Phospholipase C epsilon 1 (PLCE1) (NM_001288989) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NPHS3; PLCE; PPLC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288989, the custom clone sequence may differ by one or more nucleotides ATGACTTCTGAAGAAATGACAGCTTCTGTTCTCATACCTGTGACTCAGAGAAAAGTGGTTTCTGCCCAGT CGGCTGCAGATGAAAGTAGTGAAAAGGTCTCAGACATCAATATTTCAAAAGCACATACTGTCAGACGAAG TGGGGAGACTTCTCATACCATCTCACAACTGAACAAACTTAAAGAAGAACCTTCTGGAAGCAACTTGCCA AAGATTCTCTCAATAGCGAGGGAGAAAATAGTGAGTGATGAGAACAGTAATGAAAAATGTTGGGAGAAAA TCATGCCAGATTCTGCGAAAAACCTTAACATTAACTGCAACAACATATTGAGAAACCATCAGCATGGCCT TCCTCAGAGACAATTTTATGAAATGTACAACTCTGTTGCTGAGGAAGACTTGTGTTTAGAAACTGGAATT CCTTCTCCACTGGAAAGAAAGGTGTTCCCTGGAATTCAACTGGAACTAGACAGACCTTCCATGGGCATTA GTCCTTTAGGAAATCAGTCAGTGATCATAGAGACAGGCAGAGCACACCCTGACAGCAGAAGGGCAGTATT TCATTTTCATTATGAAGTTGACAGAAGAATGTCAGACACTTTCTGTACCCTATCAGAAAACTTAATTTTA GACGATTGTGGAAATTGTGTACCACTACCTGGGGGTGAGGAGAAGCAAAAGAAAAACTATGTGGCATATA CCTGTAAACTGATGGAATTGGCCAAAAATTGTGATAATAAGAATGAGCAGCTGCAGTGTGATCATTGTGA CACCTTGAATGATAAATACTTTTGCTTTGAAGGCTCTTGTGAGAAGGTTGACATGGTATATTCAGGTGAT AGCTTTTGTAGGAAAGACTTTACTGACAGTCAAGCTGCCAAGACCTTTTTGAGCCATTTTGAGGACTTCC CTGATAATTGTGATGATGTAGAAGAAGACGCTTTTAAAAGCAAAAAGGAGCGATCCACTTTGTTAGTCAG GAGATTCTGTAAAAATGACAGAGAAGTTAAGAAATCTGTGTATACTGGAACAAGAGCAATTGTGAGAACT CTGCCTTCTGGCCACATTGGGCTGACTGCATGGAGTTACATAGATCAGAAGAGAAATGGTCCCTTACTGC CTTGTGGGAGAGTAATGGAACCCCCGTCAACAGTGGAGATAAGGCAAGATGGGAGCCAACGTCTGTCAGA AGCCCAGTGGTATCCTATCTACAATGCAGTGAGAAGAGAAGAAACAGAAAATACAGTTGGATCTCTACTC CATTTCCTCACCAAGCTCCCAGCCTCCGAGACAGCCCATGGAAGGATAAGCGTTGGTCCATGCTTAAAGC AATGTGTCCGAGACACTGTATGTGAGTATCGCGCCACCCTCCAAAGGACTTCAATATCGCAGTACATCAC CGGTTCTCTCCTAGAAGCAACCACGTCTTTGGGAGCAAGAAGTGGCCTTCTCAGTACTTTTGGAGGATCC ACTGGACGAATGATGCTGAAAGAACGCCAGCCAGGCCCCTCTGTGGCCAATTCCAATGCCCTCCCTTCAA GTTCAGCTGGGATCAGCAAGGAGCTGATCGATCTGCAGCCTCTCATCCAGTTCCCAGAGGAAGTCGCCAG CATCCTGATGGAGCAAGAGCAGACTATTTACCGCAGGGTCTTGCCAGTCGACTACCTTTGCTTCTTAACA CGGGACTTGGGCACTCCTGAATGCCAGAGCTCCTTGCCCTGCCTCAAAGCATCCATCTCAGCGTCGATTC TTACCACTCAGAATGGAGAGCACAATGCCCTTGAAGATCTGGTGATGAGGTTTAATGAGGTGAGCTCCTG GGTGACATGGCTGATCCTCACGGCAGGCTCCATGGAGGAGAAGCGAGAAGTCTTTTCATATTTGGTGCAT GTGGCCAAATGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCTTGGCTGGCCTCAGGTCAA GAAAAGTTTTAAAAATGTGGCAGTTCATGGACCAGTCTGATATTGAGACCATGAGGAGCCTGAAGGATGC TATGGCCCAGCATGAGTCCTCTTGTGAGTACAGAAAGGTGGTGACACGTGCCCTGCACATCCCTGGCTGT AAGGTGGTTCCATTCTGTGGGGTGTTTCTGAAGGAGCTCTGTGAAGTGCTTGACGGCGCCTCCGGTCTCA TGAAGCTTTGCCCGCGGTACAATTCCCAAGAAGAAACTTTAGAGTTTGTAGCAGATTACAGTGGACAAGA TAATTTCTTACAACGAGTGGGACAAAATGGCTTAAAGAATTCGGAGAAGGAGTCCACTGTCAACAGCATC TTTCAGGTCATCCGGAGCTGCAATCGAAGTCTGGAGACAGACGAGGAGGACAGCCCCAGTGAAGGAAACA GCTCCAGGAAAAGCTCCTTGAAGGATAAAAGCCGATGGCAGTTTATAATTGGAGATTTGTTGGATTCAGA CAATGACATCTTTGAGCAATCCAAAGAATACGACTCTCATGGTTCAGAGGACTCACAGAAGGCCTTCGAC CATGGGACGGAGCTCATCCCTTGGTACGTGCTGTCCATCCAAGCCGATGTGCACCAGTTCCTGCTGCAGG GGGCCACGGTCATCCACTACGACCAGGACACACACCTCTCTGCCCGCTGCTTCCTCCAGCTTCAGCCCGA CAATAGCACCTTGACCTGGGTAAAGCCCACAACTGCCTCCCCAGCCAGCAGTAAAGCAAAACTTGGTGTA CTTAATAACACAGCTGAGCCTGGAAAATTCCCACTACTGGGTAATGCTGGATTAAGTAGCCTGACGGAAG GGGTCTTGGATCTTTTTGCAGTGAAGGCTGTATACATGGGCCACCCTGGCATTGATATACACACTGTGTG TGTTCAGAACAAACTGGGTAGCATGTTCCTGTCAGAGACTGGTGTGACATTGCTCTATGGGCTTCAGACC ACAGACAACAGATTATTGCACTTCGTGGCACCAAAGCACACAGCTAAAATGCTCTTCAGCGGATTATTGG AACTCACTAGAGCTGTGAGAAAGATGAGGAAATTCCCTGACCAAAGACAGCAGTGGCTGCGGAAACAGTA CGTCAGCCTTTATCAGGAGGATGGACGGTATGAAGGCCCAACTTTGGCTCACGCTGTGGAGTTGTTTGGT GGCAGACGGTGGAGTGCTCGAAACCCCAGCCCCGGAACATCAGCAAAGAATGCTGAGAAGCCCAATATGC AGAGAAACAATACCCTGGGCATAAGCACTACCAAGAAAAAGAAGAAAATCCTCATGAGGGGTGAGAGTGG AGAGGTAACTGACGATGAGATGGCAACCCGAAAGGCCAAGATGCACAAAGAGTGTCGAAGCCGGAGTGGT TCTGATCCTCAAGACATTAATGAACAAGAAGAATCAGAGGTGAATGCCATCGCTAACCCTCCAAACCCCC TCCCTTCCAGAAGAGCCCACTCTTTGACCACAGCTGGGTCCCCCAACTTGGCTGCCGGGACGTCATCTCC CATCAGTGCTTGGAGCAGTAGTAGCTGGCACGGGCGGATCAAAGGCGGCATGAAGGGATTTCAGAGCTTC ATGGTTTCAGATAGCAACATGAGTTTTGTTGAATTTGTTGAGCTGTTCAAATCATTCAGTGTCAGGAGCC GCAAGGACCTGAAGGATCTGTTTGATGTCTATGCAGTGCCCTGCAACCGATCTGGCTCCGAGTCAGCCCC ACTCTACACCAACCTGACAATTGATGAAAACACCAGCGATCTTCAGCCTGACCTAGATCTGTTGACCAGA AATGTCTCGGATTTGGGGTTGTTCATTAAGAGTAAACAGCAGCTATCGGACAACCAGAGGCAGATATCTG ATGCCATTGCTGCTGCAAGCATTGTGACAAATGGCACTGGGATTGAGAGCACATCTCTGGGCATTTTTGG GGTGGGCATACTTCAGCTCAACGATTTCCTCGTGAATTGCCAAGGAGAACACTGCACTTATGATGAAATC CTCAGCATCATCCAGAAGTTCGAGCCTAGCATCAGTATGTGTCATCAGGGACTAATGTCATTTGAAGGGT TTGCCAGGTTTCTGATGGATAAAGAAAATTTTGCCTCAAAAAATGATGAGTCACAGGAGAACATTAAAGA ACTGCAGCTACCCCTCTCATACTATTACATCGAATCTTCGCACAATACCTACCTCACGGGCCATCAGCTC AAAGGAGAATCCTCGGTAGAACTCTACAGCCAGGTCCTTTTGCAAGGCTGTCGAAGTGTAGAATTGGACT GCTGGGACGGAGACGATGGGATGCCCATCATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAA GGAAGTGGTTGAAGCCATTGATCGCAGTGCCTTCATCAACTCTGACCTGCCAATCATCATATCGATTGAG AACCACTGTTCATTGCCTCAGCAACGAAAAATGGCAGAAATTTTCAAGACTGTGTTTGGAGAAAAGCTGG TGACTAAATTCTTATTTGAGACTGATTTCTCAGATGATCCAATGCTTCCTTCACCTGACCAACTCAGAAA GAAAGTTCTTCTTAAAAACAAGAAGCTAAAAGCCCATCAGACGCCAGTGGATATCTTAAAGCAAAAGGCT CATCAGTTAGCATCTATGCAAGTGCAGGCTTATAATGGTGGGAATGCCAACCCCCGACCTGCCAATAATG AGGAAGAGGAAGATGAGGAGGACGAATATGATTATGACTATGAATCCCTTTCTGATGACAACATTCTGGA AGACAGACCTGAAAATAAATCATGTAATGACAAGCTTCAGTTTGAATATAATGAAGAAATCCCAAAGAGG ATAAAGAAAGCAGATAACTCTGCTTGCAACAAAGGAAAGGTTTATGATATGGAACTGGGAGAAGAATTTT ATCTTGATCAGAATAAAAAGGAAAGCAGACAGATTGCACCAGAGCTTTCTGACCTTGTAATCTATTGTCA AGCAGTAAAATTTCCAGGACTGTCAACTCTAAATGCATCTGGCTCTAGCAGAGGAAAAGAAAGGAAAAGC AGGAAGTCCATTTTTGGCAACAATCCGGGCAGAATGAGCCCAGGGGAGACAGCATCATTTAACAAAACAT CTGGAAAAAGTTCCTGTGAAGGCATTCGACAGACCTGGGAGGAATCTTCTTCCCCTCTCAACCCAACCAC GTCCCTCAGTGCTATCATTAGAACTCCCAAATGTTATCATATCTCGTCGCTGAATGAAAATGCCGCCAAA CGTCTGTGTCGCAGGTATTCTCAGAAACTGACCCAGCACACCGCCTGTCAGCTGCTGAGAACTTACCCTG CTGCCACCCGCATCGACTCTTCCAACCCGAACCCCCTCATGTTCTGGCTCCATGGGATACAGCTTGTGGC ACTCAACTACCAGACTGATGATCTCCCTTTACATTTAAATGCTGCAATGTTTGAGGCAAATGGTGGTTGT GGTTATGTATTGAAACCTCCAGTTCTGTGGGACAAGAACTGCCCCATGTATCAGAAGTTTTCTCCACTAG AAAGAGATCTGGACAGCATGGATCCTGCAGTCTATTCTTTAACTATTGTCTCTGGTCAGAATGTGTGCCC CAGTAATAGCATGGGAAGCCCGTGCATTGAAGTCGACGTCCTGGGCATGCCTCTGGACAGCTGCCATTTC CGCACAAAGCCCATCCATCGAAACACCCTGAACCCCATGTGGAACGAGCAGTTTCTGTTCCACGTTCACT TCGAAGATCTTGTATTTCTTCGTTTTGCAGTTGTGGAAAACAATAGTTCAGCGGTAACTGCTCAGAGAAT CATTCCACTGAAAGCTTTAAAACGAGGATATCGACATCTTCAGCTGCGAAACCTTCACAATGAAGTCTTG GAGATTTCTAGTTTATTCATTAACAGCAGAAGGATGGAAGAAAATTCCTCTGGCAATACCATGTCAGCCT CTTCGATGTTTAATACAGAAGAAAGAAAATGTTTGCAGACTCACAGAGTCACGGTGCATGGGGTCCCAGG GCCAGAGCCCTTTACCGTTTTCACTATTAATGGAGGCACCAAGGCAAAGCAGCTTCTGCAGCAAATTCTG ACAAATGAACAAGACATCAAACCTGTTACCACAGACTATTTTTTGATGGAAGAAAAATATTTTATATCTA AAGAAAAGAATGAATGTAGGAAACAACCATTCCAGAGAGCCATTGGTCCAGAAGAGGAGATCATGCAAAT TTTAAGCAGCTGGTTTCCAGAAGAGGGATACATGGGCAGGATTGTCTTAAAAACCCAGCAGGAAAACCTA GAAGAGAAAAACATTGTTCAAGATGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTCC AAGTGCATGATGTTTCTCCAGAGCAACCTCGAACAGTCATCAAAGCACCCCGCGTCAGCACTGCACAGGA TGTCATTCAGCAGACCTTATGCAAAGCCAAATATTCCTACAGCATCCTGAGCAACCCCAATCCAAGCGAC TATGTGCTTTTGGAAGAGGTGGTGAAAGACACTACCAACAAGAAGACTACCACACCAAAGTCCTCTCAGC GGGTCCTTCTGGATCAGGAGTGTGTGTTTCAAGCCCAAAGCAAGTGGAAAGGTGCAGGAAAATTCATCCT TAAGCTAAAGGAGCAGGTGCAGGCATCTCGAGAAGATAAAAAGAAAGGCATTTCTTTCGCAAGTGAACTC AAGAAGCTCACCAAGTCAACTAAACAGCCCCGAGGACTTACATCACCTTCTCAGCTCTTGACCTCAGAAA GTATCCAAACCAAGGAGGAGAAACCTGTGGGTGGCTTGTCCTCCAGTGACACAATGGATTACCGACAGTG A
Restriction Sites	SgfI-MluI
ACCN	NM_001288989
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288989.1, NP_001275918.1
RefSeq Size	7957 bp
RefSeq ORF	6861 bp
Locus ID	51196
UniProt ID	Q9P212
Protein Families	Druggable Genome
Protein Pathways	Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
Gene Summary	This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.