L3 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100303

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus B], codon optimized for human cell expression, YP_002213812



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CNY 14,250.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100303 represents NCBI reference of YP_002213812 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAACCCCCTCCATGTTGCCGCAGTGGGCATACATGCACATCGCCGGACAAGACGCTAGCGAATACT
TGAGTCCCGGACTCGTTCAGTTCGCACGGGCAACAGACACATATTTTAACCTGGGAAACAAATTTAGGAA
TCCTACGGTTGCACCGACACATGACGTCACAACCGACAGGTCCCAACGATTGATGCTTCGGTTCGTACCA
GTTGATAGGGAAGATAACACCTATAGTTATAAGGTGCGCTACACTCTTGCCGTTGGAGATAATAGGGTGC
TGGACATGGCTTCTACATTCTTTGATATTAGGGGTGTGCTGGACAGAGGGCCAAGCTTCAAACCGTACTC
AGGCACAGCCTATAACAGTTTGGCTCCTAAAGGGGCTCCAAATACATCACAGTGGATTGCCGAGGGAGTA
AAAAATACAACTGGTGAAGAGCATGTTACGGAGGAGGAGACCAACACTACAACCTACACGTTCGGCAATG
CCCCCGTCAAAGCCGAGGCCGAGATCACTAAGGAGGGTCTCCCTGTGGGCCTTGAAGTGTCAGACGAGGA
GAGCAAGCCCATTTACGCAGACAAAACTTACCAACCAGAACCCCAACTGGGTGACGAGACATGGACTGAT
CTGGATGGCAAGACCGAGAAATACGGCGGAAGGGCTTTGAAACCCGACACCAAGATGAAGCCATGTTATG
GGTCCTTCGCGAAACCTACCAATGTCAAAGGGGGGCAGGCAAAGCAGAAGACCACGGAGCAGCCCAATCA
GAAGGTCGAATATGACATAGATATGGAATTTTTCGACGCAGCCAGCCAGAAGACTAACCTGTCACCTAAG
ATTGTTATGTATGCAGAGAACGTGAACCTGGAAACGCCGGACACCCATGTGGTCTATAAGCCCGGTACAG
AAGATACATCTTCTGAGGCCAACCTGGGTCAACAGAGTATGCCTAACAGACCAAATTACATCGGCTTCCG
CGACAATTTTATTGGGTTGATGTATTATAATAGCACCGGTAACATGGGGGTTCTTGCCGGTCAGGCATCA
CAGCTGAACGCCGTAGTAGATCTCCAGGATAGAAATACCGAGCTGTCATACCAGCTCTTGCTGGATAGTT
TGGGCGACCGCACACGCTACTTTAGTATGTGGAACCAGGCAGTCGATTCCTATGACCCAGATGTGCGGGT
GATAGAGAACCATGGCGTAGAGGACGAGCTGCCAAATTACTGTTTTCCTCTGGATGGCATTGGTGTGCCT
ACGACATCTTACAAGTCCATTGTCCCCAATGGTGATAACGCCCCCAATTGGAAGGAGCCCGAGGTTAACG
GCACATCAGAAATCGGCCAGGGCAATCTTTTTGCCATGGAGATTAATCTCCAGGCCAACCTTTGGCGCAG
CTTCCTGTATTCAAATGTTGCACTGTACCTCCCTGATAGTTATAAGTACACCCCATCCAATGTGACTCTC
CCCGAAAACAAGAATACATACGATTATATGAATGGCCGGGTCGTTCCTCCTTCCCTTGTTGACACATACG
TAAATATTGGCGCACGGTGGAGCCTTGACGCGATGGACAATGTCAACCCTTTCAACCACCATAGGAACGC
TGGTTTGCGCTATCGGTCCATGCTGCTGGGAAACGGGCGATATGTGCCGTTCCACATTCAAGTGCCCCAG
AAGTTTTTTGCCGTCAAGAATCTGCTTCTGTTGCCCGGCTCTTATACTTATGAGTGGAACTTCCGGAAGG
ACGTGAACATGGTTCTTCAGTCCTCTTTGGGCAATGATCTTAGAGTGGACGGCGCCAGCATATCTTTTAC
CAGCATCAACCTCTATGCGACCTTTTTTCCTATGGCTCATAACACAGCTAGCACATTGGAGGCCATGCTG
CGCAATGATACAAACGACCAGTCATTTAACGACTATTTGTCCGCTGCTAATATGTTGTACCCCATTCCTG
CAAACGCAACCAATATTCCAATCTCTATTCCAAGCCGCAACTGGGCCGCCTTTCGGGGTTGGAGCTTTAC
ACGGCTGAAGACTAAGGAAACTCCGTCCCTCGGGAGCGGCTTCGACCCTTATTTCGTTTATAGCGGATCA
ATCCCGTACCTGGATGGAACCTTTTATCTCAACCACACCTTCAAAAAGGTCTCCATCATGTTTGACAGCA
GTGTGAGTTGGCCCGGAAATGATAGACTCCTTTCACCCAACGAGTTTGAAATTAAACGCACAGTCGACGG
AGAGGGCTATAACGTTGCGCAGTGCAACATGACTAAGGATTGGTTCCTGGTACAGATGTTGGCAAATTAT
AACATCGGATACCAGGGCTTTTATATTCCTGAAGGGTATAAGGATAGAATGTATTCTTTCTTTCGGAATT
TCCAGCCAATGTCACGGCAGGTCGTGGACGAGGTGAACTATAAAGACTTTAAAGCCGTCGCAATTCCCTA
CCAGCATAATAACTCCGGGTTTGTGGGGTACATGGCTCCAACTATGCGGCAGGGGCAGCCGTACCCTGCA
AACTATCCCTACCCCCTTATCGGAACCACTGCCGTGAACTCAGTGACACAGAAAAAGTTCCTCTGCGACA
GAACCATGTGGCGAATCCCATTCTCTAGCAACTTTATGTCTATGGGCGCACTGACAGATCTGGGCCAGAA
CATGTTGTACGCGAACTCAGCACACGCATTGGATATGACTTTTGAAGTGGACCCCATGGATGAACCCACA
CTCCTGTATCTTCTCTTTGAGGTCTTTGATGTGGTGAGGGTGCATCAACCACACCGCGGCATAATCGAAG
CCGTGTATCTCCGGACACCTTTCTCAGCAGGCAATGCAACAACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100303 representing YP_002213812
Red=Cloning sites Green=Tags

MATPSMLPQWAYMHIAGQDASEYLSPGLVQFARATDTYFNLGNKFRNPTVAPTHDVTTDRSQRLMLRFVP
VDREDNTYSYKVRYTLAVGDNRVLDMASTFFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNTSQWIAEGV
KNTTGEEHVTEEETNTTTYTFGNAPVKAEAEITKEGLPVGLEVSDEESKPIYADKTYQPEPQLGDETWTD
LDGKTEKYGGRALKPDTKMKPCYGSFAKPTNVKGGQAKQKTTEQPNQKVEYDIDMEFFDAASQKTNLSPK
IVMYAENVNLETPDTHVVYKPGTEDTSSEANLGQQSMPNRPNYIGFRDNFIGLMYYNSTGNMGVLAGQAS
QLNAVVDLQDRNTELSYQLLLDSLGDRTRYFSMWNQAVDSYDPDVRVIENHGVEDELPNYCFPLDGIGVP
TTSYKSIVPNGDNAPNWKEPEVNGTSEIGQGNLFAMEINLQANLWRSFLYSNVALYLPDSYKYTPSNVTL
PENKNTYDYMNGRVVPPSLVDTYVNIGARWSLDAMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHIQVPQ
KFFAVKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASISFTSINLYATFFPMAHNTASTLEAML
RNDTNDQSFNDYLSAANMLYPIPANATNIPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGS
IPYLDGTFYLNHTFKKVSIMFDSSVSWPGNDRLLSPNEFEIKRTVDGEGYNVAQCNMTKDWFLVQMLANY
NIGYQGFYIPEGYKDRMYSFFRNFQPMSRQVVDEVNYKDFKAVAIPYQHNNSGFVGYMAPTMRQGQPYPA
NYPYPLIGTTAVNSVTQKKFLCDRTMWRIPFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDPMDEPT
LLYLLFEVFDVVRVHQPHRGIIEAVYLRTPFSAGNATT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_011202
ORF Size 2844 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_011202.1, YP_002213812
RefSeq ORF 2844 bp
Locus ID 6870505
MW 107.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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