to the melastatin subfamily of transient receptor potential family of ion channels. The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. The encoded protein is involved in cytoskeletal organization, cell adhesion, cell migration and organogenesis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam. The gene may also be associated with defects of cardiac function. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is 1 aa shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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