NuMA (NUMA1) (NM_001286561) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NMP-22; NUMA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286561, the custom clone sequence may differ by one or more nucleotides ATGACACTCCACGCCACCCGGGGGGCTGCACTCCTCTCTTGGGTGAACAGTCTACACGTGGCTGACCCTG TGGAGGCTGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATCATTGACAGAATCCATGGCACTGA AGAGGGACAGCAAATCTTGAAGCAGCCGGTGTCAGAGAGACTGGACTTTGTGTGCAGTTTTCTGCAGAAA AATCGAAAACATCCCTCTTCCCCAGAATGCCTGGTATCTGCACAGAAGGTGCTAGAGGGATCAGAGCTGG AACTGGCGAAGATGACCATGCTGCTCTTATACCACTCTACCATGAGCTCCAAAAGTCCCAGGGACTGGGA ACAGTTTGAATATAAAATTCAGGCTGAGTTGGCTGTCATTCTTAAATTTGTGCTGGACCATGAGGACGGG CTAAACCTTAATGAGGACCTAGAGAACTTCCTACAGAAAGCTCCTGTGCCTTCTACCTGTTCTAGCACAT TCCCTGAAGAGCTCTCCCCACCTAGCCACCAGGCCAAGAGGGAGATTCGCTTCCTAGAGCTACAGAAGGT TGCCTCCTCTTCCAGTGGGAACAACTTTCTCTCAGGTTCTCCAGCTTCTCCCATGGGTGATATCCTGCAG ACCCCACAGTTCCAGATGAGACGGCTGAAGAAGCAGCTTGCTGATGAGAGAAGTAATAGGGATGAGCTGG AGCTGGAGCTAGCTGAGAACCGCAAGCTCCTCACCGAGAAGGATGCACAGATAGCCATGATGCAGCAGCG CATTGACCGCCTAGCCCTGCTGAATGAGAAGCAGGCGGCCAGCCCACTGGAGCCCAAGGAGCTTGAGGAG CTGCGTGACAAGAATGAGAGCCTTACCATGCGGCTGCATGAAACCCTGAAGCAGTGCCAGGACCTGAAGA CAGAGAAGAGCCAGATGGATCGCAAAATCAACCAGCTTTCGGAGGAGAATGGAGACCTTTCCTTTAAGCT GCGGGAGTTTGCCAGTCATCTGCAGCAGCTACAGGATGCCCTCAATGAGCTGACGGAGGAGCACAGCAAG GCCACTCAGGAGTGGCTAGAGAAGCAGGCCCAGCTGGAGAAGGAGCTCAGCGCAGCCCTGCAGGACAAGA AATGCCTTGAAGAGAAGAACGAAATCCTTCAGGGAAAACTTTCACAGCTGGAAGAACACTTGTCCCAGCT GCAGGATAACCCACCCCAGGAGAAGGGCGAGGTGCTGGGTGATGTCTTGCAGCTGGAAACCTTGAAGCAA GAGGCAGCCACTCTTGCTGCAAACAACACACAGCTCCAAGCCAGGGTAGAGATGCTGGAGACTGAGCGAG GCCAGCAGGAAGCCAAGCTGCTTGCTGAGCGGGGCCACTTCGAAGAAGAAAAGCAGCAGCTGTCTAGCCT GATCACTGACCTGCAGAGCTCCATCTCCAACCTCAGCCAGGCCAAGGAAGAGCTGGAGCAGGCCTCCCAG GCTCATGGGGCCCGGTTGACTGCCCAGGTGGCCTCTCTGACCTCTGAGCTCACCACACTCAATGCCACCA TCCAGCAACAGGATCAAGAACTGGCTGGCCTGAAGCAGCAGGCCAAAGAGAAGCAGGCCCAGCTAGCACA GACCCTCCAACAGCAAGAACAGGCCTCCCAGGGCCTCCGCCACCAGGTGGAGCAGCTAAGCAGTAGCCTG AAGCAGAAGGAGCAGCAGTTGAAGGAGGTAGCGGAGAAGCAGGAGGCAACTAGGCAGGACCATGCCCAGC AACTGGCCACTGCTGCAGAGGAGCGAGAGGCCTCCTTAAGGGAGCGGGATGCGGCTCTCAAGCAGCTGGA GGCACTGGAGAAGGAGAAGGCTGCCAAGCTGGAGATTCTGCAGCAGCAACTTCAGGTGGCTAATGAAGCC CGGGACAGTGCCCAGACCTCAGTGACACAGGCCCAGCGGGAGAAGGCAGAGCTGAGCCGGAAGGTGGAGG AACTCCAGGCCTGTGTTGAGACAGCCCGCCAGGAACAGCATGAGGCCCAGGCCCAGGTTGCAGAGCTAGA GTTGCAGCTGCGGTCTGAGCAGCAAAAAGCAACTGAGAAAGAAAGGGTGGCCCAGGAGAAGGACCAGCTC CAGGAGCAGCTCCAGGCCCTCAAAGAGTCCTTGAAGGTCACCAAGGGCAGCCTTGAAGAGGAGAAGCGCA GGGCTGCAGATGCCCTGGAAGAGCAGCAGCGTTGTATCTCTGAGCTGAAGGCAGAGACCCGAAGCCTGGT GGAGCAGCATAAGCGGGAACGAAAGGAGCTGGAAGAAGAGAGGGCTGGGCGCAAGGGGCTGGAGGCTCGA TTACAGCAGCTTGGGGAGGCCCATCAGGCTGAGACTGAAGTCCTGCGGCGGGAGCTGGCAGAGGCCATGG CTGCCCAGCACACAGCTGAGAGTGAGTGTGAGCAGCTCGTCAAAGAAGTAGCTGCCTGGCGTGAGCGGTA TGAGGATAGCCAGCAAGAGGAGGCACAGTATGGCGCCATGTTCCAGGAACAGCTGATGACTTTGAAGGAG GAATGTGAGAAGGCCCGCCAGGAGCTGCAGGAGGCAAAGGAGAAGGTGGCAGGCATAGAATCCCACAGCG AGCTCCAGATAAGCCGGCAGCAGAACGAACTAGCTGAGCTCCATGCCAACCTGGCCAGAGCACTCCAGCA GGTCCAAGAGAAGGAAGTCAGGGCCCAGAAGCTTGCAGATGACCTCTCCACTCTGCAGGAAAAGATGGCT GCCACCAGCAAAGAGGTGGCCCGCTTGGAGACCTTGGTGCGCAAGGCAGGTGAGCAGCAGGAAACAGCCT CCCGGGAGTTAGTCAAGGAGCCTGCGAGGGCAGGAGACAGACAGCCCGAGTGGCTGGAAGAGCAACAGGG ACGCCAGTTCTGCAGCACACAGGCAGCGCTGCAGGCTATGGAGCGGGAGGCAGAGCAGATGGGCAATGAG CTGGAACGGCTGCGGGCCGCGCTGATGGAGAGCCAGGGGCAGCAGCAGGAGGAGCGTGGGCAGCAGGAAA GGGAGGTGGCGCGGCTGACCCAGGAGCGGGGCCGTGCCCAGGCTGACCTTGCCCTGGAGAAGGCGGCCAG AGCAGAGCTTGAGATGCGGCTGCAGAACGCCCTCAACGAGCAGCGTGTGGAGTTCGCTACCCTGCAAGAG GCACTGGCTCATGCCCTGACGGAAAAGGAAGGCAAGGACCAGGAGTTGGCCAAGCTTCGTGGTCTGGAGG CAGCCCAGATAAAAGAGCTGGAGGAACTTCGGCAAACCGTGAAGCAACTGAAGGAACAGCTGGCTAAGAA AGAAAAGGAGCACGCATCTGGCTCAGGAGCCCAATCTGAGGCTGCTGGCAGGACAGAGCCAACAGGCCCC AAGCTGGAGGCACTGCGGGCAGAGGTGAGCAAGCTGGAACAGCAATGCCAGAAGCAGCAGGAGCAGGCTG ACAGCCTGGAACGCAGCCTCGAGGCTGAGCGGGCCTCCCGGGCTGAGCGGGACAGTGCTCTGGAGACTCT GCAGGGCCAGTTAGAGGAGAAGGCCCAGGAGCTAGGGCACAGTCAGAGTGCCTTAGCCTCGGCCCAACGG GAGTTGGCTGCCTTCCGCACCAAGGTACAAGACCACAGCAAGGCTGAAGATGAGTGGAAGGCCCAGGTGG CCCGGGGCCGGCAAGAGGCTGAGAGGAAAAATAGCCTCATCAGCAGCTTGGAGGAGGAGGTGTCCATCCT GAATCGCCAGGTCCTGGAGAAGGAGGGGGAGAGCAAGGAGTTGAAGCGGCTGGTGATGGCCGAGTCAGAG AAGAGCCAGAAGCTGGAGGAGAGGCTGCGCCTGCTGCAGGCAGAGACAGCCAGCAACAGTGCCAGAGCTG CAGAACGCAGCTCTGCTCTGCGGGAGGAGGTGCAGAGCCTCCGGGAGGAGGCTGAGAAACAGCGGGTGGC TTCAGAGAACCTGCGGCAGGAGCTGACCTCACAGGCTGAGCGTGCGGAGGAGCTGGGCCAAGAATTGAAG GCGTGGCAGGAGAAGTTCTTCCAGAAAGAGCAGGCCCTCTCCACCCTGCAGCTCGAGCACACCAGCACAC AGGCCCTGGTGAGTGAGCTGCTGCCAGCTAAGCACCTCTGCCAGCAGCTGCAGGCCGAGCAGGCCGCTGC CGAGAAACGCCACCGTGAGGAGCTGGAGCAGAGCAAGCAGGCCGCTGGGGGACTGCGGGCAGAGCTGCTG CGGGCCCAGCGGGAGCTTGGGGAGCTGATTCCTCTGCGGCAGAAGGTGGCAGAGCAGGAGCGAACAGCTC AGCAGCTGCGGGCAGAGAAGGCCAGCTATGCAGAGCAGCTGAGCATGCTGAAGAAGGCGCATGGCCTGCT GGCAGAGGAGAACCGGGGGCTGGGTGAGCGGGCCAACCTTGGCCGGCAGTTTCTGGAAGTGGAGTTGGAC CAGGCCCGGGAGAAGTATGTCCAAGAGTTGGCAGCCGTACGTGCTGATGCTGAGACCCGTCTGGCTGAGG TGCAGCGAGAAGCACAGAGCACTGCCCGGGAGCTGGAGGTGATGACTGCCAAGTATGAGGGTGCCAAGGT CAAGGTCCTGGAGGAGAGGCAGCGGTTCCAGGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAAGAACTG AGTAAGAAACTGGCTGACTCTGACCAAGCCAGCAAGGTGCAGCAGCAGAAGCTGAAGGCTGTCCAGGCTC AGGGAGGCGAGAGCCAGCAGGAGGCCCAGCGCCTCCAGGCCCAGCTGAATGAACTGCAAGCCCAGTTGAG CCAGAAGGAGCAGGCAGCTGAGCACTATAAGCTGCAGATGGAGAAAGCCAAAACACATTATGATGCCAAG AAGCAGCAGAACCAAGAGCTGCAGGAGCAGCTGCGGAGCCTGGAGCAGCTGCAGAAGGAAAACAAAGAGC TGCGAGCTGAAGCTGAACGGCTGGGCCATGAGCTACAGCAGGCTGGGCTGAAGACCAAGGAGGCTGAACA GACCTGCCGCCACCTTACTGCCCAGGTGCGCAGCCTGGAGGCACAGGTTGCCCATGCAGACCAGCAGCTT CGAGACCTGGGCAAATTCCAGGTGGCAACTGATGCTTTAAAGAGCCGTGAGCCCCAGGCTAAGCCCCAGC TGGACTTGAGTATTGACAGCCTGGATCTGAGCTGCGAGGAGGGGACCCCACTCAGTATCACCAGCAAGCT GCCTCGTACCCAGCCAGACGGCACCAGCGTCCCTGGAGAACCAGCCTCACCTATCTCCCAGCGCCTGCCC CCCAAGGTAGAATCCCTGGAGAGTCTCTACTTCACTCCCATCCCTGCTCGGAGTCAGGCCCCCCTGGAGA GCAGCCTGGACTCCCTGGGAGACGTCTTCCTGGACTCGGGTCGTAAGACCCGCTCCGCTCGTCGGCGCAC CACGCAGATCATCAACATCACCATGACCAAGAAGCTAGATGTGGAAGAGCCAGACAGCGCCAACTCATCG TTCTACAGCACGCGGTCTGCTCCTGCTTCCCAGGCTAGCCTGCGAGCCACCTCCTCTACTCAGTCTCTAG CTCGCCTGGGTTCTCCCGATTATGGCAACTCAGCCCTGCTCAGCTTGCCTGGCTACCGCCCCACCACTCG CAGTTCTGCTCGTCGTTCCCAGGCCGGGGTGTCCAGTGGGGCCCCTCCAGGAAGGAACAGCTTCTACATG GGCACTTGCCAGGATGAGCCTGAGCAGCTGGATGACTGGAACCGCATTGCAGAGCTGCAGCAGCGCAATC GAGTGTGCCCCCCACATCTGAAGACCTGCTATCCCCTGGAGTCCAGGCCTTCCCTGAGCCTGGGCACCAT CACAGATGAGGAGATGAAAACTGGAGACCCCCAAGAGACCCTGCGCCGAGCCAGCATGCAGCCAATCCAG ATAGCCGAGGGCACTGGCATCACCACCCGGCAGCAGCGCAAACGGGTCTCCCTAGAGCCCCACCAGGGCC CTGGAACTCCTGAGTCTAAGAAGGCCACCAGCTGTTTCCCACGCCCCATGACTCCCCGAGACCGACATGA AGGGCGCAAACAGAGCACTACTGAGGCCCAGAAGAAAGCAGCTCCAGCTTCTACTAAACAGGCTGACCGG CGCCAGTCGATGGCCTTCAGCATCCTCAACACACCCAAGAAGCTAGGGAACAGCCTTCTGCGGCGGGGAG CCTCAAAGAAGGCCCTGTCCAAGGCTTCCCCCAACACTCGCAGTGGAACCCGCCGTTCTCCGCGCATTGC CACCACCACAGCCAGCGCCGCCACTGCTGCCGCCATTGGTGCCACCCCTCGAGCCAAGGGCAAGGCAAAG CACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286561
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286561.1, NP_001273490.1
RefSeq Size	7431 bp
RefSeq ORF	6306 bp
Locus ID	4926
UniProt ID	Q14980
Gene Summary	This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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