CHD4 (NM_001297553) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CHD-4; Mi-2b; Mi2-BETA; SIHIWES
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001297553, the custom clone sequence may differ by one or more nucleotides ATGGCGTCGGGCCTGGGCTCCCCGTCCCCCTGCTCGGCGGGCAGTGAGGAGGAGGATATGGATGCACTTT TGAACAACAGCCTGCCCCCACCCCACCCAGAAAATGAAGAGGACCCAGAAGAGGATTTGTCAGAAACAGA GACTCCAAAGCTCAAGAAGAAGAAAAAGCCTAAGAAACCTCGGGACCCTAAAATCCCTAAGAGCAAGCGC CAAAAAAAGGAGCTGGGGGACAGCTCTGGGGAGGGGCCAGAGTTTGTGGAGGAGGAGGAAGAGGTGGCTC TGCGCTCAGACAGTGAGGGCAGCGACTATACTCCTGGCAAGAAGAAGAAGAAGAAGCTTGGACCTAAGAA AGAGAAGAAGAGCAAATCCAAGCGGAAGGAGGAGGAGGAGGAGGAGGATGATGATGATGATTCAAAGGAG CCTAAATCATCTGCTCAGCTCCTGGAAGACTGGGGCATGGAAGACATTGACCACGTGTTCTCAGAGGAGG ATTATCGAACCCTCACCAACTACAAGGCCTTCAGCCAGTTTGTCAGACCCCTCATTGCTGCCAAAAATCC CAAGATTGCTGTCTCCAAGATGATGATGGTTTTGGGTGCAAAATGGCGGGAGTTCAGTACCAATAACCCC TTCAAAGGCAGTTCTGGGGCATCAGTGGCAGCTGCGGCAGCAGCAGCGGTAGCTGTGGTGGAGAGCATGG TGACAGCCACTGAGGTTGCACCACCACCTCCCCCTGTGGAGGTGCCTATCCGCAAGGCCAAGACCAAGGA GGGCAAAGGTCCCAATGCTCGGAGGAAGCCCAAGGGCAGCCCTCGTGTACCTGATGCCAAGAAGCCTAAA CCCAAGAAAGTAGCTCCCCTGAAAATCAAGCTGGGAGGTTTTGGTTCCAAGCGTAAGAGATCCTCGAGTG AGGATGATGACTTAGATGTGGAATCTGACTTCGATGATGCCAGTATCAATAGCTATTCTGTTTCTGATGG TTCCACCAGCCGTAGTAGCCGCAGCCGCAAGAAACTCCGAACCACTAAAAAGAAAAAGAAAGGCGAGGAG GAGGTGACTGCTGTGGATGGTTATGAGACAGACCACCAGGACTATTGCGAGGTGTGCCAGCAAGGCGGTG AGATCATCCTGTGTGATACCTGTCCCCGTGCTTACCACATGGTCTGCCTGGATCCCGACATGGAGAAGGC TCCCGAGGGCAAGTGGAGCTGCCCACACTGCGAGAAGGAAGGCATCCAGTGGGAAGCTAAAGAGGACAAT TCGGAGGGTGAGGAGATCCTGGAAGAGGTTGGGGGAGACCTCGAAGAGGAGGATGACCACCATATGGAAT TCTGTCGGGTCTGCAAGGATGGTGGGGAACTGCTCTGCTGTGATACCTGTCCTTCTTCCTACCACATCCA CTGCCTGAATCCCCCACTTCCAGAGATCCCCAACGGTGAATGGCTCTGTCCCCGTTGTACGTGTCCAGCT CTGAAGGGCAAAGTGCAGAAGATCCTAATCTGGAAGTGGGGTCAGCCACCATCTCCCACACCAGTGCCTC GGCCTCCAGATGCTGATCCCAACACGCCCTCCCCAAAGCCCTTGGAGGGGCGGCCAGAGCGGCAGTTCTT TGTGAAATGGCAAGGCATGTCTTACTGGCACTGCTCCTGGGTTTCTGAACTGCAGCTGGAGCTGCACTGT CAGGTGATGTTCCGAAACTATCAGCGGAAGAATGATATGGATGAGCCACCTTCTGGGGACTTTGGTGGTG ATGAAGAGAAAAGCCGAAAGCGAAAGAACAAGGACCCTAAATTTGCAGAGATGGAGGAACGCTTCTATCG CTATGGGATAAAACCCGAGTGGATGATGATCCACCGAATCCTCAACCACAGTGTGGACAAGAAGGGCCAC GTCCACTACTTGATCAAGTGGCGGGACTTACCTTACGATCAGGCTTCTTGGGAGAGTGAGGATGTGGAGA TCCAGGATTACGACCTGTTCAAGCAGAGCTATTGGAATCACAGGGAGTTAATGAGGGGTGAGGAAGGCCG ACCAGGCAAGAAGCTCAAGAAGGTGAAGCTTCGGAAGTTGGAGAGGCCTCCAGAAACGCCAACAGTTGAT CCAACAGTGAAGTATGAGCGACAGCCAGAGTACCTGGATGCTACAGGTGGAACCCTGCACCCCTATCAAA TGGAGGGCCTGAATTGGTTGCGCTTCTCCTGGGCTCAGGGCACTGACACCATCTTGGCTGATGAGATGGG CCTTGGGAAAACTGTACAGACAGCAGTCTTCCTGTATTCCCTTTACAAGGAGGGTCATTCCAAAGGCCCC TTCCTAGTGAGCGCCCCTCTTTCTACCATCATCAACTGGGAGCGGGAGTTTGAAATGTGGGCTCCAGACA TGTATGTCGTAACCTATGTGGGTGACAAGGACAGCCGTGCCATCATCCGAGAGAATGAGTTCTCCTTTGA AGACAATGCCATTCGTGGTGGCAAGAAGGCCTCCCGCATGAAGAAAGAGGCATCTGTGAAATTCCATGTG CTGCTGACATCCTATGAATTGATCACCATTGACATGGCTATTTTGGGCTCTATTGATTGGGCCTGCCTCA TCGTGGATGAAGCCCATCGGCTGAAGAACAATCAGTCTAAGTTCTTCCGGGTATTGAATGGTTACTCACT CCAGCACAAGCTGTTGCTGACTGGGACACCATTACAAAACAATCTGGAAGAGTTGTTTCATCTGCTCAAC TTTCTCACCCCCGAGAGGTTCCACAATTTGGAAGGTTTTTTGGAGGAGTTTGCTGACATTGCCAAGGAGG ACCAGATAAAAAAACTGCATGACATGCTGGGGCCGCACATGTTGCGGCGGCTCAAAGCCGATGTGTTCAA GAACATGCCCTCCAAGACAGAACTAATTGTGCGTGTGGAGCTGAGCCCTATGCAGAAGAAATACTACAAG TACATCCTCACTCGAAATTTTGAAGCACTCAATGCCCGAGGTGGTGGCAACCAGGTGTCTCTGCTGAATG TGGTGATGGATCTTAAGAAGTGCTGCAACCATCCATACCTCTTCCCTGTGGCTGCAATGGAAGCTCCTAA GATGCCTAATGGCATGTATGATGGCAGTGCCCTAATCAGAGCATCTGGGAAATTATTGCTGCTGCAGAAA ATGCTCAAGAACCTTAAGGAGGGTGGGCATCGTGTACTCATCTTTTCCCAGATGACCAAGATGCTAGACC TGCTAGAGGATTTCTTGGAACATGAAGGTTATAAATACGAACGCATCGATGGTGGAATCACTGGGAACAT GCGGCAAGAGGCCATTGACCGCTTCAATGCACCGGGTGCTCAGCAGTTCTGCTTCTTGCTTTCCACTCGA GCTGGGGGCCTTGGAATCAATCTGGCCACTGCTGACACAGTTATTATCTATGACTCTGACTGGAACCCCC ATAATGACATTCAGGCCTTTAGCAGAGCTCACCGGATTGGGCAAAATAAAAAGGTAATGATCTACCGGTT TGTGACCCGTGCGTCAGTGGAGGAGCGCATCACGCAGGTGGCAAAGAAGAAAATGATGCTGACGCATCTA GTGGTGCGGCCTGGGCTGGGCTCCAAGACTGGATCTATGTCCAAACAGGAGCTTGATGATATCCTCAAAT TTGGCACTGAGGAACTATTCAAGGATGAAGCCACTGATGGAGGAGGAGACAACAAAGAGGGAGAAGATAG CAGTGTTATCCACTACGATGATAAGGCCATTGAACGGCTGCTAGACCGTAACCAGGATGAGACTGAAGAC ACAGAATTGCAGGGCATGAATGAATATTTGAGCTCATTCAAAGTGGCCCAGTATGTGGTACGGGAAGAAG AAATGGGGGAGGAAGAGGAGGTAGAACGGGAAATCATTAAACAGGAAGAAAGTGTGGATCCTGACTACTG GGAGAAATTGCTGCGGCACCATTATGAGCAGCAGCAAGAAGATCTAGCCCGAAATCTGGGCAAAGGAAAA AGAATCCGTAAACAGGTCAACTACAATGATGGCTCCCAGGAGGACCGAGATTGGCAGGACGACCAGTCCG ACAACCAGTCCGATTACTCAGTGGCTTCAGAGGAAGGTGATGAAGACTTTGATGAACGTTCAGAAGCTCC CCGTAGGCCCAGTCGTAAGGGCCTGCGGAATGATAAAGATAAGCCATTGCCTCCTCTGTTGGCCCGTGTT GGTGGGAATATTGAAGTACTTGGTTTTAATGCTCGTCAGCGAAAAGCCTTTCTTAATGCAATTATGCGAT ATGGTATGCCACCTCAGGATGCTTTTACTACCCAGTGGCTTGTAAGAGACCTGCGAGGCAAATCAGAGAA AGAGTTCAAGGCATATGTCTCTCTTTTCATGCGGCATTTATGTGAGCCGGGGGCAGATGGGGCTGAGACC TTTGCTGATGGTGTCCCCCGAGAAGGCCTGTCTCGCCAGCATGTCCTTACTAGAATTGGTGTTATGTCTT TGATTCGCAAGAAGGTTCAGGAGTTTGAACATGTTAATGGGCGCTGGAGCATGCCTGAACTGGCTGAGGT GGAGGAAAACAAGAAGATGTCCCAGCCAGGGTCACCCTCCCCAAAAACTCCTACACCCTCCACTCCAGGG GACACGCAGCCCAACACTCCTGCACCTGTCCCACCTGCTGAAGATGGGATAAAAATAGAGGAAAATAGCC TCAAAGAAGAAGAGAGCATAGAAGGAGAAAAGGAGGTTAAATCTACAGCCCCTGAGACTGCCATTGAGTG TACACAGGCCCCTGCCCCTGCCTCAGAGGATGAAAAGGTCGTTGTTGAACCCCCTGAGGGAGAGGAGAAA GTGGAAAAGGCAGAGGTGAAGGAGAGAACAGAGGAACCTATGGAGACAGAGCCCAAAGGTGCTGCTGATG TAGAGAAGGTGGAGGAAAAGTCAGCAATAGATCTGACCCCTATTGTGGTAGAAGACAAAGAAGAGAAGAA AGAAGAAGAAGAGAAAAAAGAGGTGATGCTTCAGAATGGAGAGACCCCCAAGGACCTGAATGATGAGAAA CAGAAGAAAAATATTAAACAACGTTTCATGTTTAACATTGCAGATGGTGGTTTTACTGAGTTGCACTCCC TTTGGCAGAATGAAGAGCGGGCAGCCACAGTTACCAAGAAGACTTATGAGATCTGGCATCGACGGCATGA CTACTGGCTGCTAGCCGGCATTATAAACCATGGCTATGCCCGGTGGCAAGACATCCAGAATGACCCACGC TATGCCATCCTCAATGAGCCTTTCAAGGGTGAAATGAACCGTGGCAATTTCTTAGAGATCAAGAATAAAT TTCTAGCTCGAAGGTTTAAGCTCTTAGAACAAGCTCTGGTGATTGAGGAACAGCTGCGCCGGGCTGCTTA CTTGAACATGTCAGAAGACCCTTCTCACCCTTCCATGGCCCTCAACACCCGCTTTGCTGAGGTGGAGTGT TTGGCGGAAAGTCATCAGCACCTGTCCAAGGAGTCAATGGCAGGAAACAAGCCAGCCAATGCAGTCCTGC ACAAAGTTCTGAAACAGCTGGAAGAACTGCTGAGTGACATGAAAGCTGATGTGACTCGACTCCCAGCTAC CATTGCCCGAATTCCCCCAGTTGCTGTGAGGTTACAGATGTCAGAGCGTAACATTCTCAGCCGCCTGGCA AACCGGGCACCCGAACCTACCCCACAGCAGGTAGCCCAGCAGCAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001297553
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297553.1, NP_001284482.1
RefSeq Size	6404 bp
RefSeq ORF	5718 bp
Locus ID	1108
UniProt ID	Q14839
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.