III (AC_000007) Virus Tagged ORF Clone

CAT#: VC100039

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 2], codon optimized for human cell expression, AP_000170



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CNY 6,940.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100039 represents NCBI reference of AP_000170 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGAGCCGCAATGTACGAGGAGGGACCACCACCTAGCTACGAGTCAGTCGTAAGTGCCGCCCCTG
TCGCTGCCGCTCTTGGGTCTCCCTTCGACGCCCCTCTTGACCCTCCTTTCGTTCCACCAAGATACCTGCG
CCCAACCGGAGGAAGGAACAGTATCCGCTATTCCGAGCTTGCCCCTTTGTTCGATACTACCAGGGTTTAC
CTTGTGGACAACAAGTCTACTGACGTTGCAAGCCTCAACTATCAGAACGATCACTCTAATTTTCTGACTA
CAGTCATCCAGAATAATGACTACTCTCCAGGTGAGGCGTCTACACAGACAATTAATTTGGATGATCGATC
TCATTGGGGCGGTGACCTTAAGACGATTCTGCATACCAATATGCCTAACGTAAATGAATTCATGTTCACC
AACAAGTTTAAGGCCCGCGTAATGGTTAGTAGGAGTCTCACCAAAGACAAGCAAGTGGAGTTGAAGTATG
AGTGGGTTGAATTCACGCTGCCAGAGGGTAACTATTCCGAGACCATGACCATCGACCTGATGAACAATGC
AATCGTGGAACATTATCTGAAGGTGGGACGGCAGAATGGAGTGCTTGAGTCTGATATCGGGGTTAAGTTT
GATACTAGGAACTTCCGCCTGGGGTTCGACCCCGTGACTGGCCTGGTAATGCCAGGCGTGTATACAAACG
AGGCCTTCCATCCCGATATCATCCTTCTGCCCGGATGTGGCGTGGACTTCACCCACTCCAGGCTGAGCAA
CCTGCTGGGCATCAGGAAAAGGCAGCCCTTCCAGGAGGGATTCCGAATCACTTATGATGATCTTGAAGGC
GGAAACATCCCAGCTCTGCTCGACGTCGATGCTTACCAGGCTAGCCTGAAGGACGACACAGAACAAGGCG
GCGATGGAGCCGGCGGGGGAAACAACAGCGGCAGCGGCGCAGAAGAAAACAGTAATGCCGCAGCTGCCGC
CATGCAGCCAGTAGAAGATATGAACGATCATGCCATTAGGGGAGACACTTTCGCTACTCGGGCTGAGGAA
AAAAGGGCCGAAGCCGAAGCAGCCGCCGAGGCCGCCGCTCCAGCTGCGCAGCCCGAAGTCGAAAAGCCCC
AGAAAAAGCCTGTGATCAAGCCACTGACAGAGGATTCAAAAAAACGGAGTTACAACCTGATCTCAAATGA
CTCAACTTTCACCCAATATCGAAGCTGGTACCTGGCCTACAACTACGGGGACCCACAAACAGGCATCAGG
TCCTGGACACTTCTGTGTACCCCAGACGTGACATGCGGTTCAGAACAGGTATACTGGTCTCTCCCCGACA
TGATGCAAGACCCTGTCACATTCAGATCAACCTCACAAATATCAAATTTTCCAGTGGTAGGCGCTGAACT
CCTGCCAGTGCACTCAAAATCTTTTTACAATGATCAAGCAGTGTATTCCCAGCTCATTCGCCAATTTACT
TCACTTACCCATGTGTTCAACAGGTTCCCCGAAAACCAAATACTGGCACGCCCGCCTGCCCCCACAATCA
CCACAGTGTCCGAGAACGTGCCGGCCCTCACCGATCACGGAACCCTGCCACTGCGCAACTCCATTGGTGG
TGTTCAAAGAGTCACAATAACTGACGCGCGGAGGCGGACCTGCCCCTACGTGTATAAGGCACTCGGGATC
GTCTCTCCACGGGTGCTCTCTAGCCGGACATTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100039 representing AP_000170
Red=Cloning sites Green=Tags

MQRAAMYEEGPPPSYESVVSAAPVAAALGSPFDAPLDPPFVPPRYLRPTGGRNSIRYSELAPLFDTTRVY
LVDNKSTDVASLNYQNDHSNFLTTVIQNNDYSPGEASTQTINLDDRSHWGGDLKTILHTNMPNVNEFMFT
NKFKARVMVSRSLTKDKQVELKYEWVEFTLPEGNYSETMTIDLMNNAIVEHYLKVGRQNGVLESDIGVKF
DTRNFRLGFDPVTGLVMPGVYTNEAFHPDIILLPGCGVDFTHSRLSNLLGIRKRQPFQEGFRITYDDLEG
GNIPALLDVDAYQASLKDDTEQGGDGAGGGNNSGSGAEENSNAAAAAMQPVEDMNDHAIRGDTFATRAEE
KRAEAEAAAEAAAPAAQPEVEKPQKKPVIKPLTEDSKKRSYNLISNDSTFTQYRSWYLAYNYGDPQTGIR
SWTLLCTPDVTCGSEQVYWSLPDMMQDPVTFRSTSQISNFPVVGAELLPVHSKSFYNDQAVYSQLIRQFT
SLTHVFNRFPENQILARPPAPTITTVSENVPALTDHGTLPLRNSIGGVQRVTITDARRRTCPYVYKALGI
VSPRVLSSRTF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000007
ORF Size 1713 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000007.1, AP_000170
RefSeq ORF 1713 bp
UniProt ID P03254
MW 63.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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